Skip to main content

Kinetics of Transport: Characterizing the Interaction of Substrates and Inhibitors with Carrier Systems

  • Chapter

Abstract

Unlike soluble enzymes, the proteins involved in membrane transport are vectorial catalysts that operate between two compartments and as a result the kinetic study of transport requires specialized theoretical and practical approaches which do not have a counterpart in enzymology.

Keywords

  • Free Carrier
  • Exit Rate
  • High Substrate Concentration
  • Label Substrate
  • Choline Transport

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

This is a preview of subscription content, access via your institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-4757-9601-8_1
  • Chapter length: 17 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   189.00
Price excludes VAT (USA)
  • ISBN: 978-1-4757-9601-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   249.99
Price excludes VAT (USA)
Hardcover Book
USD   349.99
Price excludes VAT (USA)

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Andersen, 0.S, 1989, Kinetics of ion movement mediated by carriers and channels. Methods in Enzymology 171: 62–112.

    PubMed  CrossRef  CAS  Google Scholar 

  • Boyd, C.A.R. and Shennan, D.B. 1986, Human placental sulphate transport: studies on chorionic trophoblast brush bonier membrane vesicles. J. PhysioL (London) 377:15–24.

    Google Scholar 

  • Cabantchik, Z.I., 1983, Structure-function relations in Band 3 protein, in:“Structure and Function of Membrane Proteins”,E Quagliariello and F. Palmieri, Eds., Elsevier, Amsterdam, 271–281.

    Google Scholar 

  • Deves, R. and Krupka, R.M., 1979a, A general kinetic analysis of transport.Tests of the carrier model based on predicted relations among experimental parameters. Biochim. Biophys. Acta 556:533–547.

    Google Scholar 

  • Deves, R. and Krupka, R.M., 1979b. A simple experimental approach to the determination of carrier transport parameters for unlabelled substrate analogs. Biochem. Biophys. Acta, 556:524–532.

    Google Scholar 

  • Deves, R. and Krupka, R.M., 1981a, