Design and Development of Enzymatic Organic Synthesis
The rate acceleration and selectivity of various enzymatic reactions operated under mild conditions are the major attractive features of enzymes for use as catalysts in organic synthesis. Many natural and unnatural enzymatic reactions have been demonstrated for multigram scale synthesis of chiral organic molecules1. The number of enzymes isolated (about 2,500), however, only represents approximately 2% of the total number of enzymes which may exist in nature. So far, there have been only about 50 enzymes exploited for use in organic synthesis. With an increasing number of enzymes available, the synthetic methods based on enzyme catalysis are being extended from the preparation of small chiral molecules to the synthesis of more complex molecules such as oligosaccharides, polypeptides, nucleotides and their conjugates. This review describes the most recent developments in my laboratory in this area with emphasis on the synthesis of carbohydrates and polypeptides.
KeywordsAldol Reaction Dihydroxyacetone Phosphate Amidase Activity Diethyl Acetal Amide Hydrolysis
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