Cell Culture in the Study of Epithelial Development and Function

  • F. V. Sepúlveda

Abstract

Certain cell lines derived from epithelial tissues have been demonstrated to retain in vitro some of the characteristics of their tissue of origin. Polarity of membrane proteins involved in transepithelial transport has been one of the most interesting characteristics that are preserved in these lines, thus allowing the study of ion and non-electrolyte transport in epithelia of simplified cellular composition. In addition they have become the model of choice in studies of the cell biology of membrane polarity generation.

Keywords

Apical Membrane MDCK Cell Basolateral Membrane Confluent Monolayer Transepithelial Transport 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • F. V. Sepúlveda
    • 1
  1. 1.AFRC Institute of Animal Physiology and Genetics ResearchCambridgeEngland

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