Advertisement

Mechanisms of Injury and Protection in Living Cells and Tissues at Low Temperatures

  • J. Farrant
Part of the The International Cryogenics Monograph Series book series (INCMS)

Abstract

There is an apparent contradiction between the use of very low temperatures to store isolated cells and tissues for long periods and the experimental finding that living cells are usually destroyed by freezing. It is in understanding ways of overcoming the damage caused by freezing that progress towards the very long-term storage of cells has been achieved.

Keywords

Cool Rate Sodium Chloride Freezing Point High Solute Concentration Protective Compound 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Gehenio, P. M., Rapatz, G. L., and Luyet, B., “Effects of freezing velocities in causing or preventing haemolysis,” Biodynamica, 9, 77–82 (1963).Google Scholar
  2. 2.
    Levitt, J., “A sulfhydryl-disulfide hypothesis of frost injury and resistance in plants,” J. Theoret. Biol., 3, 355–391 (1962).CrossRefGoogle Scholar
  3. 3.
    Lovelock, J. E., “The haemolysis of human red blood-cells by freezing and thawing,” Biochim. Biophys. Acta, 10, 414–426 (1953).CrossRefGoogle Scholar
  4. 4.
    Lovelock, J. E., “The mechanism of the protective action of glycerol against haemolysis by freezing and thawing,” Biochim. Biophys. Acta, 11, 28–36 (1953).CrossRefGoogle Scholar
  5. 5.
    Mazur, P., “Kinetics of water loss from cells at subzero temperatures and the likelihood of intracellular freezing,” J. Gen. Physiol., 47, 347–369 (1963).CrossRefGoogle Scholar
  6. 6.
    Stephenson, J. L., “Fundamental physical problems in the freezing and drying of biological materials,” in (Parkes, A. S. and Smith, A. U., eds.) Recent Research in Freezing and Drying, pp. 121–145, Blackwell, Oxford (1960).Google Scholar
  7. 7.
    Polge, C., Smith, A. U., and Parkes, A. S., “Revival of spermatozoa after vitrification and dehydration at low temperatures,” Nature (London), 165, 666 (1949).CrossRefGoogle Scholar
  8. 8.
    Farrant, J., “The preservation of living cells, tissues, and organs at low temperatures: some underlying principles,” Lab. Pract., 15, 402–404 (1966).Google Scholar
  9. 9.
    Miner, C. S. and Dalton, N. N. (eds.) Glycerol, ACS #117, Reinhold Book Corporation, New York (1953).Google Scholar
  10. 10.
    Huggins, C. E., “Prevention of haemolysis of large volumes of red blood cells slowly frozen and thawed in the presence of dimethyl sulphoxide,” Transfusion (Philadelphia) 3, 483–493 (1963).CrossRefGoogle Scholar
  11. 11.
    Farrant, J., “Mechanisms of cell damage during freezing and thawing and its prevention,” Nature (London) 205, 1284–1287 (1965).CrossRefGoogle Scholar
  12. 12.
    Rapatz, G. and Luyet, B., “Effects of cooling rates on the preservation of erythrocytes in frozen blood containing various protective agents,” Biodynamica, 9, 333–350 (1965).Google Scholar

Copyright information

© Springer Science+Business Media New York 1970

Authors and Affiliations

  • J. Farrant
    • 1
  1. 1.Clinical Research Centre LaboratoriesNational Institute for Medical ResearchLondonEngland

Personalised recommendations