Summary
The cellular requirements for generating potent human CD8+ CTLs to influenza A virus in vitro have been defined. Furthermore, we have developed improved methods for generating large numbers of DCs from non-proliferating progenitors. These developments have enabled the design of new strategies to elicit CTLs in vivo. For example, together with IL-12, antigen-pulsed DCs may be a useful approach for boosting CTL responses against infectious agents and malignancies. Our results also reopen the potential use of inactivated virus preparations as immunogens for CTL responses.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Bibliography
Bhardwaj, N., A. Bender, N. Gonzalez, L. K. Bui, M. C. Garrett, and R. M. Steinman. 1994. Influenza virus-infected dendritic cells stimulate strong proliferative and cytolytic responses from human CD8+ T cells. J. Clin. Invest. 94: 797.
Bender, A., L. K. Bui, M. A. V. Feldman, M. Larsson, and N. Bhardwaj. 1995. Inactivated influenza virus, when presented on dendritic cells, elicits human CD8+ cytolytic T cell responses. J. Exp. Med. 182: 1663.
Trinchieri, G. 1995. Interleukin-12: a proinflammatory cytokine with immunoregulatory functions that bridge innate resistance and antigen-specific adaptive immunity. Annu. Rev. Immunol. 13: 251.
Trinchieri, G. 1994. Interleukin-12: A cytokine produced by antigen-presenting cells with immunoregulatory functions in the generation of T-helper cells type 1 and cytotoxic lymphocytes. Blood 84: 4008.
Scharton, T. M. and P. Scott. 1993. Natural killer cells are a source of interferon gamma that drives differentiation of CD4+ T cell subsets and induces early resistance to Leishmania major in mice. J. Exp. Med. 178: 567.
Chehimi, J., N. M. Valiante, A. D’Andrea, M. Rengaraju, Z. Rosado, M. Koyayashi, B. Perussia, S. F. Wolf, S. E. Starr, and G. Trinchieri. 1993. Enhancing effect of natural killer cell stimulatory factor [NKSF/interleukin-12] on cell-mediated cytotoxicity against tumor-derived and virus-infected cells. Eue J. Immunol. 23: 1826.
Chan, S. H., B. Perussia, J. W. Gupta, M. Kobayashi, M. Pospisil, H. A. Young, S. F. Wolf, D. Young, S. C. Clark, and G. Trinchieri. 1991. Induction of interferon y production by natural killer cell stimulatory factor: Characterization of the responder cells and synergy with other inducers. J. Exp. Med. 173: 869.
Kobayashi, M., L. Fitz, M. Ryan, R. M. Hewick, S. C. Clark, S. Chan, R. Loudon, F. Sherman, B. Perussia, and G. Trinchieri. 1989. Identification and purification of natural killer cell stimulatory factor [NKSF], a cytokine with multiple biologic effects on human lymphocytes. J. Exp. Med. 170: 827.
Gately, M. K., B. B. Desai, A. G. Wolitzky, P. M. Quinn, C. M. Dwyer, F. J. Podlaski, P. C. Familletti, F. Sinigaglia, R. Chizonnite, U. Gubler, and A. S. Stern. 1991. Regulation of human lymphocyte proliferation by a heterodimeric cytokine, IL-12 [cytotoxic lymphocyte maturation factor]. J. Immunol. 147: 874.
Seder, R. A. and W. E. Paul. 1994. Acquisition of lymphokine-producing phenotype by CD4+ T cells. Annu. Rev. Immunol. 12: 635.
Gately, M. K., A. G. Wolitzky, P. M. Quinn, and R. Chizzonite. 1992. Regulation of human cytolytic lymphocyte responses by interleukin-12. Cell. Immunol. 143: 127.
Chouaib, S., J. Chehimi, L. Bani, N. Genetet, T. Tursz, F. Gay, G. Trinchieri, and F. Mami-Chouaib. 1994. Interleukin 12 induces the differentiation of major histocompatibility complex class I-primed cytotoxic T-lymphocyte precurosrs into allospecific cytotoxic effectors. Proc. Natl. Acad. Sci. USA 91: 12659.
Bloom, E. T. and J. A. Horvath. 1994. Cellular and molecular mechanisms of the IL-l2-induced increase in allospecific murine cytolytic T cell activity. Implications for the age-related decline in CTL. J. Immunol. 152: 4242.
Mehrotra, P. T., D. Wu, J. A. Crim, H. S. Mostowski, and J. P. Siegel. 1993. Effects of IL-12 on the generation of cytotoxic activity in human CD8+ T lymphocytes. J. Immunol. 151: 2444.
Macatonia, S. E., N. A. Hosken, M. Litton, P. Vieira, C. Hsieh, J. A. Culpepper, M. Wysocka, G. Trinchieri, K. M. Murphy, and A. O’Garra. 1995. Dendritic cells produce IL-12 and direct the development of Th I cells from naive CD4+ T cells. J. Immunol. 154: 5071.
Bhardwaj, N., R. A. Seder, A. Reddy, and M. V. Feldman. 1996. IL-12 in conjunction with dendritic cells enhances anti-viral, CD8+ CTL responses in vitro. J. Clin. Invest. 98: 715.
Bender, A., M. Sapp, G. Schuler, R. M. Steinman, and N. Bhardwaj. 1996. Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121.
Romani, N., D. Reider, M. Heuer, S. Ebner, B. Eibl, D. Niederwieser, and G. Schuler. 1996. Generation of mature dendritic cells from human blood: An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media New York
About this chapter
Cite this chapter
Bender, A. et al. (1997). Dendritic Cells as Immunogens for Human CTL Responses. In: Ricciardi-Castagnoli, P. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 417. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9966-8_62
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9966-8_62
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9968-2
Online ISBN: 978-1-4757-9966-8
eBook Packages: Springer Book Archive