Methods for Measuring Chloride Transport across Nerve, Muscle, and Glial Cells

  • Francisco J. Alvarez-Leefmans
  • Fernando Giraldez
  • John M. Russell


Intracellular Cl together with HCO 3 is the most abundant free anion in living cells. Measuring intracellular chloride activity (a Cl i ) and studying the mechanisms involved in regulation of intracellular Cl is particularly important in excitable cells for four main reasons: (1) a Cl i is a quantity needed to determine E Cl, the Cl equilibrium potential. (2) Several transport mechanisms responsible for intracellular pH regulation are tightly coupled to Cl. (3) Cl is also involved in transport mechanisms implicated in cell volume regulation. (4) Knowledge of intracellular Cl homeostasis is crucial for understanding synaptic inhibition.


Dorsal Root Ganglion Neuron Liquid Membrane Selectivity Coefficient Membrane Electrode Filling Solution 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Francisco J. Alvarez-Leefmans
    • 1
    • 4
  • Fernando Giraldez
    • 2
  • John M. Russell
    • 3
  1. 1.Departamento de Farmacología y ToxicologíaCentro de Investigacíon y de Estudios Avanzados deo I. P. N.Mexico D. F.Mexico
  2. 2.Departamento de NeurobiologíaInstituto Mexicano de PsiquitríaMexico D. F.Mexico
  3. 3.Departamento de Bioquimica, Biología Molecular y FisiologíaUniversidad de ValladolidValladolidSpain
  4. 4.Department of Physiology and BiophysicsUniversity of Texas Medical BranchGalvestonUSA

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