Differential mRNA Expression in Untreated and TNF-α Elicited Murine Dendritic Cells Precursors

  • Patrizia Rovere
  • Jeannine Trucy
  • Valérie S. Zimmermann
  • Samuel Granjeaud
  • Dominique Rocha
  • Catherine Nguyen
  • Paola Ricciardi-Castagnoli
  • Bertrand R. Jordan
  • Jean Davoust
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)

Summary

We have compared the pattern of gene expression in long term cultured precursor dendritic cells (DC), either untreated (immature) or cultured for two days in the presence of recombinant murine (rm)-TNFα1 (mature). The hybridization signature of complex cDNA probes prepared from total RNA extracted from immature and mature DC were analyzed using a mouse thymic cDNA library, gridded on high density filters. For each clone spotted on the filters, we have measured using an imaging plate device the hybridization signals of the complex probe obtained from immature or mature DC. Comparative analysis of these values allowed us to identify differentially expressed gene products. Our goal is to identify a new set of genes induced or repressed during DC maturation elicited by rmTNFα treatment.

Keywords

Dritic Cell Maturation Immature Dritic Cell Complex Probe Mature Dritic Cell Dritic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Patrizia Rovere
    • 1
  • Jeannine Trucy
    • 1
  • Valérie S. Zimmermann
    • 1
  • Samuel Granjeaud
    • 1
  • Dominique Rocha
    • 1
  • Catherine Nguyen
    • 1
  • Paola Ricciardi-Castagnoli
    • 2
  • Bertrand R. Jordan
    • 1
  • Jean Davoust
    • 1
  1. 1.Centre d’Immunologie INSERM-CNRS de Marseille-LuminyMarseilleFrance
  2. 2.CNR Cellular and Molecular Pharmacology CenterMilanoItaly

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