Growth and Differentiation of Human Dendritic Cells from CD34+ Progenitors

  • Paul Szabolcs
  • David H. Ciocon
  • Malcolm A. S. Moore
  • James W. Young
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)


Several groups originally established the importance of GM-CSF and TNFα in the generation of dendritic cells from CD34+ precursors (1–3), and most investigators have used cord blood as the starting source. Studies from our laboratories confirmed the essential role of these cytokines in supporting bone marrow CD34+-derived dendritic cell growth and differentiation, and the addition of c-kit-ligand facilitated more detailed phenotypic and functional characterization of the dendritic cell progeny than had previously been possible (4).


Dendritic Cell Cord Blood Human Dendritic Cell Bone Marrow Progenitor Cord Blood CD34 
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  1. 1.
    Caux, C., Dezutter-Dambuyant, C., Schmitt, D., and Banchereau, J. 1992. GM-CSF and TNF-a cooperate in the generation of dendritic Langerhans cells. Nature 360: 258–261.PubMedCrossRefGoogle Scholar
  2. 2.
    Reid, C.D.L., Stackpoole, A., Meager, A., and Tikerpae, J. 1992. Interactions of tumor necrosis factor with granulocyte-macrophage colony-stimulating factor and other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34+ progenitors in human bone marrow. J. Immunol. 149: 2681–2688.PubMedGoogle Scholar
  3. 3.
    Santiago-Schwarz, F., Belilos, E., Diamond, B., and Carsons, S.E. 1992. TNF in combination with GMCSF enhances the differentiation of neonatal cord blood stem cells into dendritic cells and macrophages. J. Leuk. Biol. 52: 274–281.Google Scholar
  4. 4.
    Szabolcs, P., Moore, M.A.S., and Young, J.W. 1995. Expansion of immunostimulatory dendritic cells among the myeloid progeny of human CD34` bone marrow precursors cultured with c-kit ligand, granulocyte-macrophage colony-stimulating factor, and TNF-a. J. Immunol. 154:5851–5861.Google Scholar
  5. 5.
    Caux, C.,Vanbervliet, B.. Massacrier, C., Durand, I., and Banchereau, J. 1996. Interleukin-3 cooperates with tumor necrosis factor alpha for the development of human dendritic/langerhans cells from cord blood CD34+ hematopietic progenitor cells. Blood 87:2376–2385.Google Scholar
  6. 6.
    Szabolcs, P., Avigan, D., Gezelter, S., Ciocon, D.H., Moore, M.A.S., Steinman, R.M., and Young, J.W. 1996. Dendritic cells and macrophages can mature independently from a human bone marrow-derived, post-CFU intermediate. Blood 87: 4520–4530.PubMedGoogle Scholar
  7. 7.
    Caux, C., Vanbervliet, B., Massacrier, C., Dezutter-Dambuyant, C., de Saint-Vis, B., Jacquet, C.. Yoneda, K., Imamura, S., Schmitt, D., and Banchereau, J. 1996. CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF+ TNF alpha. J. Exp. Med. 184: 695–706.Google Scholar
  8. 8.
    Inaba, K., Inaba, M., Deguchi, M., Hagi, K., Yasumizu, R., Ikehara, S., Muramatsu, S., and Steinman, R.M. 1993. Granulocytes, macrophages, and dendritic cells arise from a common major histocompatibility complex class II-negative progenitor in mouse bone marrow. Proc. Natl. Acad. Sci. USA 90: 3038–3042.PubMedCrossRefGoogle Scholar
  9. 9.
    Young, J.W., Szabolcs, P., and Moore, M.A.S. 1995. Identification of dendritic cell colony-forming units among normal CD34 bone marrow progenitors that are expanded by c-kit-ligand and yield pure dendritic cell colonies in the presence of granulocyte/macrophage colony-stimulating factor and tumor necrosis factor a. J. Exp. Med. 182:1 1 1 1–1 120.Google Scholar
  10. 10.
    Zhou, L.-J. Tedder, T.F. 1995. Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily. J. Immunol. 154: 3821–3835.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Paul Szabolcs
    • 1
    • 2
  • David H. Ciocon
    • 1
  • Malcolm A. S. Moore
    • 3
  • James W. Young
    • 1
    • 4
  1. 1.Laboratory of Cellular Physiology and ImmunologyThe Rockefeller UniversityUSA
  2. 2.Bone Marrow Transplantation Service Department of PediatricsMemorial Sloan-Kettering Cancer CenterUSA
  3. 3.James Ewing Laboratory of Developmental Hematopoiesis, Cell Biology and Genetics ProgramSloan-Kettering Institute for Cancer ResearchUSA
  4. 4.Allogeneic Bone Marrow Transplantation and Clinical Immunology Services Division of Hematologic Oncology, Department of MedicineMemorial Sloan-Kettering Cancer CenterNew YorkUSA

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