Chemical and Molecular Probes of Nucleoside Transport Mechanisms in Mammalian Tissues

  • Simon M. Jarvis


The passage of nucleosides and synthetic nucleoside analogues across plasma membranes of animal cells may occur by simple diffusion or more frequently by carrier-mediated processes. Two types of facilitated-diffusion nucleoside transporters that differ in their sensitivity to inhibition by nitrobenzylthioinosine (NBMPR) have been recognized (Jarvis and Young, 1987; Jarvis, 1988a; Plagemann et al., 1988). More recently, active nucleoside transport systems have been described in brush-border membrane vesicles and cells derived from renal and intestinal epithelia (Jarvis et al., 1989; Vijayalakshmi and Belt, 1988; Jakobs and Paterson, 1986). This article reviews results of recent studies of nucleoside transport in mammalian cells with particular emphasis on the methodologies employed in using chemical and molecular probes to explore the properties of nucleoside transporters.


Human Erythrocyte Purine Nucleoside Nucleoside Transport Pyrimidine Nucleoside Photoaffinity Labelling 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Simon M. Jarvis
    • 1
  1. 1.Biological LaboratoryUniversity of KentCanterbury, KentUK

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