Immune Response in Dendritic Cell Depleted Mice

  • Benoît Salomon
  • Pieter J. M. Leenen
  • David Klatzmann
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)

Abstract

Dendritic cells (DC) are regarded as professional antigen presenting cells capable of efficiently stimulating T cells. In addition, in vitro stimulation of naive T cells can only be achieved efficiently by dendritic cells (DC) (1–3). After antigen administration, DC are the only cells bearing immunogenic fragments and capable of stimulating specific naive T cells in vitro (4, 5). Furthermore, B cells seem dispensable in T cell-dependent immune response in vivo (6, 7). However, as yet there is no direct evidence that DC are absolutely required for the generation of an in vivo primary immune response. A possible way to approach this question is to use an animal model in which DC are depleted. There are yet no natural animal model of a genetic deficit of DC. Recently, a generated relB knock out mice have a DC deficit in lymphoid tissues. However, secondary lymphoid tissues loss their normal structure, with a strong atrophy of splenic white pulp and lymph nodes, rendering complicated the use of this model to study the role of DC in immune response (8, 9).

Keywords

Dendritic Cell Thymidine Kinase Primary Immune Response Professional Antigen Present Cell Thymic Atrophy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Steinman, R.M., B. Gutchinov, M.D. Witmer, and M.C. Nussenzweig. 1983. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J Exp Med 157: 613–627.PubMedCrossRefGoogle Scholar
  2. 2.
    Croft, M., D.D. Duncan, and S.L. Swain. 1992. Response of naive antigen-specific CD4+ T cells in vitro: characteristics and antigen-presenting cell requirements. J Exp Med 176: 1431–1437.PubMedCrossRefGoogle Scholar
  3. 3.
    Young, J.W., and R.M. Steinman. 1990. Dendritic cells stimulate primary human cytolytic lymphocyte responses in the absence of CD4+ helper T cells. J Exp Med 171, no. 4: 1315–1332.PubMedCrossRefGoogle Scholar
  4. 4.
    Crowley, M., K. Inaba, and R.M. Steinman. 1990. Dendritic cells are the principal cells in mouse spleen bearing immunogenic fragments of foreign proteins. J Exp Med 172, no. 1: 383–386.PubMedCrossRefGoogle Scholar
  5. 5.
    Guéry, J.-C., F. Ria, and L. Adorini. 1996. Dendritic cells but not B cells present antigenic complexes to class II-restricted T cells after administration of protein in adjuvant. J Exp Med 183: 751–757.PubMedCrossRefGoogle Scholar
  6. 6.
    Ronchese, F., and B. Hausmann. 1993. B lymphocytes in vivo fail to prime naive T cells but can stimulate antigen-experienced T lymphocytes. J Exp Med 177, no. 3: 679–690.PubMedCrossRefGoogle Scholar
  7. 7.
    Epstein, M.M., F. Di Rosa, D. Jankovic, A. Sher, and P. Matzinger. 1995. Sccessful T cell priming in B cell-deficient mice. J Exp Med 182: 915–922.PubMedCrossRefGoogle Scholar
  8. 8.
    Burkly, L., C. Hession, L. Ogata, C. Reilly, L.A. Marconi, D. Olson, R. Tizard, R. Cate, and D. Lo. 1995. Expression of relB is required for the development of thymic medulla and dendritic cells. Nature 373, no. 6514: 531–536.PubMedCrossRefGoogle Scholar
  9. 9.
    Weih, F., D. Carrasco, S.K. Durham, D.S. Barton, C.A. Rizzo, R.P. Ryseck, S.A. Lira, and R. Bravo. 1995. Multiorgan inflammation and hematopoietic abnormalities in mice with a targeted disruption of relB, a member of the NF-kB/Rel family. Cell 80: 331–340.PubMedCrossRefGoogle Scholar
  10. 10.
    Salomon, B., R. Lorès, C. Pioche, P. Racz, J. Jami, and D. Klatzmann. 1994. Conditional ablation of dendritic cells in transgenic mice. J Immunol 152: 537–548.PubMedGoogle Scholar
  11. 11.
    St. Clair, M.H., C.U. Lambe, and P.A. Furman. 1987. Inhibition by ganciclovir of cell growth and DNA synthesis of cells biochemically transformed with herpesvirus genetic information. Antimicrobial Agents and Chemotherapy 31: 844–849.CrossRefGoogle Scholar
  12. 12.
    Leonard, J. J.S. Khillan, H.E. Gendelman, A. Adachi, S. Lorenzo, H. Westphal, M.A. Martin, and M.S. Meltzer. 1989. The human immunodeficiency virus long terminal repeat is preferentially expressed in Langerhans cells in transgenic mice. AIDS Res and Hum Retrovir 5, no. 4:421–430.Google Scholar
  13. 13.
    Carrasco, D., R.P. Ryseck, and R. Bravo. 1993. Expression of relB transcripts during lymphoid organ development: specific expression in dendritic antigen-presenting cells. Development 118: 1221–1231.PubMedGoogle Scholar
  14. 14.
    Granelli-Piperno, A., M. Pope, K. Inaba, and R.M. Steinman. 1995. Coexpression of NF-kB/Rel and Spl transcription factors in human immunodeficiency virus 1-induced, dendritic cell-T-cell syncitia. Proc Nall Acad Sci USA 92: 10944–10948.CrossRefGoogle Scholar
  15. 15.
    Salomon, B., C. Pioche, P. Lores, J. Jami, R. Racz, and D. Klatzmann. 1995. Conditional ablation of dendritic cells in mice: comparison of two animal models. Adv Exp Med Bio1378:485–7.Google Scholar
  16. 16.
    Cassell, D.J., and R.H. Schwartz. 1994. A quantitative analysis of antigen-presenting cell function: activated B cells stimulate naive CD4 T cells but are inferior to dendritic cells in providing costimulation. J Exp Med 180, no. 5: 1829–1840.PubMedCrossRefGoogle Scholar
  17. 17.
    Croft, M., L.M. Bradley, and S.L. Swain. 1994. Naive versus memory CD4 T cell response to antigen. Memory cells are less dependent on accessory cell costimulation and can respond to many antigen-presenting cell types including resting B cells. J Immunol 152, no. 6: 2675–2685.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Benoît Salomon
    • 1
  • Pieter J. M. Leenen
    • 2
  • David Klatzmann
    • 1
  1. 1.Laboratoire de Biologie et Thérapeutique des Pathologies Immunitaires CNRS ERS 107, CERVIHôpital de la Pitié-SalpêtrièreParisFrance
  2. 2.Department of ImmunologyErasmus UniversityRotterdamThe Netherlands

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