In Situ Detection of Hepatic AF-DNA Adduct Formation During Continuous Feeding of 0.02% Acetylaminofluorene and in Enzyme-Altered Foci Induced by Foyr Different Protocols

  • Henrik S. Huitfeldt
  • Henry C. Pitot
  • John M. Hunt
  • Jeffrey Baron
  • Miriam C. Poirier


Formation of carcinogen-DNA adducts is thought to represent the critical event in the initiation of tumorigenesis in many carcinogenesis models. DNA-adduct formation and repair has therefore been profoundly studied, both for human monitoring of carcinogen exposure and in experimental carcinogenesis. Most studies on DNA-adducts are based upon extraction of DNA from whole tissue, with little possibility to distinguish between different cell populations. The ability to differentiate between subpopulations within the tissue is presumably important, both to assess adduct processing within the tumor progenitor cells, and to monitor biological alterations of initiated cell populations.


Adduct Formation Liver Lobule Continuous Feeding Bile Duct Proliferation Bile Duct Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    H. S. Huitfeldt, E. F. Spangler, J. M. Hunt and M. C. Poirier, Immunohistochemical localization of DNA adducts in rat liver tissue and phenotypically altered foci during oral administration of 2-acetylaminofluorene, Carcinogenesis 7:123 (1986).PubMedCrossRefGoogle Scholar
  2. 2.
    M. C. Poirier, J. Nakayama, F. P. Perera, I. B. Weinstein and S. H. Yuspa, Identification of carcinogen-DNA adducts by immunoassays, in: “Application of Biological Markers to Carcinogen Testing”, H. A. Milman and S. Sell, eds., Plenum Press, New York (1983).Google Scholar
  3. 3.
    H. Holthofer, A. Miettinen, R. Paasivuo, V. P. Lehto, E. Linder, O. Alfthan and I. Virtanen, Cellular origin and differentiation of renal carcinomas. A fluorescence microscopic study with kidney-specific antibodies, anti-intermediate filament antibodies and lectins, Lab. Invest. 49:317 (1983).PubMedGoogle Scholar
  4. 4.
    F. A. Beland, H. S. Huitfeldt and M. C. Poirier, DNA adduct formation and removal during chronic administration of a carcinogenic aromatic amine, Prog. exp. Tumor Res. 31:33 (1987).PubMedGoogle Scholar
  5. 5.
    H. S. Huitfeldt, E. F. Spangler, J. Baron and M. C. Poirier, Microfluorometric determination of DNA adducts in immunofluorescent-stained liver tissue from rats fed 2-acetylaminofluorene, Cancer Res. 47:2098 (1987).PubMedGoogle Scholar
  6. 6.
    H. C. Pitot, L. Barsness, T. Goldsworthy and T. Kitagawa, Biochemical characterization of stages of hepatocarcinogenesis after a single dose of diethylnitrosamine, Nature 272:456 (1978).CrossRefGoogle Scholar
  7. 7.
    H. A. Campbell, H. C. Pitot, V. R. Potter and B. A. Laishes, Application of quantitative stereology to the evaluation of enzyme-altered foci in rat liver, Cancer Res. 41:465 (1982).Google Scholar
  8. 8.
    T. L. Goldsworthy and H. C. Pitot, The quantitative analysis and stability of histochemical markers of altered hepatic foci in rat liver following initiation by diethylnitrosamine and promotion with phenobarbital, Carcinogenesis 6:1261 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    H. P. Glauert, D. Beer, M. Sambasiva Rao, M. Schwarz, Y-D. Xu, T. L. Goldsworthy and H. C. Pitot, Induction of altered hepatic foci in rats by the administration of hypolipidemic peroxisome proliferators alone or following a single dose of diethylnitrosamine, Cancer Res. 46:4601 (1986).PubMedGoogle Scholar
  10. 10.
    J. M. Hunt, M. T. Buckley, B. A. Laishes and H. A. Dunsford, Immunological approaches to the purification of putative premalignant hepatocytes from genotypic mosaic rat livers, Cancer Res, 45:2226 (1985).PubMedGoogle Scholar
  11. 11.
    S. Hendrich, H. P. Glauert and H. C. Pitot, The phenotypic stability of altered hepatic foci: effects of withdrawal and subsequent readministration of phenobarbital, Carcinogenesis 7:2041 (1986).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Henrik S. Huitfeldt
    • 1
  • Henry C. Pitot
    • 2
  • John M. Hunt
    • 3
  • Jeffrey Baron
    • 4
  • Miriam C. Poirier
    • 5
  1. 1.Laboratory for Immunohistochemistry and Immunopathology Institute of PathologyThe National Hospital, University of OsloNorwayUSA
  2. 2.McArdle Laboratory for Cancer ResearchUniversity of WisconsinMadisonUSA
  3. 3.Department of Pathology and Laboratory MedicineUniversity of Texas Medical SchoolHoustonUSA
  4. 4.Department of Pharmacology, College of MedicineUniversity of IowaIowa CityUSA
  5. 5.Laboratory of Cellular Carcinogenesis and Tumor Promotion National Cancer InstituteNIHBethesdaUSA

Personalised recommendations