FACS-Sorted Spleen and Peyer’s Patch Dendritic Cells Induce Different Responses in Th0 Clones

  • Michael P. Everson
  • David G. Lemak
  • Jerry R. Mcghee
  • Kenneth W. Beagley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)


Helper T cells in mice have been divided into two functionally distinct subsets based upon the pattern of cytokines secreted,1,2 with Th type 1 (Th l) cells producing IL-2 and IFN-γ upon activation and predominantly mediating cell-mediated immunity (CMI), and Th type 2 (Th2) cells producing IL-4, IL-5, IL-6, and IL-10 upon activation and primarily mediating humoral immunity by providing T cell help via cytokines for Ig isotype and subclass responses.1–4 For T cell activation, dendritic cells (DC) are the most potent inducers of primary in vivo T cell responses and are the principle in vitro stimulators of naive T cell activation in both mice and humans.5,6 Previous studies showed that DC from different lymphoid tissues could possess similar7 or different8 functions. These studies supported the notion that PP DC induce preferential IgA production (predominantly found at mucosal sites) and SP DC support IgM production by controlling the cytokines produced by T cells in mucosal and systemic tissues, respectively. Recent studies suggest that cytokines present during primary activation of naive T cells play a role in determining the pattern of cytokines produced during subsequent antigenic challenge.9 Perhaps more important to these stimulatory events are the APC or accessory cells (AC) resident in the local tissues that can stimulate initial cytokine secretion in this inductive milieu. In this regard, the present studies have addressed tissue specificity of the AC component of T cell activation using a single type of AC, namely, DC isolated from two different anatomical sites. We asked whether SP DC and PP DC, isolated under identical conditions, induce production of similar levels of T cell-derived cytokines that are involved in regulation of immune responses.


