Laboratory Methods and Test Parameters

  • Jessica H. Lewis


Over the 30 years during which these studies were done, the laboratory methods have changed greatly. At first, all were manual—eyeball techniques. Currently, most clotting—counting tests are automated. Despite these changes in technique, the reagents used and the results obtained have changed very little, and comparisons between old and new test values are quite valid. On the other hand, comparisons between species are fraught with technical difficulties. It has long been recognized that many protein lipid interactions are species-specific.


Protein Lipid Interaction Calcium Phosphorus Monochloroacetic Acid Chloride Carbon Clot Time Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Suggested Readings

  1. Amiral, J., Adalbert, B., and Adam, M., 1984, Application of enzyme immunoassays to coagulation testing, Clin. Chem. 30: 1512.PubMedGoogle Scholar
  2. Bang, N. V., Beller, F. K., Deutsch, E., and Mammen, E. F., eds., 1971, Thrombosis and Bleeding Disorders: Theory and Methods. Academic Press, New York.Google Scholar
  3. Born, G. V. R., and Hardisty, R. M., 1976, Platelets, in: Human Blood Coagulation, Haemostasis and Thrombosis, 2nd ed. ( R. Biggs, ed.), pp. 168–201, Blackwell, London.Google Scholar
  4. Boyer, C., Rothschild, C., Wolf, M., Amiral, J., Meyer, D., and Larrieu, M. J., 1984, A new method for the estimation of protein C by ELISA, Thromb. Res. 36: 579.PubMedCrossRefGoogle Scholar
  5. Brandt, J. T., and Triplett, D. A., 1981, Am. J. Clin. Pathol. 76 (Suppl.): 530.PubMedGoogle Scholar
  6. Coleman, R. W., Hirsh, J., Marder, V. J., and Salzman, E. W., 1987, Hemostasis and Thrombosis, 2nd ed., J. B. Lippincott, Philadelphia.Google Scholar
  7. Comp, P. C., Nixon, R., and Esmon, C. T., 1984, Determinations of functional levels of protein C, an antithrombotic protein, using thrombin/thrombomodulin complex, Blood 63: 16.Google Scholar
  8. Day, H. J., and Holmsen, H., 1972, Laboratory tests of platelet function, Ann. Clin. Lab. Sci. 2: 63.PubMedGoogle Scholar
  9. Esmon, C. T., 1983, Protein C biochemistry, physiology and clinical implications, Blood 62: 1155.PubMedGoogle Scholar
  10. Esmon, C. T., and Esmon, N. L., 1984, Protein C activation, Semin. Thromb. Hemost. 10: 122.PubMedCrossRefGoogle Scholar
  11. Gallimore, M. J., Amundsen, E., Assen, A. O., Laarsbraten, M., Lyngass, K., and Svendsen, L., 1979, Studies on plasma antiplasmin activity using a new specific chromogenic tripeptide substrate, Thromb. Res. 14: 51.PubMedCrossRefGoogle Scholar
  12. Gaspar, H., 1978, Collagen—platelet interaction, Thromb. Haemost., Supplement No. 63.Google Scholar
  13. Kasper, C. K., .,1975, A more uniform measurement of factor VIII inhibitors, Thromb. Haemat. 34:869.Google Scholar
  14. Lewis, J. H., 1961, Coagulation defects, J. Am. Med. Assoc. 178: 1014.CrossRefGoogle Scholar
  15. Lewis, J. H., Iammarino, R. M., Spero, J. A., and Hasiba, U., 1978, Antithrombin Pittsburgh: An al-anti trypsin variant causing hemorrhagic disease, Blood 51: 129.PubMedGoogle Scholar
  16. Miale, J. B., 1977, Laboratory Medicine: Hematology, 5th ed., C. V. Mosby, St. Louis.Google Scholar
  17. Miale, J. B., and LaFond, D., 1969, Prothrombin time standardization, Am. J. Clin. Pathol. 52: 154.PubMedGoogle Scholar
  18. Murano, G., and Bick, R. L., 1980, Basic concepts of hemostasis and thrombosis, in: Platelet Disorders ( D. Triplett, ed.), pp. 101, CRC Press, Boca Raton.Google Scholar
  19. Naito, K., and Aoki, N., 1978, Assay of alpha-2 plasmin inhibitor activity by means of a plasmin specific tripeptide substrate, Thromb. Res. 12: 1147.PubMedCrossRefGoogle Scholar
  20. Odegard, O. R., Lie, M., and Abilgaard, U., 1975, Heparin cofactor activity measured with an amidolytic method, Thromb. Res. 6: 287.PubMedCrossRefGoogle Scholar
  21. Olson, J. D., Brockway, W. J., Fass, D. N., Magnuson, M. A., and Bowie, E. J. W., 1975, Evaluation of ristocetin—Willebrand factor assay and ristocetin-induced platelet aggregation, Am. J. Clin. Pathol. 63: 210.PubMedGoogle Scholar
