Skip to main content

Solvent Production

  • Chapter
Clostridia

Part of the book series: Biotechnology Handbooks ((BTHA,volume 3))

Abstract

Fermentation processes using anaerobic microorganisms provide a potential route for the conversion of plant biomass and wastes from agriculture and industry to chemical feedstocks and fuels (Wiegel, 1980; Zeikus, 1980; Rogers, 1984). However, only a few industrial fermentation processes exist which utilize single species of anaerobic microorganisms for the production of acids and alcohols. The need to develop novel processes which are efficient and less energy-intensive, and which can compete economically with processes employing chemical synthesis, represents a major challenge for the biotechnology industry. Of the existing industrial fermentation processes, the production of bioethanol has achieved the greatest success and attracted the most attention. The only large-scale industrial fermentation utilizing anaerobic bacteria which has made a significant contribution to the production of chemical feedstocks is the acetone/butanol/ethanol fermentation (ABE fermentation) using Clostridium acetobutylicum strains (Jones and Woods, 1986). The ABE fermentation was the major route used for the production of these solvents during the first part of the century and up until the early 1960s was able to compete successfully with synthetic processes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Adler, H. I., and Crow, W., 1987, A technique for predicting the solvent-producing ability of Clostridium acetobutylicum. Appl. Env. Microbiol. 53:2496–2499.

    CAS  Google Scholar 

  • Afschar, A. S., Biebl, H., Schaller, K., and Schugerl, K., 1985, Production of acetone and butanol by Clostridium acetobutylicum in continuous culture with cell recycle, Appl. Microbiol. Biotechnol. 22:394–398.

    CAS  Google Scholar 

  • Afschar, A. S., Schaller, K., and Schurgerl, K., 1986, Continuous production of acetone and butanol with shear-activated Clostridium acetobutylicum, Appl. Microbiol. Biotechnol. 23:315–321.

    CAS  Google Scholar 

  • Allcock, E. R., and Woods, D. R., 1981, Carboxymethyl cellulase and cellobiase production by Clostridium acetobutylicum in an industrial fermentation medium, Appl. Env. Microbiol. 41 (2):539–541.

    CAS  Google Scholar 

  • Andersch, W., Bahl, H., and Gottschalk, G., 1982, Acetone-butanol production by Clostridium acetobutylicum in an ammonium-limited chemostat at low pH values, Biotechnol. Lett. 4 (1):29–32.

    CAS  Google Scholar 

  • Andersch, W., Bahl, H., and Gottschalk, G., 1983, Level of enzymes involved in acetate, butyrate, acetone and butanol formation by Clostridium acetobutylicum, Eur. J. Appl. Microbiol. Biotechnol. 18:327–332.

    CAS  Google Scholar 

  • Antranikian, G., Friese, C., Quentmeier, A., Hippe, H., and Gottschalk, G., 1984, Distribution of the ability for citrate utilization amongst Clostridia, Arch. Microbiol. 138:179–182.

    CAS  Google Scholar 

  • Baer, S. H., Blaschek, H. P., and Smith, T. L., 1987, Effect of butanol challenge and temperature of lipid composition and membrane fluidity of butanol-tolerant Clostridium acetobutylicum, Appl. Env. Microbiol. 53:2854–2861.

    CAS  Google Scholar 

  • Bahl, H., and Gottschalk, G., 1985, Chapaumeters affecting solvent production by Clostridium acetobutylicum in continuous culture, Biotechnol. Bioeng. 514:217–223.

    Google Scholar 

  • Bahl, H., Andersch, W., and Gottschalk, G., 1982, Continuous production of acetone and butanol by Clostridium acetobutylicum in a two-stage phosphate limited chemostat, Eur. J. Appl. Microbiol. Biotechnol. 15:201–205.

    CAS  Google Scholar 

  • Bahl, H., Gottwald, M., Kuhn, A., Rale, V., Andersch, W., and Gottschalk, G., 1986, Nutritional factors affecting the ratio of solvents produced by Clostridium acetobutylicum, Appl. Env. Microbiol. 52(1): 169–172.

    CAS  Google Scholar 

  • Ballongue, J., Amine, J., Masion, E., Petitdemange, H., and Gay, R., 1985, Induction of acetoacetate decarboxylase in Clostridium acetobutylicum, FEMS Microbiol. Lett. 29:273–277.

    CAS  Google Scholar 

  • Ballongue, J., Amine, J., Petitemange, H., and Gay, R., 1986, Regulation of acetate kinase and butyrate kinase by acids in Clostridium acetobutylicum. FEMS Microbiol. Lett. 35:295–301.

    CAS  Google Scholar 

  • Ballongue, J., Maison, E., Amine, J., Petitdemange, H., and Gay, R., 1987, Inhibitor effects of products of metabolism on growth of Clostridium acetobutylicum, Appl. Microbiol. Biotechnol. 26:568–573.

    CAS  Google Scholar 

  • Bayer, E. A., Setter, E., and Lamed, R., 1985, Organization and distribution of the cellulosome in Clostridium thermocellum, J. Bacteriol. 163(2):552–559.

    PubMed  CAS  Google Scholar 

  • Beesch, S. C., 1952, Acetone-butanol fermentation of sugars, Eng. Proc. Dev. 44:1677–1682.

    CAS  Google Scholar 

  • Beesch, S. C., 1953, Acetone-butanol fermentation of starches, Appl. Microbiol. 1:85–95.

    PubMed  CAS  Google Scholar 

  • Bowles, L. K., and Ellefson, W. L., 1985, Effects of butanol on Clostridium acetobutylicum, Appl. Env. Microbiol. 50(5): 1165–1170.

    CAS  Google Scholar 

  • Brener, D., and Johnson, B. F., 1984, Relationship between substrate and concentration and fermentation product ratios in Clostridium thermocellum cultures, Appl. Env. Microbiol. 47 (5): 1126–1129.

    CAS  Google Scholar 

  • Chen, J. S., and Hiu, S. F., 1986, Acetone-butanol-isopropanol production by Clostridium beijerinckii (synonym, Clostridium butylicum), Biotechnol. Lett. 8 (5):371–376.

    CAS  Google Scholar 

  • Clarke, K. G., 1987, A reassessment of the production of acetone and butanol by Clostridium acetobutylicum in continuous culture, Ph.D. thesis, University of Cape Town, South Africa, pp. 1–195.

