Cytochrome P-450 Function Analyses with Monoclonal Antibodies and cDNA Expression Vectors

  • Harry V. Gelboin
  • Frank J. Gonzalez
  • Sang S. Park
  • Junji Sagara
  • Narayana Battula


It is quite fitting and proper to initiate this International Conference devoted to the problems of chemical carcinogenesis with a discussion of the role and function of cytochrome P-450. Cytochrome P-450 is the enzymatic interface between mammalian organisms, including man, and a large variety of foreign chemicals, i.e. xenobiotics, which include almost all chemical carcinogens.


Aniline Hydroxylase cDNA Expression Vector Microsomal Monooxygenase System Aniline Hydroxylation Drug Nifedipine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    H. V. Gelboin and F. K. Friedman, Monoclonal antibodies for studies on xenobiotic and endobiotic metabolism. Cytochromes P-450 as paradigm, Biochem. Pharmacol. 34:2225 (1985).CrossRefPubMedGoogle Scholar
  2. 2.
    S. S. Park, T. Fujino, D. K. West, F. P. Guengerich and H. V. Gelboin, Monoclonal Antibodies that Inhibit Enzyme Activity of 3-Methylcholanthrene-induced Cytochrome P-450, Cancer Res. 42:1798 (1982).PubMedGoogle Scholar
  3. 3.
    S. S. Park, T. Fujino, H. Miller, F. P. Guengerich and H. V. Gelboin, Monoclonal antibodies to phenobarbital-induced rat liver cytochrome P-450, Biochem. Pharmacol. 33:2071 (1984).CrossRefPubMedGoogle Scholar
  4. 4.
    I. Y. Ko, S. S. Park, B. J. Song, C. Patten, Y. Tan, Y. C. Hah, C. C. Yang and H. V. Gelboin, Monoclonal Antibodies to Ethanol-induced Rat liver Cytochrome P-450 That Metabolized Aniline and Nitrosamines, Cancer Res. 47:3101 (1987).PubMedGoogle Scholar
  5. 5.
    S. S. Park, D. J. Waxman, H. Miller, R. Robinson, C. Attisano, F. P. Guengerich and H. V. Gelboin, Preparation and characterization of monoclonal antibodies to pregnenolone 16-alpha-carbonitrile inducible rat liver cytochrome, Biochem. Pharmacol. 35:2859 (1986).CrossRefPubMedGoogle Scholar
  6. 6.
    T. Fujino, S. S. Park, D. West and H. V. Gelboin, Phenotyping of cytochromes P-450 in human tissues with monoclonal antibodies, Proc. Natl. Acad. Sci. 70:3682 (1982).CrossRefGoogle Scholar
  7. 7.
    T. Fujino, D. West, S. S. Park and H. V. Gelboin, Monoclonal antibodydirected phenotyping of cytochrome P-450-dependent aryl hydrocarbon hydroxylase and 7-ethoxycoumarin deethylase in mammalian tissues, J. Biol. Chem. 259:9044 (1984).PubMedGoogle Scholar
  8. 8.
    E. Hietanen, C. Malaveille, F. K. Friedman, S. S. Park, J. C. Bereziat, G. Brun, H. Bartsch and H. V. Gelboin, Monoclonal Antibody-directed Analysis of Cytochrome P-450-dependent Monooxygenases and Mutagen Activation in the Livers of DBA/2 and C57BL/6 Mice, Cancer Res. 46:524 (1986).PubMedGoogle Scholar
  9. 9.
    J. R. Idle and R. L. Smith, Polymorphisms of oxidation at carbon centers of drugs and their clinical significance, Drug Metabol. Rev. 9:301 (1979).CrossRefGoogle Scholar
  10. 10.
    R. P. Peng, P. Tennant, N. A. Lorr and C. S. Yang, Alterations of microsomal monooxygenase system and carcinogen metabolism by streptozotocin-induced diabetes in rats, Carcinogenesis 4:703 (1983).CrossRefPubMedGoogle Scholar
  11. 11.
    N. Battula, J. Sagara and H. V. Gelboin, Expression of P1-450 and P3-450 coding sequences as enzymatically active cytochromes P-450 in mammalian cells, Proc. Natl. Acad. Sci. 84:4073 (1987).CrossRefPubMedGoogle Scholar
  12. 12.
    B. J. Song, H. V. Gelboin, S. S. Park, C. S. Yang and F. J. Gonzalez, Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s. Transcriptional and post-transcriptional regulation of the rat enzyme, J. Biol. Chem. 261:16689 (1986).PubMedGoogle Scholar
  13. 13.
    B. J. Song, T. Matsunaga, J. P. Hardwick, S. S. Park, R. L. Veech, C. S. Yang, H. V. Gelboin and F. J. Gonzalez, Stabilization of cytochrome P450J mRNA in teh diabetic rat, Molec. Endocrinol. in press (1987).Google Scholar
  14. 14.
    O. W. McBride, M. V. Umeno, H. V. Gelboin and F. J. Gonzalez, A TaqI polymorphysm in the human P450 IIE1 gene on chromosome 10, Nucleic Ac. Res. in press (1987).Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Harry V. Gelboin
    • 1
  • Frank J. Gonzalez
    • 1
  • Sang S. Park
    • 1
  • Junji Sagara
    • 1
  • Narayana Battula
    • 1
  1. 1.Laboratory of Molecular CarcinogenesisNational Cancer Institute, National Institutes of HealthBethesdaUSA

Personalised recommendations