Abstract
Intracellular Cl− together with HCO −3 is the most abundant free anion in living cells. Measuring intracellular chloride activity (a iCl ) and studying the mechanisms involved in regulation of intracellular Cl− is particularly important in excitable cells for four main reasons: (1) a iCl is a quantity needed to determine E Cl, the Cl− equilibrium potential. (2) Several transport mechanisms responsible for intracellular pH regulation are tightly coupled to Cl−. (3) Cl− is also involved in transport mechanisms implicated in cell volume regulation. (4) Knowledge of intracellular Cl− homeostasis is crucial for understanding synaptic inhibition.
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Alvarez-Leefmans, F.J., Giraldez, F., Russell, J.M. (1990). Methods for Measuring Chloride Transport across Nerve, Muscle, and Glial Cells. In: Alvarez-Leefmans, F.J., Russell, J.M. (eds) Chloride Channels and Carriers in Nerve, Muscle, and Glial Cells. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9685-8_1
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