    Google Scholar 

  • Clarke, K. G., and Hansford, G. S., 1986, Production of acetone and butanol by Clostridium acetobutylicum in a product limited chemostat, Chem. Eng. Commun. 45:75–81.

    CAS  Google Scholar 

  • Clarke, K. G., Hansford, G. S., and Jones, D. T., 1988, The nature and significance of oscillatory behavior during solvent production by Clostridium acetobutylicum in Continuous culture, Biotechnol. Bioeng. 32:538–544.

    PubMed  CAS  Google Scholar 

  • Conway, T., Sewell, G. W., Osman, Y. A., and Ingram, L. O., 1987, Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis, J. Bacteriol. 169:2591–2597.

    PubMed  CAS  Google Scholar 

  • Corry, J. E. L., 1978, Possible sources of ethanol ante- and post-mortem: Its relationship to the biochemistry and microbiology of decomposition, J. Appl. Bacteriol. 44:1–56.

    PubMed  CAS  Google Scholar 

  • Cummins, C. S., and Johnson, J. L., 1971, Taxonomy of the Clostridia: wall composition and DNA homologies in Clostridium butyricum and other butyric acid-producing Clostridia, J. Gen. Microbiol. 67:33–46.

    Google Scholar 

  • Datta, R., and Zeikus, J. G., 1985, Modulation of acetone-butanol-ethanol fermentation by carbon monoxide and organic acids, Appl. Environ. Microbiol. 49(3):522–529.

    PubMed  CAS  Google Scholar 

  • Davies, R., 1943, Studies on the acetone-butanol fermentation. 4. Acetoacetic acid decarboxylase of Cl. acetobutylcium (BY), Biochem. J. 37:230–238.

    PubMed  CAS  Google Scholar 

  • Doelle, H. W. (ed.), 1975, Bacterial Metabolism, 2nd ed., Academic Press, New York.

    Google Scholar 

  • Doremus, M. G., Linden, J. C., and Moreira, A. R., 1985, Agitation and pressure effects on acetone-butanol fermentation, Biotechnol. Bioeng. 27:852–860.

    PubMed  CAS  Google Scholar 

  • Dürre, P., Kuhn, A., and Gottschalk, G., 1986, Treatment with allyl alcohol selects specifically for mutants of Clostridium acetobutylicum defective in butanol synthesis, FEMS Microbiol. Lett. 36:77–81.

    Google Scholar 

  • Dürre, P., Kuhn, A., Gottwald, M., and Gottschalk, G., 1987, Enzymatic investigations on butanol dehydrogenase and butyraldehyde dehydrogenase in extracts of Clostridium acetobutylicum, Appl. Microbiol. Biotechnol. 26: 268–272.

    Google Scholar 

  • Dyr, J., Protiva, J., and Praus, R., 1958, Formation of neutral solvents in continuous fermentation by means of Clostridium acetobutylicum, in: Continuous Cultivation of Microorganisms (I. Malek, ed.), Czechoslovakian Academy of Sciences, Prague, pp. 210–226.

    Google Scholar 

  • Ennis, B. M., and Maddox, I. S., 1985, Use of Clostridium acetobutylicum P262 for production of solvents from whey permeate, Biotechnol. Lett. 7(8):601–606.

    CAS  Google Scholar 

  • Ennis, B. M., and Maddox, I. S., 1987, The effect of pH and lactose concentration on solvent production from whey permeate using Clostridium acetobutylicum, Biotechnol. Bioeng. 29: 329–334.

    PubMed  CAS  Google Scholar 

  • Ennis, B. M., Gutierrez, N. A., and Maddox, I. S., 1986, The acetone-butanol-ethanol fermentation: A current assessment, Process Biochem. October: 131–147.

    Google Scholar 

  • Ennis, B. M., Qureshi, N., and Maddox, I. S., 1987, In-line toxic product removal during solvent production by continuous fermentation using immobilized Clostridium acetobutylicum, Enzyme Microb. Technol. 9:672–675.

    CAS  Google Scholar 

  • Fick, M., Pierrot, P., and Engasser, J. M., 1985, Optimal conditions for long-term stability of acetone-butanol production by continuous cultures of Clostridium acetobutylicum, Biotechnol. Lett. 7 (7):503–508.

    CAS  Google Scholar 

  • Finn, R. K., and Nowrey, J. E., 1958, A note on the stability of Clostridia when held in continuous culture, Appl. Microbiol. 7:29–32.

    Google Scholar 

  • Fond, O., Petitdemange, E., Petitdemange, H., and Engasser, J. M., 1983, Cellulose fermentation by a coculture of a mesophilic cellulolytic Clostridium and Clostridium acetobutylicum, Biotechnol. Bioeng. Symp. 13:217–224.

    CAS  Google Scholar 

  • Fond, O., Matta-Ammouri, G., Petitdemange, H., and Engasser, J. M., 1985, The role of acids on the production of acetone and butanol by Clostridium acetobutylicum. Appl. Microbiol. Biotechnol. 22(3): 195–200.

    CAS  Google Scholar 

  • Forberg, C., Enfors, S. O., and Haggstrom, L., 1983, Control of immobilized, non-growing cells for continuous production of metabolites, Eur. J. Appl. Microbiol. Biotechnol. 17:143–147.

    Google Scholar 

  • Freier, D., and Gottschalk, G., 1987, L(+)-lactate dehydrogenase of Clostridium acetobutylicum is activated by fructose-1,6-bisphosphate, FEMS Microbiol. Lett. 43:229–233.

    CAS  Google Scholar 

  • Garcia, A., Iannotti, E. L., and Fischer, J. L., 1986, Butanol fermentation liquor production and seChapaution by reverse osmosis, Biotechnol. Bioeng. 28:785–791.

    PubMed  CAS  Google Scholar 

  • George, H. A., and Chen, J. S., 1983, Acidic conditions are not obligatory for onset of butanol formation by Clostridium beijerinckii (Synonym, C. butylicum), Appl. Environ. Microbiol. 46 (2):321–327.

    PubMed  CAS  Google Scholar 

  • George, H. A., Johnson, J. L., Moore, W. E. C., Holdeman, L. V., and Chen, J. S., 1983, Acetone, isopropanol, and butanol production by Clostridium beijerinckii (syn. Clostridium butylicum) and Clostridium aurantibutyricum, Appl. Environ. Microbiol. 45(3): 1160–1163.

