Computational Aspects of Multinuclear NMR Spectroscopy of Proteins at NMRFAM

  • John L. Markley
  • Prashanth Darba
  • Jasna Fejzo
  • Andrzej M. Krezel
  • Slobodan Macura
  • Charles W. McNemar
  • Ed S. Mooberry
  • Beverly R. Seavey
  • William M. Westler
  • Zsolt Zolnai
Chapter
Part of the NATO ASI Series book series (NSSA, volume 225)

Abstract

In recent studies, we have explored various strategies for using isotope labeling in conjunction with 1D, 2D, and 3D NMR spectroscopy to obtain information about how proteins work. With larger proteins, a concerted approach to the assignment of protein spectra that makes use of all available information about through-bond and through-space connectivities appears to provide the most reliable results. We summarize here various ways in which we have been using computers to process and analyze data and to derive information about protein structure and dynamics. We also describe in brief our protein NMR database project.

Keywords

Spin System Cross Peak Distance Constraint Array Processor Cross Relaxation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • John L. Markley
    • 1
    • 2
  • Prashanth Darba
    • 1
    • 2
  • Jasna Fejzo
    • 1
    • 2
  • Andrzej M. Krezel
    • 1
    • 3
  • Slobodan Macura
    • 1
    • 3
  • Charles W. McNemar
    • 1
    • 4
  • Ed S. Mooberry
    • 1
    • 4
  • Beverly R. Seavey
    • 1
    • 4
  • William M. Westler
    • 1
    • 4
  • Zsolt Zolnai
    • 1
    • 4
  1. 1.National Magnetic Resonance Facility at Madison and Biochemistry Department, College of Agricultural and Life SciencesUniversity of WisconsinMadisonUSA
  2. 2.Institute of Physical ChemistryUniversity of BelgradeBeogradYugoslavia
  3. 3.Department of Biochemistry and Molecular BiologyMayo FoundationRochesterUSA
  4. 4.Mathematical InstituteBeogradYugoslavia

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