Effect of Metal Ions and Adenylylation State on the Energetics of the E. Coli Glutamine Synthetase Reaction

  • Lynn M. Abell
  • Joseph J. Villafranca
Part of the Industry-University Cooperative Chemistry Program Symposia book series (IUCC)


Glutamine synthetase (GS) from E. coli requires two divalent metal ions per active site in order to catalyze the ATP-dependent formation of glutamine from glutamate and ammonia and plays a central role in ammonia metabolism in both prokaryotic and eukaryotic systems.


Glutamine Synthetase Association Rate Internal Equilibrium Burst Rate Biosynthetic Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



glutamine synthetase

GS n=2

low adenylylation state enzyme

GS n=12

high adenylylation state enzyme


N-(2-hydroxyethyl)piperazine-N′ -2-ethanesulfonic acid


phosphoenol pyruvate


ethylene diamine tetraacetic acid


piperazine-N, N′ -bis(2-ethanesulfonic acid)


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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Lynn M. Abell
    • 1
  • Joseph J. Villafranca
    • 1
  1. 1.Department of ChemistryThe Pennsylvania State UniversityUniversity ParkUSA

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