    PubMed  CAS  Google Scholar 

  • Germain, P., Toukourou, F., and Donaduzzi, L., 1986, Ethanol production by anaerobic thermophilic bacteria: Regulation of lactate dehydrogenase activity in Clostridium thermohydrosulfuricum, Appl. Microbiol. Biotechnol. 24:300–305.

    CAS  Google Scholar 

  • Gibbs, D. F., 1983, The rise and fall (. . . and rise?) of acetone/butanol fermentations, Trends Biotechnol. 1:12–15.

    CAS  Google Scholar 

  • Gottschal, J. C., and Morris, J. G., 1981, The induction of acetone and butanol production in cultures of Clostridium acetobutylicum by elevated concentrations of acetate and butyrate, FEMS Microbiol. Lett. 12:385–389.

    CAS  Google Scholar 

  • Gottschal, J. C., and Morris, J. G., 1982, Continuous production of acetone and butanol by Clostridium acetobutylicum growing in turbidostat culture, Biotechnol. Lett. 4(8):477–482.

    CAS  Google Scholar 

  • Gottschalk, G., 1979, Butyrate and butanol-acetone fermentation, in: Bacteriol Metabolism, (M. P. Starr, ed.), Springer-Verlag, Berlin, pp. 182–215.

    Google Scholar 

  • Gottschalk, G., Andreesen, J. R., and Hippe, H., 1981, The genus Clostridium (nonmedical aspects), in: The Prokaryotes (M. P. Starr, H. Stolp, H. G. Truper, A. Balows, and H. G. Schlegel, eds.), Springer-Verlag, Berlin, pp. 1767–1803.

    Google Scholar 

  • Gottwald, M., Hippe, H., and Gottschalk, G., 1984, Formation of n-butanol from D-glucose by strains of ‘Clostridium tetanomorphum’ group, Appl. Env. Microbiol. 48 (3):573–576.

    CAS  Google Scholar 

  • Gottwald, M., and Gottschalk, G., 1985, The internal pH of Clostridium acetobutylicum and its effect on the shift from acid to solvent formation, Arch. Microbiol. 143:42–46.

    CAS  Google Scholar 

  • Griffith, W. L., Compere, A. L., and Googin, J. M., 1983, Novel neutral solvents fermentations, Dev. Ind. Microbiol. 24:347–352.

    CAS  Google Scholar 

  • Groot, W. J., and Luyben, K. C. A. M., 1987, Continuous production of butanol from a glucose/xylose mixture with an immobilized cell system coupled to pervaporation, Biotechnol. Lett. 9:867–870.

    CAS  Google Scholar 

  • Groot, W. J., Schoutens, G. H., Van Beelen, P. N., Van den Oever, C. E., and Kossen, N. W. F., 1984a, Increase of substrate conversion by pervaporation in the continuous butanol fermentation, Biotechnol. Lett. 6(12):789–792.

    CAS  Google Scholar 

  • Groot, W. J., van den Oever, C. E., and Kossen, N. W. F., 1984b, Pervaporation for simultaneous product recovery in the butanol/isopropanol batch fermentation, Biotechnol. Lett. 6(11):709–714.

    CAS  Google Scholar 

  • Haggström, L., and Enfors, S. O., 1982, Continuous production of butanol with immobilized cells of Clostridium acetobutylicum, Appl. Biochem. Biotechnol. 7:35–37.

    Google Scholar 

  • Haggström, L., and Molin, N., 1980, Calcium alginate immobilized cells of Clostridium acetobutylicum for solvent production, Biotechnol. Lett. 2:241–246.

    Google Scholar 

  • Hartmanis, M. G. N., 1987, Butyrate Kinase from Clostridium acetobutylicum, J. Biol. Chem. 262: 617–621.

    PubMed  CAS  Google Scholar 

  • Hartmanis, M. G. N., and Gatenbeck, S., 1984, Intermediary metabolism in Clostridium acetobutylicum: Levels of enzymes involved in the formation of acetate and butyrate, Appl. Env. Microbiol. 47(6): 1277–1283.

    CAS  Google Scholar 

  • Hartmanis, M. G. N., Klason, T., and Gatenbeck, S., 1984, Uptake and activation of acetate and butyrate in Clostridium acetobutylicum, Appl. Microbiol. Biotechnol. 20(1):66–71.

    CAS  Google Scholar 

  • Hartmanis, M. G. N., Ahlman, H., and Gatenbeck, S., 1986, Stability of solvent formation in Clostridium acetobutylicum during repeated subculturing, Appl. Microbiol. Biotechnol. 23: 369–371.

    CAS  Google Scholar 

  • Hastings, J. H. J., 1978, Acetone-butyl alcohol fermentation, in: Economic Microbiology, Primary Products of Metabolism, Vol. 2 (A. H. Rose, ed.), Academic Press, New York, pp. 31–45.

    Google Scholar 

  • Hermann, M., Fayolle, F., Marchal, R., Podvin, L., Sebald, M., and Vandecasteele, J. P., 1985, Isolation and characterization of butanol-resistant mutants of Clostridium acetobutylicum, Appl. Env. Microbiol. 50(5): 1238–1243.

    CAS  Google Scholar 

  • Herrero, A. A., 1983, End-product inhibition in anaerobic fermentations, Trends Biotechnol. 1 (2):49–53.

    CAS  Google Scholar 

  • Herrero, A. A., and Gomez, R. F., 1980, Development by ethanol tolerance in Clostridium thermocellum: Effect of growth temperature, Appl. Env. Microbiol. 40:571–577.

    CAS  Google Scholar 

  • Herrero, A. A., Gomez, R. F., and Roberts, M. F., 1982, Ethanol-induced changes in the membrane lipid composition of Clostridium thermocellum, Biochim. Biophys. Acta 693:195–204.

    PubMed  CAS  Google Scholar 

  • Herrero, A. A., Gomez, R. F., and Roberts, M. F., 1985, 31P-NMR studies of Clostridium thermocellum: Mechanisms of endproduct inhibition by ethanol, J. Biol. Chem. 260:7442–7451.

    PubMed  CAS  Google Scholar 

  • Hiu, S. F., Zhu, C-X., Yan, R-T., and Chen, J-S., 1987, Butanol-ethanol dehydrogenase and butanol-ethanol-isopropanol dehydrogenase: Different alcohol dehydrogenases in two strains of Clostridium beijerinckii (Clostridium butylicum), Appl. Env. Microbiol. 53:697–703.

    CAS  Google Scholar 

  • Holdeman, L. V., Cato, E. P., Moore, W. E. C. (eds.), 1977, Anaerobe Laboratory Manual, 4th Ed., Virginia Polytechnic Institute, Blacksburg, Virginia.

