FACS-Sorted Spleen and Peyer’s Patch Dendritic Cells Induce Different Responses in Th0 Clones
Helper T cells in mice have been divided into two functionally distinct subsets based upon the pattern of cytokines secreted,1,2 with Th type 1 (Th l) cells producing IL-2 and IFN-γ upon activation and predominantly mediating cell-mediated immunity (CMI), and Th type 2 (Th2) cells producing IL-4, IL-5, IL-6, and IL-10 upon activation and primarily mediating humoral immunity by providing T cell help via cytokines for Ig isotype and subclass responses.1–4 For T cell activation, dendritic cells (DC) are the most potent inducers of primary in vivo T cell responses and are the principle in vitro stimulators of naive T cell activation in both mice and humans.5,6 Previous studies showed that DC from different lymphoid tissues could possess similar7 or different8 functions. These studies supported the notion that PP DC induce preferential IgA production (predominantly found at mucosal sites) and SP DC support IgM production by controlling the cytokines produced by T cells in mucosal and systemic tissues, respectively. Recent studies suggest that cytokines present during primary activation of naive T cells play a role in determining the pattern of cytokines produced during subsequent antigenic challenge.9 Perhaps more important to these stimulatory events are the APC or accessory cells (AC) resident in the local tissues that can stimulate initial cytokine secretion in this inductive milieu. In this regard, the present studies have addressed tissue specificity of the AC component of T cell activation using a single type of AC, namely, DC isolated from two different anatomical sites. We asked whether SP DC and PP DC, isolated under identical conditions, induce production of similar levels of T cell-derived cytokines that are involved in regulation of immune responses.
KeywordsDendritic Cell Accessory Cell Macrophage Subpopulation Counter Receptor Spleen Dendritic Cell
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