The Effect of Anti-CD26 Antibodies on DNA Synthesis and Cytokine Production (IL-2, IL-10 and IFN-γ) Depends on Enzymatic Activity of DP IV/CD26

  • D. Reinhold
  • T. Kähne
  • M. Täger
  • U. Lendeckel
  • F. Bühling
  • U. Bank
  • S. Wrenger
  • J. Faust
  • K. Neubert
  • S. Ansorge
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 421)


Dipeptidyl peptidase IV (DP IV, EC is a transmembrane type II glycoprotein present on most mammalian cells 1,2.


Adenosine Deaminase Phorbol Myristate Acetate Dipeptidyl Peptidase Dipeptidyl Aminopeptidase Transmembrane Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Fleischer, B. (1994) CD26: a surface protease involved in T-cell activation../. ImnumoL 15, 180–184.Google Scholar
  2. 2.
    Morimoto, C. Schlossman. S. F. 11994) A key costimulatory molecule on CD4 memory T cells. The Immunologist 2, 4- -7.Google Scholar
  3. 3.
    Bühling, F., Junker, U., Reinhold, D.. Neubert, K., Jäger, L. Ansorge, S. (1995) Functional role of CD26 on human B lymphocytes. Immunol. Lett. 45, 47–51.PubMedCrossRefGoogle Scholar
  4. 4.
    Bühling, F.. Kunz. D., Reinhold, D., Ulmer, A. J., Ernst, M., Flad, H.-D. Ansorge, S. (1994) Expression and functional role ofdipeptidyl peptidase IV on human natural killer cells. Nat. Immun. 13, 270–279.PubMedGoogle Scholar
  5. 5.
    Yaron, A. Naider, F. (1993) Proline-dependent structural and biological properties of peptides and proteins. Olt. Rev Biochem. Mol. Biol. 28, 31–81.CrossRefGoogle Scholar
  6. 6.
    Ulmer, A. J., Mattern, T., Feller, A. C., Heymann, E. Flad, H.–D. (1990) CD26 antigen is a surface Dipeptidyl peptidase IV (DPPIV) as characterised by monoclonal antibodies clone TII–l9–4–7 and 4ELICl. Scand. J. Immunol. 31, 429 – 435.Google Scholar
  7. 7.
    Hegen, M., Niedobitek, C., Klein, C. E., Stein, H. Fleischer, B. (1990) The T cell triggering molecule Tp103 is associated with Dipeptidyl aminopeptidase IV activity. J. Immunol. 144, 2908–2914.Google Scholar
  8. 8.
    Mattern, T., Flad, H.-D., Feller, A. C., Heymann, E. Ulmer, A. J. (1989) Anti-DPP-IV and anti-Tal but not IOT 15 should be clustered in CDw26. In: Leukocyte Typing IV. Knapp, W., Dörken, B., Gilks, W. R., Rieber, E. P., Schmidt, R. E., Stein, H. Von Dem Borne, A. E. G. K. (Eds.), 416.Google Scholar
  9. 9.
    Kameoka, J., Tanaka, T., Nojima, Y., Schlossman, S. F. Morimoto, C. (1993) Direct association of adenosine deaminase with a T cell activation antigen, CD26. Science 261, 466–469Google Scholar
  10. 10.
    Morimoto, C., Torimoto, Y., Levinson, G., Rudd, C. E., Schrieber, M., Dang, N. H., Letvin, N. L. Schlossman, S. F. (1989) 1F7, a novel cell surface molecule, involved in helper function of CD4 cells. J. Immunol. 143, 3430–3439.Google Scholar
  11. 11.
    Fleischer, B., Sturm, E., De Vries, J. E. Spits, H. (1988) Triggering of cytotoxic T lymphocytes and NK cells via the Tp103 pathway is dependent on the expression of the T cell receptor’CD3 complex..1 Immunol. 141, 1103–1107.Google Scholar
  12. 12.
    Hegen, M., Camerini, D. Fleischer, B. (1993) Function of dipeptidyl peptidase IV (CD26, Tp103) in transfected human T cells. Cell. Immunol. 146, 249–260.Google Scholar
  13. 13.
    Ansorge, S., Schön, E. Kunz, D. (1991) Membrane-bound peptidases of lymphocytes: Functional imphcations. Biomed. Biochem. Acta 50, 799–807.Google Scholar
  14. 14.
    Schön, E., Demuth. U. H., Barth, A. Ansorge, S. (1984) Dipeptidyl peptidase IV of human lymphocytes. Evidence for specific hydrolysis of glycylprolin in T-lymphocytes. Biochem. J. 223, 255–258.Google Scholar
  15. 15.
    Schön. E., Eichmann, E., Grunow, R.. Jahn, S., Kiessig, S. T., Volk, H. D. Ansorge, S. (1986) Dipeptidyl peptidase IV in human T lymphocytes. An approach to the role of a membrane peptidase in the immune system. Biomed. Biochem. Acta 45, 1523–1528.Google Scholar
  16. 16.
    Dang, N. H., Torimoto, Y., Deusch, K., Schlossman, S. F. Morimoto, C. (1990) Comitogenic effect of solid-phase immobilized anti-I F7 on human CD4 T cell activation via CD3 and CD2 pathways,.1. Immunol. 144, 4092–4100.Google Scholar
  17. 17.
    Dang, N. H., Torimoto, Y., Sugita, K., Daley, J. F., Show, P., Porado, C., Schlossman, S. F. Morimoto, C (1990) Cell surface modulation of CD26 by anti-1F7 monoclonal antibody. Analysis of surface expression and human T cell activation. J. Imnumol. 145, 3963.Google Scholar
  18. 18.
    Dang, N. H., Torimoto, Y., Shimamura, K., Tanaka, T., Daley, J. F.. Schlossman, S F Morimoto, C. (1991) 1F7 (CD26): A marker of thymic maturation involved in the differential regulation of the CD3 and CD2 pathways of human thymocyte activation. J. Immunol 147, 2825–2832.Google Scholar