    Google Scholar 

  • Holt, R. A., Stephens, G. M., and Morris, J. G., 1984, Production of solvents by Clostridium acetobutylicum cultures maintained at neutral pH, Appl. Env. Microbiol. 48(6): 1166–1170.

    CAS  Google Scholar 

  • Hon-nami, K., Coughlan, M. P., Hon-nami, H., and Ljungdahl, L. G., 1986, Sechapaution and characterization of the complexes constituting the cellulolytic enzyme system of Clostridium thermocellum, Arch. Microbiol. 145:13–19.

    CAS  Google Scholar 

  • Hospodka, J., 1966, Industrial application of continuous fermentation, in: Theoretical and Methodological Bases of Continuous Culture of Microorganisms (I. Malek and Z. Fencl, eds.), Academic Press, New York, pp. 611–613.

    Google Scholar 

  • Hsu, E. J., and Ordal, Z. J., 1970, ComChapautive metabolism of vegetative and sporulating cultures of Clostridium thermosaccharolyticum, J. Bacteriol. 102(2):369–376.

    PubMed  CAS  Google Scholar 

  • Huang, L., Forsberg, C. W., and Gibbins, L. N., 1986, Influence of external pH and fermentation products on Clostridium acetobutylicum intracellular pH and cellular distribution of fermentation products, Appl. Env. Microbiol. 51 (6): 1230–1234.

    CAS  Google Scholar 

  • Hugo, H. V., Schoberth, S., madan, V. K., and Gottschalk, G., 1972, Coenzyme specificity of dehydrogenases and fermentation of pyruvate by Clostridia, Arch. Mikrobiol. 87:189–202.

    Google Scholar 

  • Hungate, R. E., 1944, Studies on cellulose fermentation. I. The culture and physiology of an anaerobic cellulose-digesting bacterium, J. Bacteriol. 48:499–513.

    PubMed  CAS  Google Scholar 

  • Hutkins, R. W., and Kashket, E. R., 1986, Phosphotransferase activity in A. acetobutylicum from acidogenic and solventogenic phase of growth, Appl. Env. Microbiol. 51:1121–1123.

    CAS  Google Scholar 

  • Hyun, H. H., and Zeikus, J. G., 1985, Simultaneous and enhanced production of thermostable amylases and ethanol from starch by cocultures of Clostridium thermosulfurogenes and Clostridium thermohydrosulfuricum, Appl. Env. Microbiol. 49 (5): 1174–1181.

    CAS  Google Scholar 

  • Ingram, L. O., 1986, Microbial tolerance to alcohols: Role of the cell membrane, TIBTECH. Feb:40–44.

    Google Scholar 

  • Ingram, L. O., and Buttke, T. M., 1984, Effects of alcohols on microorganisms, Adv. Microb. Physiol. 25:256–300.

    Google Scholar 

  • Janati-Idrissi, R., Junelles, A. M., El Kanouni, A., Petitdemange, H., and Gay, R., 1987, Selection de mutants de Clostridium acetobutylicum defectifs dans la production d’acetone, Ann. Inst. Pasteurl Microbiol. 138:313–323.

    CAS  Google Scholar 

  • Jobses, I. M. L., and Roels, J. A., 1983, Experience with solvent production by Clostridium beijerinckii in continuous culture, Biotechnol. Bioeng. 25:1187–1194.

    PubMed  CAS  Google Scholar 

  • Jones, D. T., and Woods, D. R., 1986, The acetone butanol fermentation revisited, Microbiol. Rev. 50:484–524.

    PubMed  CAS  Google Scholar 

  • Jones, D. T., van der Westhuizen, A., Long, S., Allcock, E. R., Reid, S. J., and Woods, D. R., 1982, Solvent production and morphological changes in Clostridium acetobutylicum, Appl. Environ. Microbiol. 43 (6): 1434–1439.

    PubMed  CAS  Google Scholar 

  • Jungermann, K., Thauer, R. K., Leimenstoll, G., and Decker, K., 1973, Function of reduced pyridine nucleotide-ferredoxin oxidoreductases in saccharolytic Clostridia, Biochim. Bi-ophys. Acta. 305:268–280.

    CAS  Google Scholar 

  • Kell, D. B., Peck, M. W., Rodger, G., and Morris, J. G., 1981, On the permeability to weak acids and bases of the cytoplasmic membrane of Clostridium pasteurianum, Biochem. Biophys. Res. Commun. 99:81–88.

    PubMed  CAS  Google Scholar 

  • Kelly, W. J., Asmundson, R. V., and Hopcroft, D. H., 1987, Isolation and characterization of a strictly anaerobic, cellulolytic spore former: Clostridium chartatabidium sp. nov., Arch. Microbiol. 147:169–173.

    PubMed  CAS  Google Scholar 

  • Kim, B. H., and Zeikus, J. G., 1985, Importance of hydrogen metabolism in regulation of solventogenesis by Clostridium acetobutylicum, Dev. Ind. Microbiol. 26:1–14.

    Google Scholar 

  • Kim, B. H., Bellows, P., Datta, R., and Zeikus, J. G., 1984, Control of carbon and electron flow in Clostridium acetobutylicum fermentations: Utilization of carbon monoxide to inhibit hydrogen production and to enhance butanol yields, Appl. Env. Microbiol. 48 (4): 764–770.

    CAS  Google Scholar 

  • Krouwel, P. G., Van der Laan, W. F. M., and Kossen, N. W. F., 1980, Continuous production of n-butanol and isopropanol by immobilized, growing Clostridium butylicum cells, Biotechnol. Lett. 2:253–258.

    CAS  Google Scholar 

  • Krouwel, P. G., Groot, W. J., Kossen, N. W. F., and van der Laan, W. F. M., 1983, Continuous isopropanol-butanol-ethanol fermentation by immobilized Clostridium beijerinckii cells in a packed bed fermenter, Enzyme Microb. Technol. 5:46–55.

    CAS  Google Scholar 

  • Kutzenok, A., and Aschner, M., 1952, Degenerative processes in a strain of Clostridium butylicum, J. Bacteriol. 64:829–836.

    PubMed  CAS  Google Scholar 

  • Lamed, R. J., and Zeikus, J. G., 1980, Novel NADP-linked alcohol aldehyde/ketone oxidoreductase in thermophilic, ethanolgenic bacteria, Biochem. J. 195:183–190.

