Structural Interpretation of NMR Data in the Presence of Motion

  • J. H. Prestegard
  • Yangmee Kim
Part of the NATO ASI Series book series (NSSA, volume 225)


Proteins exhibit a variety of internal motions that are, in many cases, necessary for function. Yet, most approaches used to convert NMR data into a three dimensional structure assume a rigid model in translating cross-relaxation data into proton-proton distance constraints. For some types of motion this can lead to substantial errors in the final structure determined. Means of recognizing the presence of significant internal motions and means of minimizing the impact on the structures determined are discussed. Data on a small protein important in fatty acid biosynthesis, acyl carrier protein from spinach, are used to illustrate the procedures.


Cross Peak Internal Motion Acyl Carrier Protein Distance Constraint Rigid Model 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • J. H. Prestegard
    • 1
  • Yangmee Kim
    • 1
  1. 1.Department of ChemistryYale UniversityNew HavenUSA

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