Dendritic Cell Accessory Cell Macrophage Subpopulation Counter Receptor Spleen Dendritic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    T.R. Mosmann and R.L. Coffman, THI and TH2 cells: different patterns of lymphokine secretion lead to different functional properties, Annu. Rev. Immunol. 7: 145 (1989).PubMedCrossRefGoogle Scholar
  2. 2.
    T.R. Mosmann, J.H Schumacher, et al., Diversity of cytokine synthesis and function of mouse CD4’ T cells, Immuno. Rev. 123: 209 (1991).CrossRefGoogle Scholar
  3. 3.
    C. Pfeiffer, J. Murray, J. Madri, and K. Bottomly, Selective activation of Thl - and Th2-like cells in vivo - response to human collagen 1V, Immunol. Rev. 123: 65 (1991).PubMedCrossRefGoogle Scholar
  4. 4.
    S.L. Swain, L.M. Bradley, et al., Helper T-cell subsets: phenotype, function and the role of lymphokines in regulating their development, Immunol. Rev. 123: 115 (1991).PubMedCrossRefGoogle Scholar
  5. 5.
    K. Inaba, J. Metlay, M.T. Crowley, and R.M. Steinman, Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific, MHC-restricted T cells in situ, J. Exp. Med. 172: 631 (1990).PubMedCrossRefGoogle Scholar
  6. 6.
    W.C. Van Voohris, J. Valinsky, et al., Relative efficacy of human monocytes and dendritic cells as accessory cells for T cell replication, J. Exp. Med. 158: 174 (1983).CrossRefGoogle Scholar
  7. 7.
    D.M. Spalding, W.J. Koopman, J.H. Eldridge, J.R. McGhee, and R.M. Steinman, Accessory cells in murine Peyer’s patch.ldentification and enrichment of a functional dendritic cell, J.Exp.Med. 157: 1646 (1983).PubMedCrossRefGoogle Scholar
  8. 8.
    D.M. Spalding and J.A. Griffin, Different pathways of differentiation of pre-B cell lines are induced by dendritic cells and T cells from different lymphoid tissue, Cell 44: 507 (1986).PubMedCrossRefGoogle Scholar
  9. 9.
    C.S. Hsieh, S.E. Macatonia, C.S. Tripp, S.F. Wolf, A. O’Garra, and K.M. Murphy, Development of THI CD4+ T cells through IL-12 produced by Listeria-induced macrophages, Science 260: 547 (1993).PubMedCrossRefGoogle Scholar
  10. 10.
    J.P. Metlay, M.D. Witmer-Pack, et al., The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies, J. Exp. Med. 171: 1753 (1990).PubMedCrossRefGoogle Scholar
  11. 11.
    M.C. Nussenzweig, M.C., R.M. Steinman, M.D. Witmer, and B. Gutchinov, A monoclonal antibody specific for mouse dendritic cells, Proc. Natl. Acad. Sci. USA 79: 161 (1982).PubMedCrossRefGoogle Scholar
  12. 12.
    J.M. Austyn and S. Gordon, F4/80, a monoclonal antibody directed specifically against the mouse macrophage, Eue J. Immunol. 11: 805 (1981).CrossRefGoogle Scholar
  13. 13.
    J.C. Unkeless, Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors, J. Exp. Med. 150: 580 (1979).PubMedCrossRefGoogle Scholar
  14. 14.
    M.P. Everson, D.M. Spalding, and W.J. Koopman, Exquisite sensitivity of dendritic cells to ultraviolet radiation and temperature changes, Transplantation 48: 666 (1989).PubMedGoogle Scholar
  15. 15.
    D.L. Mueller, L. Chiodetti, P.A. Brown, and R.H. Schwartz, Clonal anergy blocks the response to IL-4, as well as the production of IL-2, in dual-producing T helper cell clones, J. Immunol. 147: 4118 (1991).Google Scholar
  16. 16.
    D.S. Reynolds, W.H. Boom, and A.K. Abbas, Inhibition of B lymphocyte activation by interferon-y, J. Immunol. 139: 767 (1987).PubMedGoogle Scholar
  17. 17.
    M.P. Everson, D.M. Spalding, and W.J. Koopman, Enhancement of IL-2-induced T cell proliferation by a novel factor(s) present in murine spleen dendritic cell-T cell culture supernatants, J. Immunol. 142: 1183 (1989).PubMedGoogle Scholar
  18. 18.
    J.H. Schumacher, A. O’Garra, B. Shrader, A. van Kimmenade, M.W. Bond, T.R. Mosmann, and R.L. Coffman, The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent, J. Immunol. 141: 1576 (1988).PubMedGoogle Scholar
  19. 19.
    M.P. Everson, D.S. McDuffie, D.G. Lemak, W.J. Koopman, J.R. McGhee, and K.W. Beagley, Dendritic cells from different tissues induce production of different T-cell cytokine profiles, J.Leuk.Biol. 59: 494–498 (1996).Google Scholar
  20. 20.
    T.T. Hecht, D.L. Longo, and L.A. Matis, The relationship between immune interferon production and proliferation in antigen-specific, MHC-restricted T cell lines and clones, J. Immunol. 131: 1049 (1983).PubMedGoogle Scholar
  21. 21.
    M.P. Everson and D.B. Chandler, Changes in distribution, morphology, and tumor necrosis factor-a secretion of alveolar macrophage subpopulations during the development of bleomycin-induced pulmonary fibrosis, Am. J. Pathol. 140: 503 (1992).PubMedGoogle Scholar
  22. 22.
    C.A. Janeway, Jr., S. Carding, B. Jones, J. Murray, P. Portoles, R. Rasmussen, J. Rojo, K. Saizawa, J. West, and K. Bottomly, CD4+ T cells: specificity and function, Immunol. Rev. 101: 39 (1988).PubMedCrossRefGoogle Scholar
  23. 23.
    J.M. Austyn, R.M. Steinman, D.E. Weinstein, A. Granelli-Piperno, and M.A. Pallidino, Dendritic cells initiate a two-stage mechanism of T lymphocyte proliferation, J. Exp. Med. 157: 1101 (1983).PubMedCrossRefGoogle Scholar
  24. 24.
    S.E. Macatonia, N.A. Hosken, et al., Dendritic cells produce IL-12 and direct the development of Th l cells from naive CD4’ T cells, J. Immunol. 154: 5071 (1995).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Michael P. Everson
    • 1
    • 3
  • David G. Lemak
    • 1
  • Jerry R. Mcghee
    • 2
  • Kenneth W. Beagley
    • 1
    • 2
    • 4
  1. 1.Department of MedicineThe University of Alabama at BirminghamBirminghamUSA
  2. 2.Department of MicrobiologyThe University of Alabama at BirminghamBirminghamUSA
  3. 3.Birmingham Department of Veterans Affairs Medical CenterBirminghamUSA
  4. 4.Discipline of PathologyThe University of NewcastleNewcastleAustralia

Personalised recommendations