    Google Scholar 

  • Lamed, R., Setter, E., and Bayer, E. A., 1983, Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum, J. Bacteriol. 156 (2):828–836.

    PubMed  CAS  Google Scholar 

  • Landuyt, S. L., Hsu, E. J., and Lu, M., 1983, Transition from acid fermentation to solvent fermentation in a continuous dilution culture of Clostridium thermosaccharolyticum, Ann. N. Y. Acad. Sci. 413:473–478.

    PubMed  CAS  Google Scholar 

  • Largier, S. T., Long, S., Santangelo, J. D., Jones, D. T., and Woods, D. R., 1985, Immobilized Clostridium acetobutylicum P262 mutants for solvent production, Appl. Environ. Microbiol. 50 (2):477–481.

    PubMed  CAS  Google Scholar 

  • Larrayoz, M. A., and Puigjaner, L., 1987, Study of butanol extraction through pervaporation in acetobutylic fermentation, Biotechnol. Bioeng. 30:692–696.

    PubMed  CAS  Google Scholar 

  • Lee, S. F., and Forsfrerg, C. W., 1987, Isolation and some properties of a B-D-xylosidase from Clostridium acetobutylicum ATCC 824, Appl. Env. Microbiol. 53:651–654.

    CAS  Google Scholar 

  • Lee, S. F., Forsberg, C. W., and Gibbins, L. N., 1985, Cellulolytic activity of Clostridium acetobutylicum, Appl. Env. Microbiol. 50(2):220–228.

    CAS  Google Scholar 

  • Lemmel, S. A., Datta, R., and Frankiewicz, J. R., 1986, Fermentation of xylan by Clostridium acetobutylicum, Enzyme Microb. Technol. 8:217–221.

    CAS  Google Scholar 

  • Lenz, T. G., and Moreira, A. R., 1980, Economic evaluation of the acetone-butanol fermentation, Ind. Eng. Chem. Prod. Res. Dev. 19:478–483.

    CAS  Google Scholar 

  • LeChapaffil, C., Fayolle, F., Hermann, M., and Vandecasteele, J-P., 1987, Changes in membrane lipid composition of Clostridium acetobutylicum during acetone-butanol fermentation: Effects of solvents, growth temperature and pH, J. Gen. Microbiol. 133:103–110.

    Google Scholar 

  • Lin, Y., and Blaschek, H. P., 1984, Butanol production by a butanol-tolerant strain of Clostridium acetobutylicum in extruded corn broth, Appl. Env. Microbiol. 45 (3):966–973.

    Google Scholar 

  • Long, S., Jones, D. T., and Woods, D. R., 1983, Sporulation of Clostridium acetobutylicum P262 in a defined medium, Appl. Env. Microbiol. 45(4): 1389–1393.

    CAS  Google Scholar 

  • Long, S., Jones, D. T., and Woods, D. R., 1984a, The relationship between sporulation and solvent production in Clostridium acetobutylicum P262, Biotechnol. Lett. 6 (8):529–534.

    CAS  Google Scholar 

  • Long, S., Jones, D. T., and Woods, D. R., 1984b, Initiation of solvent production, clostridial stage and endospore formation in Clostridium acetobutylicum P262, Appl. Microbiol. Biotechnol. 20 (4):256–261.

    CAS  Google Scholar 

  • Lovitt, R. W., Longin, R., and Zeikus, J. G., 1984, Ethanol production by thermophilic bacteria: Physiological comparison of solvent effects on parent and alcohol-tolerant strains of Clostridium thermohydrosulfuncum, Appl. Environ. Microbiol. 48 (1): 171–177.

    PubMed  CAS  Google Scholar 

  • Maddox, I. S., 1983, Use of silicalite for the adsorption of n-butanol from fermentation liquors, Biotechnol. Lett. 5:89–94.

    Google Scholar 

  • Maddox, I. S., and Murray, A. E., 1983, Production of n-butanol by fermentation of wood hydrolysate, Biotechnol. Lett. 5 (3): 175–178.

    CAS  Google Scholar 

  • Marchal, R., Rebeller, M., and Vandecasteele, J. P., 1984, Direct bioconversion of alkali-pretreated straw using simultaneous enzymatic hydrolysis and acetone-butanol fermentation, Biotechnol. Lett. 6 (8):523–528.

    CAS  Google Scholar 

  • Marchal, R., Blanchet, D., and Vandecasteele, J. P., 1985, Industrial optimization of acetone-butanol fermentation: A study of the utilization of Jerusalem artichokes, Appl. Microbiol. Biotechnol. 23:92–98.

    CAS  Google Scholar 

  • Marchal, R., Ropars, M., and Vandecasteele, J. P., 1986, Conversion into acetone and butanol of lignocellulosic substrates pretreated by steam explosion, Biotechnol. Lett. 8 (5):365–370.

    CAS  Google Scholar 

  • Marlatt, J. A., and Datta, R., 1986, Acetone-butanol fermentation process development and economic evaluation, Biotechnol. Prog. 2:23–28.

    PubMed  CAS  Google Scholar 

  • Masion, E., Amine, J., and Marczak, R., 1987, Influence of amino acid supplements on the metabolism of Clostridium acetobutylicum, FEMS Microbiol. Lett. 43:269–274.

    CAS  Google Scholar 

  • Matta-El-Amouri, G., Janati-Idrissi, R., Assobhei, O., Petitdemange, H., and Gay, R., 1985, Mechanism of the acetone formation by Clostridium acetobutylicum, FEMS Microbiol Lett. 30:11–16.

    CAS  Google Scholar 

  • Matta-El-Ammouri, G., Janati-Idrissi, R., Junelles, A.-M., Petitdemange, H., and Gay, R., 1987, Effects of butyric and acetic acids on acetone-butanol formation by Clostridium acetobutylicum, Biochimie 69:109–115.

    PubMed  CAS  Google Scholar 

  • Mattiasson, B., 1983, Applications of aqueous two-phase systems in biotechnology, Trends Biotechnol. 1 (1): 16–20.

    CAS  Google Scholar 

  • Mattiasson, B., Suominen, M., Andersson, E., Haggstrom, L., Albertsson, P. A., and Hahn-Hagerdal, B., 1982, Solvent production by Clostridium acetobutylicum in aqueous two-phase system, Enzyme Eng. 6:153–155.

    CAS  Google Scholar 

  • McCoy, E., and Fred, B., 1941, The stability of a culture for industrial fermentation, J. Bacterial. 41:90–91.

