Aminopeptidase N-Mediated Signal Transduction and Inhibition of Proliferation of Human Myeloid Cells

  • M. Löhn
  • C. Mueller
  • K. Thiele
  • T. Kähne
  • D. Riemann
  • J. Langner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 421)


Cell surface peptidases are involved in degradative processes of various peptides, in cell-cell communcation, in lymphocyte activation and in cell growth1,2,3,4. Aminopeptidase N (APN, CD 13), a ubiquitously occurring surface molecule of many tissues, among them human liver, kidneys, intestine, placenta, bone marrow or brain microvessels, acts with a relatively broad substrate specifity on peptides with N-terminal neutral amino acids 5. Within the haematopoietic system CD13 is localized on monocytes, granulocytes and activated T lymphocytes5,6,7,8.


U937 Cell Bath Solution Chelate Zinc Human Myeloid Cell Cell Surface Density 
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  1. 1.
    Brownlees, J., Williams, C.H. (1993) Peptidases. peptides, and the mammalian blood-brain barrier. J. Neurochem. 60, 793–803PubMedCrossRefGoogle Scholar
  2. 2.
    Kohno, H., Kanno, T. (1985) Properties and activities of aminopeptidases in normal and mitogen stimulated human lymphocytes. J.Biochem. 226, 59–65Google Scholar
  3. 3.
    Kohno, H., Kanno, T. (1985) Intracellular localization and properties of aminopeptidases in human peripheral blood lymphocytes. Int. J. Biochem. 17, 1143–1148Google Scholar
  4. 4.
    Taylor, A. (1993) Aminopeptidases: towards a mechanism of action. TIBS 18, 167–172PubMedGoogle Scholar
  5. 5.
    Sanderink, G.-J., Artur,Y., Siest, G. (1988) Human aminopeptidase: A review of the literature. J. Clin Chem. Biochem. 26. 795–807Google Scholar
  6. 6.
    Riemann, D., Göhring. B., Langner, J. (1994) Expression of aminopeptidase N/CD13 in tumor-infiltrating lymphocytes from human renal cell carcinoma. Immunol. Lett. 42, 19–23Google Scholar
  7. 7.
    Razak, K., Newland, A.C. (1992) The significance of aminopeptidase and haematopoetic cell differentation. Blood Rev. 6, 243–250PubMedCrossRefGoogle Scholar
  8. 8.
    Look, A.T. (1989) Human myeloid plasma membrane glycoprotein CD13 (gp 150) is identical to aminopeptidase N. J.Clin.lnvest. 83, 1299–1307CrossRefGoogle Scholar
  9. 9.
    Mattern, T., Ansorge, S., Flad, H-D., Ulmer, A. (1993) Anti-CD26 monoclonal antibodies can reversibly arrest human T lymphocytes in the late Gi phase of the cell cycle. Immunbiol. 188, 36–50CrossRefGoogle Scholar
  10. 10.
    Reinhold, D., Bank, U., Bühling, F., Kähne, T., Kunz, D., Faust, J., Neubert, K., Ansorge, S. (1994) Inhibitors of Dipeptidyl peptidase IV (DPIV, CD26) specifically suppress proliferation and modulate cytokine production of strongly CD26 expressing U937 cells. Immunbiol. 192, 121–136CrossRefGoogle Scholar
  11. 11.
    Murata, M., Kubota, Y., Tanaka, T., lida-Tanaka, K., Takahara, J., Inno, S. (1994) Effect of ubenimex on the proliferation and differentation of U937 human histiocytic lymphoma cells. Leukemia 8, 2188–2193PubMedGoogle Scholar
  12. 12.
    Shibuya, K., Chiba, S., Hino, M., Kitamura, T., Miyagawa, K., Takaku, F., Miyazano, K. (1991) Enhancing effect of ubenimex (Bestatin) on proliferation and differentiation of hematopoietic progenitor cells, and the suppressive effect on proliferation of leukemic cell lines via peptidase regulation. Biomed. Pharmacother. 45, 71–80Google Scholar
  13. 13.
    Hass, R., Meinhardt, G., Hadam, M., Bartels, H. (1994) Characterization of human TUR leukemia cells: continued cell cycle progression in the presence of phorbol esters is associated with the resistance to apoptosis. Eur. J. Cell. Biol. 65, 408–416Google Scholar
  14. 14.
    Hinter, H.J. (1984) Characterization of human alanine aminopeptidases. Symposia Biologica Hungaria 25Google Scholar
  15. 15.
    Maclntyre, E.A.,Roberts, P.J., Jones, M., van der Schoot, C.E., Favalaro,E.J., Tidman, N., Linch, D.C. (1989) Activation of human monocytes occurs on cross-linking monocytic antigens to an Fc receptor. J. Immunol. 142, 2377–2383Google Scholar
  16. 16.
    Maclntyre, E.A.,Roberts, P.J.,Abdul-Gaffar, R., O’Flynn, K., Pilkington, G.R., Farace, F., Morgan, J., Linch, D.C. (1988) Mechanism of human monocytes activation via the 40-kDa Fe-receptor for IgG. J. Immunol. 141, 4333–4343Google Scholar
  17. 17.
    Hollenberg, M. D. (1996) Protease-mediated signalling: new paradigms for cell regulation and drug devcloment. TIPS 17, 3–6PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • M. Löhn
    • 1
  • C. Mueller
    • 1
  • K. Thiele
    • 1
  • T. Kähne
    • 2
  • D. Riemann
    • 1
  • J. Langner
    • 1
  1. 1.Institute of Medical ImmunologyMartin Luther University Halle/SaaleHalle/SaaleGermany
  2. 2.Institute of Experimental Internal MedicineOtto von Guericke UniversityMagdeburgGermany

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