Co-Incubation of Lymphocytes with Fibroblast-Like Synoviocytes and other Cell Types Can Induce Lymphocytic Surface Expression of Aminopeptidase N/CD13
Aminopeptidase N (APN, EC 188.8.131.52) is an ubiquitously occurring transmembrane ectoenzyme. Sequence comparisons of the cloned cDNA showed that APN is identical to CD13 antigen1. The coding part of the CD13 gene is encoded by 20 exons2. CD13 has been considered to be a myeloid-specific marker because lymphocytes of peripheral blood and tonsills always are CD13 negative. However, we have previously demonstrated the expression of CD l3 on synovial T cells from patients with different forms of arthritis3 or on tumor-infiltrating lymphocytes from renal cell carcinoma4. To learn more about conditions in tissues resulting in the expression of CD13 on lymphocytes, we incubated tonsillar T and B cells as well as peripheral blood lympho-cytes (PBL) with various mediators in the absence and presence of different stromal cells.
KeywordsCD13 mRNA Human Renal Cell Carcinoma Human Renal Cell Carcinoma Cell Kidney Tubular Epithelial Cells11 Natural Killer Cell Surface
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