    Google Scholar 

  • McCutchan, W. N., and Hickey, R. J., 1954, The butanol-acetone fermentations, Ind. Ferment. 1:347–388.

    Google Scholar 

  • McNeil, B., and Kristiansen, B., 1986, The acetone butanol fermentation, in: Advances in Applied Microbiology, Vol. 31 (A. Laskin, ed.), Academic Press, New York, pp. 61–92.

    Google Scholar 

  • Meyer, C. L., Roos, J. W., and Papoutsakis, E. T., 1986, Carbon monoxide gasing leads to alcohol production and butyrate uptake without acetone formation in continuous cultures of Clostridium acetobutylicum. Appl. Microbiol. Biotechnol. 24:159–167.

    CAS  Google Scholar 

  • Monot, F., and Engasser, J. M., 1983a, Production of acetone and butanol by batch and continuous culture of Clostridium acetobutylicum under nitrogen limitation, Biotechnol. Lett. 5 (4):213–218.

    CAS  Google Scholar 

  • Monot, F., and Engasser, J. M., 1983b, Continuous production of acetone butanol on an optimized synthetic medium, Eur. J. Appl. Microbiol. Biotechnol. 18:246–248.

    CAS  Google Scholar 

  • Monot, F., Martin, J.R., Petitdemange, H., and Gay, R., 1982, Acetone and butanol production by Clostridium acetobutylicum in a synthetic medium, Appl. Env. Microbiol. 44 (6): 1318–1324.

    CAS  Google Scholar 

  • Monot, F., Engasser, J. M., and Petitdemange, H., 1983, Regulation of acetone butanol production in batch and continuous cultures of Clostridium acetobutylicum, Biotechnol. Bioeng. Symp. 13:207–216.

    CAS  Google Scholar 

  • Monot, F., Engasser, J. M., and Petitdemange, H., 1984, Influence of pH and undissociated butyric acid on the production of acetone and butanol in batch cultures of Clostridium acetobutylicum, Appl. Microbiol. Biotechnol. 19 (6):422–426.

    CAS  Google Scholar 

  • Moreira, A. R., 1983, Acetone-butanol fermentation, in: Organic Chemicals From Biomass (D. L. Wise, ed.), Benjamin/Cummings, Menlo Park, CA, pp. 385–406.

    Google Scholar 

  • Moreira, A. R., Ulmer, D. C., and Linden, J. C., 1981, Butanol toxicity in the butylic fermentation, Biotechnol. Bioeng. Symp. 11:567–579.

    CAS  Google Scholar 

  • Moreira, A. R., Dale, B. E., and Doremus, M. G., 1982, Utilization of the fermentor off-gases from an acetone-butanol fermentation, Biotechnol. Bioeng. Symp. 12:263–277.

    CAS  Google Scholar 

  • Murray, W. D., and Khan, A. W., 1983a, Ethanol production by a newly isolated anaerobe, Clostridium saccharolyticum: Effects of culture medium and growth conditions, Can. J. Microbiol. 29:342–347.

    CAS  Google Scholar 

  • Murray, W. D., and Khan, A. W., 1983b, Growth requirements of Clostridium saccharolyticum, an ethanologenic anaerobe, Can. J. Microbiol. 29:348–353.

    CAS  Google Scholar 

  • Murray, W. D., Wemyss, K. B., and Khan, A. W., 1983, Increased ethanol production and tolerance by a pyruvate-negative mutant of Clostridium saccharolyticum, Eur. J. Appl. Microbiol. Biotechnol. 18:71–74.

    CAS  Google Scholar 

  • Ng, T. K., and Zeikus, J. G., 1982, Differential metabolism of cellobiose and glucose by Clostridium thermocellum and Clostridium thermohydrosulfuricum, J. Bacteriol. 150 (3): 1391–1399.

    PubMed  CAS  Google Scholar 

  • Ng, T. K., Weimer, P. J., and Zeikus, J. G., 1977, Cellulolytic and physiological properties of Clostridium thermocellum, Arch. Microbiol. 114:1–7.

    PubMed  CAS  Google Scholar 

  • Ng, T. K., Ben-Bassat, A., and Zeikus, J. G., 1981, Ethanol production by thermophilic bacteria fermentation of cellulose substrates by co-cultures of Clostridium thermocellum and C. thermohydrosulfuricum, Appl. Env. Microbiol. 42:231–240.

    CAS  Google Scholar 

  • Ounine, K., Petitdemange, H., Raval, G., and Gay, R., 1985, Regulation and butanol inhibition of D-xylose and D-glucose uptake in Clostridium acetobutylicum, Appl. Env. Microbiol. 49 (4):874–878.

    CAS  Google Scholar 

  • Palosaari, N. R., and Rogers, P., 1988, Purification and properties of the inducible coenzyme A-linked butyraldehyde dehydrogenase from Clostridium acetobutylicum, J. Bacteriol. 170: 2971–2976.

    PubMed  CAS  Google Scholar 

  • Parkkinen, E., 1986, Conversion of starch into ethanol by Clostridium thermohydrosulfuricum, Appl. Microbiol. Biotechnol. 25:213–219.

    CAS  Google Scholar 

  • Patni, N. J., and Alexander, J. K., 1971, Catabolism of fructose and mannitol in Clostridium thermocellum: Presence of phosphoenolpyruvate: Fructose phosphotransferase, fructose 1-phosphate kinase, phosphoenolpyruvate: Mannitol phosphotransferase, and mannitol 1-phosphate dehydrogenase in cell extracts, J. Bacteriol. 105 (1):226–231.

    PubMed  CAS  Google Scholar 

  • Petitdemange, E., Caillet, F., Giallo, J., and Gaudin, C., 1984, Clostridium cellulolyticum sp. nov., a cellulolytic, mesophilic species from decayed grass, Int. J. Syst. Bacteriol. 34 (2): 155–159.

    Google Scholar 

  • Pierrot, P., Fick, M., and Engasser, J. M., 1986, Continuous acetone-butanol fermentation with high productivity by cell ultrafiltration and recycling, Biotechnol. Lett. 8 (4):253–256.

    CAS  Google Scholar 

  • Prescott, S. G., and Dunn, C. G., 1959, The acetone-butanol fermentation, in: Industrial Microbiology, McGraw-Hill, New York, pp. 180–214.

