Relationships between NADPH Content and Benzo(a)pyrene Metabolism in Normal and Glucose-6-Phosphate Dehydrogenase-Deficient Human Fibroblasts

  • Rosa Pascale
  • Lucia Daino
  • Maria E. Ruggiu
  • Maria G. Vannini
  • Renato Garcea
  • Serenella Frassetto
  • Luciano Lenzerini
  • Leonardo Gaspa
  • Maria M. Simile
  • Marco Puddu
  • Francesco Feo

Abstract

Previous work in our laboratory has shown that human skin fibroblasts (HSF) and human lymphocytes carrying the Mediterranean variant of glucose-6-phosphate dehydrogenase (G6PD) exhibit a great decrease in hexose monophosphate shunt and in NADP /NADPH ratio1,2. G6PD deficiency protects in vitro growing HSF and lymphocytes from benzo(a)pyrene (BaP) toxicity. G6PD-deficient HSF give rise in soft agar, after incubation with BaP, to a lower number of colonies than normal HSF1. Aryl hydrocarbon hydroxylase (AHH) activities are lower in G6PD-deficient cells, when tested in the absence of exogenous NADPH2. G6PD-deficient cells, incubated in vitro with BaP, produce low amounts of organic- and water-soluble BaP metabolites and show a decreased ability to form BaP-7,8-diol-9,10-epoxide and BaP-DNA adducts3,4.

Keywords

G6PD Deficiency Deficient Cell Polar Fraction Human Skin Fibroblast Mouse Embryo Fibroblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Rosa Pascale
    • 1
  • Lucia Daino
    • 1
  • Maria E. Ruggiu
    • 1
  • Maria G. Vannini
    • 1
  • Renato Garcea
    • 1
  • Serenella Frassetto
    • 1
  • Luciano Lenzerini
    • 1
  • Leonardo Gaspa
    • 1
  • Maria M. Simile
    • 1
  • Marco Puddu
    • 1
  • Francesco Feo
    • 1
  1. 1.Istituto di Patologia generaleUniversità di SassariSassariItaly

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