Advertisement

Lipid Peroxidation and Bioactivation of Halogenated Hydrocarbons in Rat Liver Mitochondria During Experimental Siderosis

  • Aldo Tomasi
  • Emanuele Albano
  • Barbara Botti
  • Francesco P. Corongiu
  • M. Assunta Dessì
  • Anna Iannone
  • Valeria Franceschi
  • Vanio Vannini
  • Alberto Masini
Chapter

Abstract

It is firmly extablished that increased amount of iron accumulated in hepatic parenchymal cells is associated with tissue injury, fibrosis and ultimately, cirrhosis1. However, the pathogenetic mechanism of iron in determining the liver injury has not been experimentally proven2. Currently two hypothesis have been put forward in order to explain the hepatocellular injury in chronic iron overload. The first implies that the excess iron largely occurring in lysosomes, physically disrupts these organelles with the release of cell damaging hydrolytic enzymes3. The second one presupposes that the pathological accumulation of iron elicits membrane lipid peroxidation in cellular organelles resulting in structural and functional alterations of cell integrity4. Indeed, iron could promote the production of reactive oxygen species such as Superoxide anion (O·− 2), hydroxy radicals (OH·− 4), and hydrogen peroxide (H2O2)5. The experimental evidence, gathered up to now, indicates that chronic iron overload may induce in vivo lipid peroxidation of mitochcndrial membranes6–8. Furthermore, the in vivo occurrence of lipid peroxidation in the mitochondrial membranes has been suggested to be responsible for some anomalies in liver mitochondria isolated from rats made siderotic either by dietary iron9,10 or by intraperitoneal injection of iron-(III)- gluconate complex11–13.