    Google Scholar 

  • Qureshi, N., and Maddox, I. S., 1987, Continuous solvent production from whey permeate using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar, Enzyme Microb. Technol. 9:668–671.

    CAS  Google Scholar 

  • Rao, G., and Mutharasan, R., 1987, Altered electron flow in continuous cultures of Clostridium acetobutylicum induced by viologen dyes, Appl. Env. Microbiol. 53 (6): 1232–1235.

    CAS  Google Scholar 

  • Reardon, K. F., Scheper, T.-H., and Bailey, J. E., 1987, Metabolic pathway rates and culture fluorescence in batch fermentations of Clostridium acetobutylicum, Biotechnol. Prog. 3:153–167.

    CAS  Google Scholar 

  • Reysenbach, A. L., Ravenscroft, N., Long, S., Jones, D. T., and Woods, D. R., 1986, Characterization, biosynthesis, and regulation of granulose in Clostridium acetobutylicum, Appl. Env. Microbiol. 52 (1): 185–190.

    CAS  Google Scholar 

  • Roffler, S. R., Blanch, H. W., and Wilke, C. R., 1987, Extractive fermentation of acetone and butanol: Process design and economic evaluation, Biotechnol. Prog., 3:131–140.

    CAS  Google Scholar 

  • Roffler, S. R., Blanch, H. W., and Wilke, C. R., 1988, In situ extractive fermentation of acetone and butanol, Biotechnol. Bioeng. 31:135–143.

    PubMed  CAS  Google Scholar 

  • Rogers, P., 1984, Genetics and biochemistry of Clostridium relevant to development of fermentation processes, in: Advances in Applied Microbiology (A. I. Laskin, ed.), Academic Press, New York, pp. 1–89.

    Google Scholar 

  • Rogers, P., and Palosaari, N., 1987, Clostridium acetobutylicum mutants that produce butyraldehyde and altered quantities of solvents, Appl. Env. Microbiol. 53:2761–2766.

    CAS  Google Scholar 

  • Ross, D., 1961, The acetone-butanol fermentation, Prog. Ind. Microbiol. 3:73–85.

    Google Scholar 

  • Rothstein, D. M., 1986, Clostridium thermosaccharolyticum strain deficient in acetate production, J. Bacteriol. 165 (1):319–320.

    PubMed  CAS  Google Scholar 

  • Ryden, R., 1958, Development of anaerobic fermentation processes: Acetone-butanol, in: Biochemical Engineering (R. Steel, ed.), Heywood, London, pp. 125–148.

    Google Scholar 

  • Schink, B., and Zeikus, J. G., 1983, Clostridium thermosulfurogenes sp. nov., a new thermophile that produces elemental sulphur from thiosulphate, J. Gen. Microbiol. 129:1149–1158.

    CAS  Google Scholar 

  • Scholote, D., and Gottschalk, G., 1986, Effect of cell recycle on continuous butanol-acetone fermentation with Clostridium acetobutylicum under phosphate limitation, Appl. Microbiol. Biotechnol. 24 (1): 1–6.

    Google Scholar 

  • Schoutens, G. H., Nieuwenhuizen, M. C. H., and Kossen, N. W. F., 1984, Butanol from whey ultrafiltrate: Batch experiments with Clostridium beyerinckii LMD 27.6, Appl. Microbiol. Biotechnol. 19:203–206.

    CAS  Google Scholar 

  • Schoutens, G. H., Nieuwenhuizen, M. C. H., and Kossen, N. W. F., 1985, Continuous butanol production from whey permeate with immobilized Clostridium beyerinckii LMD 27.6, Appl. Microbiol. Biotechnol. 21:282–286.

    CAS  Google Scholar 

  • Simon, E., 1947, The formation of lactic acid by Clostridium acetobutylicum (Weizman), Arch. Biochem. 13:237–243.

    PubMed  CAS  Google Scholar 

  • Sioumis, A. A., 1987, Recovery of alcohols: A chemical approach utilizing lactones, TIBTECH 5:215–217.

    CAS  Google Scholar 

  • Spivey, M. J., 1978, The acetone/butanol/ethanol fermentation, Process Biochem. 13 (11):2–5.

    CAS  Google Scholar 

  • Srinivas, S. P., and Mutharasan, R., 1987, Culture fluorescence characteristics and its metabolic significance in batch cultures of Clostridium acetobutylicum, Biotechnol. Lett. 9:139–142.

    CAS  Google Scholar 

  • Stephens, G. M., Holt, R. A., Gottschal, J. C., and Morris, J. G., 1985, Studies on the stability of solvent production by Clostridium acetobutylicum in continuous culture, J. Appl. Bacteriol. 59: 597–605.

    Google Scholar 

  • Taya, M., Ishii, S., and Kobayashi, T., 1985, Monitoring and control for extractive fermentation of Clostridium acetobutylicum, J. Ferment. Technol. 63 (2): 181–187.

    CAS  Google Scholar 

  • Terracciano, J. S., and Kashket, E. R., 1986, Intracellular conditions required for initiation of solvent production by Clostridium acetobutylicum, Appl. Env. Microbiol. 52 (1):86–91.

    CAS  Google Scholar 

  • Terracciano, J. S., Schreurs, W.J. A., and Kasket, E. R., 1987, Membrane H+ conductance of Clostridium thermoaceticum and Clostridium acetobutylicum: Evidence for electrogenic Na+/H+ antiport in Clostridium thermoaceticum, Appl. Env. Microbiol. 53:782–786.

    CAS  Google Scholar 

  • Turunen, M., Parkkinen, E., Londesborough, J., and Korhola, M., 1987, Distinct forms of lactate dehydrogenase purified from ethanol- and lactate-producing cells of Clostridium thermohydrosulfuricum, J. Gen. Microbiol. 133:2865–2873.

    CAS  Google Scholar 

  • Van der Westhuizen, A., Jones, D. T., and Woods, D. R., 1982, Autolytic activity and butanol tolerance of Clostridium acetobutylicum, Appl. Env. Microbiol. 44 (6): 1277–1282.

    Google Scholar 

  • Volesky, B., and Szczesny, T., 1983, Bacterial conversion of pentose sugars to acetone and butanol, Adv. Biochem. Eng./Biotechnol. 27:101–117.

    CAS  Google Scholar 

  • Volesky, B., Mulchandani, A., and Williams, J., 1981, Biochemical production of industrial solvents (acetone-butanol-ethanol) from renewable resources, Ann. N.Y. Acad. Sci. 369: 205–218.

