New Fluorogenic Dipeptidyl Peptidase IV/CD26 Substrates and Inhibitors

  • Susan Lorey
  • Jürgen Faust
  • Uta Hermanns
  • Frank Bühling
  • Siegfried Ansorge
  • Klaus Neubert
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 421)

Abstract

Dipeptidyl peptidase IV (DP IV, CD26) is a membrane bound serine protease which ubiquitously occurs in epithelial tissues of mammalian organisms, above all in kidney, liver and intestine1,2 but also on the surface of immune cells3. DP IV is involved not only in activation of B- and T-lymphocytes4,5 and NK-cells6 with peptidase activity but also in the regulation of growth process, DNA-synthesis and cytokine production7. Therefore it is important for the signal transduction during the immune response. DP IV catalyses the cleavage of N-terminal dipeptides from oligo- and polypeptides where the penultimate residue is proline or -with less efficiency- alanine8,9. To investigate the physiological role of DP IV in further detail, specific high sensitive substrates or inhibitors would be useful tools. Here we describe the synthesis and enzymatic characterisation of sensitive fluoro-genic rhodamine 110 (R110)-based substrates and fluoresceine- or biotin-labelled inhibitors and their use for the sensitive detection of DP IV on the surface of T-cell lines.

Keywords

Kinetic Constant Human Lymphocyte Dipeptidyl Peptidase Fluorogenic Substrate Human Natural Killer Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Susan Lorey
    • 1
  • Jürgen Faust
    • 1
  • Uta Hermanns
    • 1
  • Frank Bühling
    • 2
  • Siegfried Ansorge
    • 2
  • Klaus Neubert
    • 1
  1. 1.Department of Biochemistry and Biotechnology Institute of BiochemistryMartin-Luther-University Halle-WittenbergHalleGermany
  2. 2.Institute of Experimental Internal Medicine Center of Internal MedicineOtto-von-Guericke-University MagdeburgGermany

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