Single-Channel and Whole-Cell Patch-Clamp Experiments on Gland Cells: Activation of Ion Channels Via Internal Messengers

  • Ole Petersen


Electrophysiological studies on gland cells have contributed much to our understanding of the various membrane transport events and the stimulus-secretion coupling sequences. The earliest microelectrode studies on exocrine glands were carried out by Lundberg (1958) and later extended by Petersen (1980). The small size of mammalian gland cells made proper voltage clamp studies of transmembrane ionic currents difficult, but the first such studies with intracellular microelectrodes were made on pancreatic acini by McCandless et al. (1981) and Maruyama & Petersen (1983). At this time, however, the improved patch-clamp technique (Hamill et al. (1981) had become available and the first studies using this technique on exocrine glands were made by Maruyama and Petersen (1982a,b) on membrane fragments and intact single pancreatic acinar cells. This review aims to explain how the various patch-clamp recording configurations have been used to investigate the mechanisms by which receptor-activation switches on ion channels in exocrine gland cells.


Acinar Cell Pancreatic Acinar Cell Pancreatic Acinus Inositol Trisphosphate Mouse Pancreatic Acinar Cell 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Ole Petersen
    • 1
  1. 1.M.R.C. Secretory Control Research Group, The Physiological LaboratoryUniversity of LiverpoolLiverpoolUK

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