Mouse Spleen Dendritic Cells
Recent advances in the field have made increasingly clear that dendritic cells (DC) are heterogeneous in function, localization and developmental derivation1. In the spleen, two populations of DC have been demonstrated which show differences in location and immunophenotype2,3. Classic interdigitating cells (IDC) are located in the T cell areas of the splenic white pulp, whereas a less well characterized population of DC is situated in patches just outside the marginal zone. The developmental relationship between these two populations, however, is unclear. In the present study, we wished to explore the functional and phenotypic characteristics of splenic DC, and especially of the marginal DC population. The relevance of this aim was indicated by the observation that the majority of freshly isolated splenic DC is derived from this marginal population3. More specifically, we approached the question whether marginal DC are capable of endocytosing particulate antigens in vivo, since the spleen is important in the initiation of immune responses against circulating antigens. In immature developmental stages, DC may show uptake of solutes by means of macropinocytosis, but their phagocytic capacities are thought to be limited (discussed in ref. 4).
KeywordsDendritic Cell White Pulp Macrophage Marker Phagocytic Capacity Dendritic Cell Population
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- 1.Stingl G, Bergstresser PR. Dendritic cells: a major story unfolds. Immunol.Today 16: 330–333.Google Scholar
- 4.Steinman RM, Swanson J. 1995. The endocytic activity of dendritic cells. J.Exp. Med. 182: 283–288.Google Scholar
- 8.Leenen PJM, Kraal G. Dijkstra CD. 1996. Markers of rodent myeloid cells. In: Immunology Methods Manual, Lefkovits 1 (ed.), section 34.2, Academic Press, New York, pp. 2467–2495.Google Scholar
- 1.Lutz MB, Allmann CU, Girolomoni G. Ricciardi-Castagnoli P. 1996. Different cytokines regulate antigen uptake and presentation of a precursor dendritic cell line. Eur.J.Immunol. 26: 586–594.Google Scholar