Subtractive cDNA Cloning

A New Approach to Understanding Dendritic Cell Biology
  • Kiyoshi Ariizumi
  • Paul R. Bergstresser
  • Akira Takashima
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)


Dendritic cells (DC) represent a specific subset of leukocytes that play a critical role in the initiation of T, cell-mediated immune responses. Recently, we have established, from the epidermis of BALB/c mice, long-term DC lines, termed the “XS series”, which resemble resident epidermal DC (i.e., Langerhans cells) in many respects, including their morphology, surface phenotype, antigen presenting capacity, and cytokine and cytokine receptor mRNA profiles (l–3). Taking advantage of these stable lines, we sought to study unique attributes of DC at a molecular level by identifying molecules that they express selectively. For this aim, we employed a subtractive cDNA cloning strategy in which cDNA prepared from the XS52 DC line was subtracted with mRNA isolated from the i774 macrophage line. In this brief communication, we outline our methods as well as the identities of cDNAs isolated in this manner.


Slot Blotting Colony Hybridization Macrophage Line J774 Macrophage Differential Hybridization 
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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Kiyoshi Ariizumi
    • 1
  • Paul R. Bergstresser
    • 1
  • Akira Takashima
    • 1
  1. 1.Department of DermatologyUniversity of Texas Southwestern Medical CenterDallasUSA

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