Keywords

Electron Spin Resonance Iron Overload Liver Mitochondrion Carbonyl Iron Hepatic Iron Overload 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    L. Vf. Powell, Hemochromatosis and related iron storage disease in: “Liver and Biliary Disease”, R. Wright, G. H. Millward-Sandier, K. G. M. M. Alberti and S. Korran, eds., Bailliere Tindall Saunders Company, London (1985).Google Scholar
  2. 2.
    A. Jacobs, The pathology of iron overload, in: “Iron in Biochemistry and Medicine”, A. Jacobs and M. Woorwood, eds., Academic Press, London (1980).Google Scholar
  3. 3.
    T. J. Peters and C. A. Seymour, Acid hydroxylase activities and lysosomal integrity in liver biopsies from patients with iron overload, Clin. Sci Mol Med. 50:75 (1976).PubMedGoogle Scholar
  4. A. B. Halliwell and J. M. C. Gutteridge, The importance of free radicals and catalytic metal ions in human disease, Molee. Aspects Med. 8:89 (1985).CrossRefGoogle Scholar
  5. 5.
    B. Halliwell and J. M. C. Gutteridge, Oxygen toxicity, oxygen radicals, transition metals and disease, Biochem. J. 219:1 (1984).PubMedGoogle Scholar
  6. 6.
    G. Hanstein, D. T. Heitmann, A. Sandy, H. L. Biesterfeld, H. H. Liem and U. Muller-Eberhard, Effects of hexachlorobenzene and iron loading on rat liver mitochondria, Biochim Biophys Acta 678:293 (1981).PubMedCrossRefGoogle Scholar
  7. 7.
    B. R. Bacon, G. M. Brittenham, A. S. Tavill, C. E. McLaren, H. Park and R. O. Recknagel, Hepatic lipid peroxidation in vivo in rats with chronic dietary iron overload is dependent on hepatic iron concentration, Trans. Assoc. Amer. Phys. 96:146 (1983).Google Scholar
  8. 8.
    B. R. Bacon, A. S. Tavill, G. M. Brittenham, C. H. Park and R. O. Recknagel, Hepatotoxicity of chronic iron overload: Role of lipid peroxidation, in: “Free Radicals in Liver Injury”, G. Poli, K. H. Cheeseman, M. U. Dianzani and T. F. Slater, eds., IRL Press, Oxford (1985).Google Scholar
  9. 9.
    B. R. Bacon, A. S. Tavill, G. M. Brittenham, C. H. Park and R. O. Recknagel, Hepatic lipid peroxidation in vivo in rats with chronic iron overload, J, Clin. Invest. 71:429 (1983).CrossRefGoogle Scholar
  10. 10.
    B. R. Bacon, C. H. Park, G. M. Brittenham, R. O’Neil and A. S. Tavill, Hepatic mitochondrial oxidative metabolism in rats with chronic dietary iron overload, Hepatology 5:789 (1985).PubMedCrossRefGoogle Scholar
  11. 11.
    T. Trenti, A. Masini, D. Ceccarelli-Stanzani, E. Rocchi and E. Ventura, Functional efficiency of mitochondrial membrane of rats with hepatic iron overload, Hepatology 4:777 (1984).Google Scholar
  12. 12.
    A. Masini, T. Trenti, E. Ventura, D. Ceccarelli-Stanzani and U. Muscatello, Functional efficiency of mitochondrial membrane of rats with hepatic chronic iron overload, Biochem. Biophys. Res. Commun. 124:462 (1984).PubMedCrossRefGoogle Scholar
  13. 13.
    A. Masini, D. Ceccarelli-Stanzani, T. Trenti and E. Ventura, Transmembrane potential of liver mitochondria from hexachlorobenzene and iron treated rats, Biochim. Biophys. Acta 802:253 (1984).PubMedCrossRefGoogle Scholar
  14. 14.
    A. Tomasi, E. Albano, S. Banni, B. Botti, F. Corongiu, M. A. Dessi, A. Iannone, V. Vannini and M. U. Dianzani, Free-radical metabolism of carbon tetrachloride in rat liver mitochondria, Biochem. J. 246:313 (1987).PubMedGoogle Scholar
  15. 15.
    A. Masini, D. Ceccarelli-Stanzani and U. Muscatello, The effect of oligomycin in rat liver mitochondria respiring in state 4, FEBS Lett. 160:137 (1984).Google Scholar
  16. 16.
    F. P. Corongiu, M. Lai and A. Milia, Carbon tetrachloride, bromotrichloromethane and ethanol acute intoxication, Biochem. J. 212:625 (1983).PubMedGoogle Scholar
  17. 17.
    F. P. Corongiu, M. A. Dessi, S. Vargiolu, G. Poli, K. H. Cheeseman, M. U. Dianzani and T. F. Slater, Antioxidant activity of a-tocopherol against lipid peroxidation in rat liver microsomes, in: “Free Radicals in Liver Injury”, G. Poli, K. H. Cheeseman, M. U. Dianzani and T. F. Slater, eds., IRL Press, Oxford (1985).Google Scholar
  18. 18.
    B. Chance and G. R. Williams, The respiratory chain and oxidative phosphorylation, Adv. Enzymol. 17:65 (1956).Google Scholar
  19. 19.
    H. R. Lotscher, K. H. Winterhalter, E. Carafoli and C. Richter, The energy state of mitochondria during the transport of Ca, Eur. J. Biochem. 110:211 (1980).PubMedCrossRefGoogle Scholar
  20. 20.
    G. Poli, E. Gravela, E. Albano and M. U. Dianzani, Studies on fatty liver with isolated hepatocytes, Exp. Mol. Pathol. 30:116 (1979).PubMedCrossRefGoogle Scholar
  21. 21.
    E. Albano, K. A. K. Lott, T. F. Slater, A. Stier, M. C. R. Symons and A. Tomasi, Spin trapping studies on the free radical products formed by metabolic activation of carbon tetrachloride in rat liver microsomal fractions isolated hepatocytes and in vivo in the rat, Biochem. J. 204:593 (1982).PubMedGoogle Scholar
  22. 22.
    H. L. Bonkowsky, J. F. Healey, P. R. Sinclair, J. F. Sinclair and J. S. Pomeroy, Iron and the liver. Acute and long term effect of iron loading on hepatic heme metabolism, Biochem. J. 196:57 (1981).PubMedGoogle Scholar
  23. 23.
    R. T. Parmley, M. E. May, S. S. Spier, M. G. Buse and C. J. Alvarez, Ultrastructural distribution of inorganic iron in normal and iron loaded hepatic cell, Lab. Invest. 44:475 (1981).PubMedGoogle Scholar
  24. 24.
    A. Masini, T. Trenti, D. Ceccarelli and U. Muscatello, Mitochondrial involvement in causing cell injury in experimental hepatic iron overload,Ann. NY Acad. Sci. 488:517 (1986).CrossRefGoogle Scholar
  25. 25.
    A. Masini, T. Trenti, E. Ventura, D. Ceccarelli and U. Muscatello, The effect of ferric iron complex oh Ca2+ transport in isolated rat liver mitochondria, Biochem. Biophys. Res. Commun. 130:207 (1985).PubMedCrossRefGoogle Scholar
  26. 26.
    A. Masini, T. Trenti, D. Ceccarelli-Stanzani and E Ventura, The effect of ferric iron complex on isolated rat liver mitochondria. I. Respiratory and electrochemical responses, Biochim. Biophys. Acta 810:20 (1985).PubMedCrossRefGoogle Scholar
  27. 27.
    A. Masini T. Trenti, D. Ceccarelli-Stanzani and E. Ventura, The effect of ferric iron complex on isolated rat liver mitochondria. II. Ion movements, Biochim. Biophys. Acta 810:27 (1985).PubMedCrossRefGoogle Scholar
  28. 28.
    A. Masini, T. Trenti, D. Ceccarelli and U. Muscatello, The effect of ferric iron complex on isolated rat liver mitochondria. III. Mechanistic aspects of iron induced calcium efflux, Biochim. Biophys. Acta 891:150 (1987).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Aldo Tomasi
    • 1
  • Emanuele Albano
    • 1
    • 2
  • Barbara Botti
    • 1
  • Francesco P. Corongiu
    • 1
    • 3
  • M. Assunta Dessì
    • 1
    • 3
  • Anna Iannone
    • 1
  • Valeria Franceschi
    • 1
  • Vanio Vannini
    • 1
  • Alberto Masini
    • 1
  1. 1.Istituto di Patologia GeneraleModenaItaly
  2. 2.Dipartimento di Medicina a Oncologia SperimentaleTorinoItaly
  3. 3.Dipartimento di Biologia SperimentaleSezione di Patologia SperimentaleCagliariItaly

Personalised recommendations