    CAS  Google Scholar 

  • Vollherbst-Schneck, K., Sands, J. A., and Montenecourt, B. S., 1984, Effect of butanol on lipid composition and fluiditty of Clostridium acetobutylicum ATCC 824, Appl. Env. Microbiol. 47: 193–194.

    CAS  Google Scholar 

  • Walther, R., Hippe, H., and Gottschalk, G., 1977, Citrate, a specific substrate for the isolation of Clostridium sphenoides, Appl. Env. Microbiol. 33:955–962.

    CAS  Google Scholar 

  • Walton, M. T., and Martin, J. L., 1979, Production of butanol-acetone by fermentation, in: Microbial Technology, Vol. 1 (H. J. Peppier and D. Perlman, eds.), Academic Press, New York, pp. 187–209.

    Google Scholar 

  • Wayman, M., and Parekh, R., 1987, Production of acetone-butanol by extractive fermentation using dibutylphthalate as extractant, J. Ferment. Technol. 65:295–300.

    CAS  Google Scholar 

  • Welsh, F. W., and Veliky, I. A., 1984, Production of acetone-butanol from acid whey, Biotechnol. Lett. 6 (1):61–64.

    CAS  Google Scholar 

  • Welsh, F. W., Williams, R. E., and Veliky, I. A., 1986, A note on the effect of nitrogen source on growth of and solvent production by Clostridium acetobutylicum, J. Appl. Bacteriol. 61: 413–419.

    CAS  Google Scholar 

  • Welsh, F. W., Williams, R. E., and Veliky, I. A., 1987, Organic and inorganic nitrogen source effects on the metabolism of Clostridium acetobutylicum, Appl. Microbiol. Biotechnol. 26:369–372.

    CAS  Google Scholar 

  • Wiegel, J., 1980, Formation of ethanol by bacteria. A pledge for the use of extreme thermophilic anaerobic bacteria in industrial ethanol fermentation process, Experientia 36: 1434–1446.

    CAS  Google Scholar 

  • Wiegel, J., Ljungdahl, L. G., and Rawson, J. R., 1979, Isolation from soil and properties of the extreme thermophile Clostridium thermohydrosulfuricum, J. Bacteriol. 139 (3):800–801.

    PubMed  CAS  Google Scholar 

  • Williamson, V. M., and Paquin, C. E., 1987, Homology of Saccharomyces cerevisiae ADH4 to an iron-activated alcohol dehydrogenase from Zymomonas mobilis, Mol. Gen. Genet. 209:374–381.

    PubMed  CAS  Google Scholar 

  • Yarovenko, V. L., 1964, Principles of the continuous alcohol and butanol-acetone fermentation processes, in: Continuous Cultivation of Microorganisms (I. Malek, ed.), Czechoslovakian Academy of Sciences, Prague, pp. 205–217.

    Google Scholar 

  • Yerushalmi, L., and Volesky, B., 1985, Importance of agitation in acetone-butanol fermentation, Biotechnol. Bioeng. 28:1297–1305.

    Google Scholar 

  • Yerushalmi, L., Volesky, B., and Szczesny, T., 1985, Effect of increased hydrogen partial pressure on the acetone-butanol fermentation by Clostridium acetobutylicum, Appl. Microbiol. Biotechnol. 22:103–107.

    CAS  Google Scholar 

  • Youngleson, J. S., Santangelo, J. D., Jones, D. T., and Woods, D. R., 1988, Cloning and expression of a Clostridium acetobutylicum alcohol dehydrogenase gene in Escherichia coli, Appl. Env. Microbiol 54:676–682.

    CAS  Google Scholar 

  • Yu, E. K. C., and Saddler, J. N., 1983, Enhanced acetone-butanol fermentation by Clostridium acetobutylicum grown on D-xylose in the presence of acetic or butyric acid, FEMS Microbiol. Lett. 18:103–107.

    CAS  Google Scholar 

  • Yu, E. K. C., Deschatelets, L., and Saddler, J. N., 1984, The bioconversion of wood hydrolyzates to butanol and butanediol, Biotechnol. Lett. 6 (5):327–332.

    CAS  Google Scholar 

  • Yu, E. K. C., Chan, M. K. H., and Saddler, J. N., 1985, Butanol production from cellulosic substrates by sequential coculture of Clostridium thermocellum and C. acetobutylicum, Biotechnol. Lett. 7 (7):509–514.

    CAS  Google Scholar 

  • Zappe, H., Jones, D. T., and Woods, D. R., 1986, Cloning and expression of Clostridium acetobutylicum endoglucanase, cellobiase and amino acid biosynthesis genes in Escherichia coli, J. Gen. Microbiol. 132:1367–1372.

    PubMed  CAS  Google Scholar 

  • Zappe, H., Jones, D. T., and Woods, D. R., 1987, Cloning and expression of a xylanase gene from Clostridium acetobutylicum P262 in Escherichia coli, J. Microbiol. Biotechnol. 27:57–63.

    CAS  Google Scholar 

  • Zeikus, J. G., 1979, Thermophilic bacteria: Ecology, physiology and technology, Enzyme Microb. Technol. 1:243–252.

    CAS  Google Scholar 

  • Zeikus, J. G., 1980, Chemical and fuel production by anaerobic bacteria, Annu. Rev. Microbiol. 34:423–464.

    PubMed  CAS  Google Scholar 

  • Zeikus, J. G., 1985, Biology of spore-forming anaerobes, in: Biology of Industrial Microorganisms (A. L. Demain and N. A. Solomon, eds.), Benjamin Cummings, Menlo Park, CA, pp. 79–114.

    Google Scholar 

  • Zeikus, J. G., Ben-Bassat, A., and Hegge, P.W., 1980, Microbiology of methanogenesis in thermal, volcanic environments, J. Bacteriol. 143 (1):432–440.

    PubMed  CAS  Google Scholar 

  • Zeikus, J. G., Ben-Bassat, A., Ng, T. K., and Lamed, R. J., 1981, Thermophilic ethanol fermentations, in: Trends in the Biology of Fermentations (A. Hollander, ed.), Plenum Press, New York, pp. 441–461.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Springer Science+Business Media New York

About this chapter

Cite this chapter

Jones, D.T., Woods, D.R. (1989). Solvent Production. In: Minton, N.P., Clarke, D.J. (eds) Clostridia. Biotechnology Handbooks, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9718-3_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-9718-3_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9720-6

  • Online ISBN: 978-1-4757-9718-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics