Expression of Dipeptidylpeptidase IV (DPP IV/CD26) Activity on Human Myeloid and B Lineage Cells, and Cell Growth Suppression by the Inhibition of DPP IV Activity
Human CD26 antigen is a transmembrane glycoprotein with dipeptidyl peptidase IV (DPP IV) activity in its extracellular domain. Within the hematopoietic system, CD26/DPP IV has been thought to be exclusively expressed in activated T and NK cells . However, by enzymatic assay, Bauvois et al. have characterized DPP IV activity (sensitive to diprotin A which is the specific inhibitor of DPP IV) present on the surface of human myeloblastic HL-60 and monoblastic U937 cell lines as well as on peripheral monocytes, granulocytes and macrophages . Moreover, DPP IV activity increased strictly on cells undergoing macrophage maturation . Reinhold et al. found that synthetic reversible inhibitors of DPP IV suppressed cell proliferation and modulated cytokine production in U937 cells expressing high levels of CD26 . Monoclonal antibody (mAb) anti-Tal (purchased from Coulter, Hialeah, FL) and directed against CD26 of activated human T cells was recently shown to react with a few B cell lines including Daudi cells which are tumor cells derived from Burkitt lymphoma and EBV-transformed cells . Another anti-CD26 mAb i.e. anti-TA5.9 (from Eurogenetics, Tessenderlo, Belgium) was shown to bind activated T cells as well as myeloid cells, Raji and U266 cells .
KeywordsU937 Cell Dipeptidyl Peptidase Peripheral Monocyte Diphenyl Phosphate Human Myeloid Cell
Unable to display preview. Download preview PDF.
- 2.Bauvois B, J. Sancéau J, Wietzebin J (1992) Human U937 cell surface peptidase activities: characterization and degradative effect on tumor necrosis factor-a. Eur. J. Immunol. 22: 923–930.Google Scholar
- 5.Shaw S, Gilks W. (1995). In: White Cell Differentiation Antigens, Schlossman SF, Boumsell L, Golks W, Harlan JM, kishimoto T, Morimoto C, Ritz J, Shaw S, Silverstein R, Springer T, Tedder TF, Todd RF eds. Leukocyte Typing V. Oxford University Press, Oxford-New York-Tokyo: pp 78.Google Scholar
- 6.Lambeir AM, Borloo M, De Meester 1, Belyaev A, Augustyns Khendriks D, Scharpé S, Haemers A. (1996) Dipeptide-derived diphenyl phosphate ester: mechanism-based inhibitors of dipeptidyl peptidase IV. Biochem. Biophvs Acta. 1290: 76–82.Google Scholar
- 7.Schön E, Jahn S, Kiessig T, Demuth HU, Neubert K, Barth A, Von Baehr R, Ansorge S. (1987) The role of dipeptidylpeptidase IV in human T lymphocyte activation inhibitors and antibodies against dipeptidylpeptidase IV suppress lymphocyte proliferation and immunoglobulin synthesis in vitro. Eur J hnmunol. 17: 1821–1826.CrossRefGoogle Scholar
- Reinhold D, Bank DU. Böhling F, Neubert K, Mattem T, Ulmer AJ, Flad HD, Ansorge S. (1993) Dipeptidylpeptidase IV (CD26) on human lymphocytes. Synthetic inhibitors of and antibodies against dipeptidylpeptidase IV suppress the proliferation of pokeweed mitogen-stimulated peripheral blood mononuclear cells and IL-2 and IL-6 production. Immunohiol. 188: 403–409.Google Scholar