  22. Owen, W. G., and Esmon, C. T., 1981, Functional properties of an endothelial cell cofactor for thrombin catalyzed activation of protein C, J. Biol. Chem. 256: 5532.PubMedGoogle Scholar
  23. Penner, J. A., 1974, Experience with a thrombin clotting time assay for measuring heparin activity, Am. J. Clin. Pathol. 61: 645.PubMedGoogle Scholar
  24. Pesce, A. J., Ford, D. J., and Gaizutis, M. A., 1978, Qualitative and quantitative aspects of immunoassays. Scand. J. Immunol. 8 (Suppl.): 1.CrossRefGoogle Scholar
  25. Ramsey, R., and Evatt, B. L., 1979, Rapid assay for von Willebrand factor activity using formalinfixed platelets and microtitration technic, Am. J. Clin. Pathol. 72: 996.PubMedGoogle Scholar
  26. Reddy, K. N. N., and Markus, G. J., 1972, Mechanism of activation of human plasminogen by streptokinase: Presence of active center in streptokinase—plasminogen complex, J. Biol. Chem. 247: 1683.PubMedGoogle Scholar
  27. Rosenberg, R. D., 1975, Actions and interactions of antithrombin and heparin, N. Engl. J. Med. 292: 146.PubMedCrossRefGoogle Scholar
  28. Salem, A., and Cieslica, R., 1976, Comparison of the Coag-a-mate dual channel with the Fibrome-ter, Am. J. Med. Technol. 42: 39.Google Scholar
  29. Samama, M., Schleguel, N., Cazenave, B., Horellou, M. M., Conrad, J., Castel, M., and Douenias, R., 1980, Alpha-2-antiplasmin assay: Amidolytic and immunological method. Critical evaluation results in a clinical material, in: Synthetic Substrates in Clinical Blood Coagulation Assays ( H. R. Lijnen, D. Collen, and M. Verstraete, eds.), pp. 93–99, Martinus Nijhoff, The Hague.CrossRefGoogle Scholar
  30. Stenflo, J., 1984, Structure and function of protein C, Semin. Thromb. Hemost. 10: 109.PubMedCrossRefGoogle Scholar
  31. Stenflo, J., and Jonsson, M., 1979, Protein S, a new vitamin K—dependent protein from bovine plasma, FEBS Lett. 101: 377.PubMedCrossRefGoogle Scholar
  32. Stocker, K., and Meier, J., 1985, Thrombin-like snake venom enzymes, paper presented at the Symposium on Animal Venoms and Haemostasis, San Diego, July 20–21.Google Scholar
  33. Suzuki, K., Nishioka, J., and Hashimoto, S., 1983, Regulation of activated protein C by thrombin-modified protein S, J. Biochem. 94: 699.PubMedGoogle Scholar
  34. Svendsen, L., 1981, Synthetic peptide substrate: A critical perspective, in: Perspective in Hemostasis (J. Fareed, H. L. Messmore, J. W. Fenton II, and K. M. Brinkhous, eds.), pp. 343–353, Pergamon Press, New York.Google Scholar
  35. Teger-Nilson, A. C, Friberger, P., and Gyzander, E., 1977, Determination of a new rapid plasmin inhibitor in human blood by means of a plasmin specific tripeptide substrate, Scand. J. Clin. Lab. Invest. 37: 403.CrossRefGoogle Scholar
  36. Thomson, J. M. (ed.), 1980, Blood and Coagulation and Haemostasis, Churchill Livingstone, London.Google Scholar
  37. Triplett, D. A., Harms, C. S., Newhouse, P., and Clark, C., 1978, Platelet Function: Laboratory Evaluation and Clinical Application, American Society of Clinical Pathologists, Chicago.Google Scholar
  38. Walker, F. J., 1980, Regulation of activated protein C by a new protein, J. Biol. Chem. 255: 1260.Google Scholar
  39. Walker, F. J., 1984, Protein S and the regulation of activated protein C, Semin. Thromb. Hemost. 10: 131.PubMedCrossRefGoogle Scholar
  40. Weiss, P., Soff, G. A., Halkin, H., and Seligsohn, U., 1987, Decline of proteins C and S and factors II, VII, IX and X during the initiation of warfarin therapy, Thromb. Res. 45: 783.PubMedCrossRefGoogle Scholar
  41. Wohl, R. C., Sinio, L., and Robbins, K. C., 1982, Methods for studying fibrinolytic pathway components in human plasma, Thromb. Res. 27: 520.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Jessica H. Lewis
    • 1
  1. 1.University of Pittsburgh School of Medicine and Central Blood BankPittsburghUSA

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