The Influence of Collagen, Fibronectin, and Laminin on the Maturation of Dendritic Cell Progenitors Propagated from Normal or Flt3-Ligand-Treated Mouse Liver

  • Maureen L. Drakes
  • Lina Lu
  • Hilary J. Mckenna
  • Angus W. Thomson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)


Dendritic cell (DC) progenitors can be propagated from normal mouse liver non-parenchymal cell suspensions in the presence of granulocyte/macrophage colony stimulating factor (GM-CSF). These cells strongly express CD45, CD11b, heat stable antigen and CD44 cell surface markers. They exhibit moderate to low levels of expression of mouse DC-restricted markers (DEC-205, 33D1 and N418 [CD11c]) as well as low levels of MHC class II and B7–2 molecules, features characteristic of “immature” mouse DC.1,2,3 We have shown previously that these cells can be induced to express a mature DC phenotype and function (allostimulatory activity) following exposure to type-I collagen1, a molecule that is spatially associated with DC in the portal triads of normal liver.4–7 Here we report that these liver DC progenitors can also be induced to maturation in the presence of other extracellular matrix (ECM) proteins, namely fibronectin and laminin. Fibronectin is present along hepatic sinusoids of the liver, while laminin is localized on basement membranes along bile duct systems and blood vessels.4


Flt3 Ligand CD44 Cell Surface Marker Bile Duct System Mouse Dendritic Cell Uncoated Plate 
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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Maureen L. Drakes
    • 1
    • 2
  • Lina Lu
    • 1
    • 2
  • Hilary J. Mckenna
    • 4
  • Angus W. Thomson
    • 1
    • 2
    • 3
  1. 1.Thomas E. Starzl Transplantation InstituteUniversity of PittsburghUSA
  2. 2.Department of SurgeryUniversity of PittsburghUSA
  3. 3.Department Molecular Genetics and BiochemistryUniversity of PittsburghUSA
  4. 4.Immunex Research Development CorporationSeattleUSA

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