  19. 19.
    Schön, E., Born. I., Demuth, H.-U., Faust, J., Neubert, K., Stemmetzer, T., Barth, A. Ansorge, S. (1991) Dipeptidyl peptidase IV in the immune system. Effects of specific enzyme inhibitors on activity of dipeptidyl peptidase IV and proliferation of human lymphocytes. Biol. Chem. Hoppe Sevier 372, 305–311.Google Scholar
  20. 20.
    Schön. E., Demuth, H.-U., Eichmann, E., Horst, H.-J., Körner, 1.-.1., Kopp, J.. Mattem, T., Neubert, K., Noll, F., Ulmer, J. A., Barth, A. Ansorge, S. (1989) Dipeptidyl peptidase IV in human T lymphocytes Impaired induction of interleukin 2 and gamma interferon due to specific inhibition of dipeptidyl peptidase IV. Scand. J. Immunol. 29, 127–132.CrossRefGoogle Scholar
  21. 21.
    Schön, E., Jahn, S., Kiessig, S. T., Demuth, H.-U., Neubert, K., Barth, A., von Baehr, R. Ansorge. S. (1987) The role of dipeptidyl peptidase IV in human T lymphocyte activation. inhibitors and antibodies against dipeptidyl peptidase IV suppress lymphocyte proliferation and immunoglobulin synthesis in vitro. Eue J. Immunol. 17, 1821–1826.CrossRefGoogle Scholar
  22. 22.
    Torimoto, Y., Dang, N. H., Vivier, E., Tanaka, T.. Schlossman, S. F. Morimoto.. C (1991) Coassocìation of CD26 (dipeptidyl peptidase IV) with CD45 on the surface of human T lymphocytes.1. Immunol_ 147. 2514–2517.Google Scholar
  23. 23.
    Torimoto, Y., Dang, N. H., Tanaka, T., Prado, C., Schlossman, S. F Morimoto, C. (1992) Biochemical characterization of CD26 (dipeptidyl peptidase IV): Functional comparison of distinct epitopes recognized by various anti-CD26 monoclonal antibodies. Mol. Immunol. 29, 183–192.PubMedCrossRefGoogle Scholar
  24. 24.
    Schön, E., Eichmann, E., Jahn, S., Kopp, J., Volk, H. D. Ansorge, S. (1988) On the role of dipeptidyl peptidase IV (DP IV) in thew immune system. Relations to the cell cycle and lymphokine production of activated lymphocytes. Biol. Zent. bl. 107, 141–149.Google Scholar
  25. 25.
    Reinhold, D., Bank. U., Bühling, F., Neubert, K., Mattem, T., Ulmer, A. J., Flad, H.-D. Ansorge, S. (1993) Dipeptidyl peptidase IV (CD26) on human lymphocytes. Synthetic inhibitors of and antibodies against Dipeptidyl peptidase IV suppress the proliferation of pokeweed mitogen-stimulated peripheral blood mononuclear cells and IL-2 and IL-6 production. Immunobiol. 188, 403–414.Google Scholar
  26. 26.
    Reinhold, D., Bank, U., Bühling, F., Kähne, T., Kunz, D., Faust, J., Neubert, K. Ansorge, S. (1994) Inhibitors of Dipeptidyl peptidase 1V (DP IV, CD26) specifically auppress proliferation and modulate cytokine production of strongly CD26 expressing U937 cells. bnmunobiology 192, 121–136.CrossRefGoogle Scholar
  27. 27.
    Fleischer, B. (1987) A novel pathway of human T cell activation via a 103 kD T cell activation antigen. J. Immunol. 138, 1346–1350.PubMedGoogle Scholar
  28. 28.
    Ansorge, S., Bühling, F., Hoffmann, T., Kähne, T., Neubert, K. Reinhold, D. (1995) DP IV/CD26 on human lymphocytes: Functional roles in cell growth and cytokine regulation. In: Dipeptidyl Peptidase IV (CD26) in metabolism and the immune response. Fleischer, B. (Eds.)R.G. Landes Company, Austin, (10)163–184.Google Scholar
  29. 29.
    Kähne, T., Kröning, H., Thiel. U., Ulmer, A. J., Flad, H.-D. Ansorge, S. (1996) Alterations in structure and cellular localization of molecular forms of DP IV/CD26 during T cell activation. Cell Immunol. 170. 63–70.PubMedCrossRefGoogle Scholar
  30. 30.
    Kähne, T. Ansorge, S. (1994) Non-radioaktive labelling and immunoprecipitation analysis of leukocyte surface proteins using different methods of protein biotinylation. J. Immunol. Methods 168, 209–218.PubMedCrossRefGoogle Scholar
  31. 31.
    Kähne, T., Neubert. K. Ansorge, S. (1995) Enzymatic activity of DP IV/CD26 is involved in PMA-induced hyperphosphorylation of p56’°“.. Immunol. Lett. 46, 189–193.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • D. Reinhold
    • 1
  • T. Kähne
    • 1
  • M. Täger
    • 1
  • U. Lendeckel
    • 1
  • F. Bühling
    • 1
  • U. Bank
    • 1
  • S. Wrenger
    • 1
  • J. Faust
    • 2
  • K. Neubert
    • 2
  • S. Ansorge
    • 1
  1. 1.Institute of Experimental Internal Medicine Center of Internal MedicineOtto-von-Guericke University MagdeburgMagdeburgGermany
  2. 2.Department of Biochemistry and BiotechnologyMartin-Luther-University Halle-WittenbergHalle(Saale)Germany

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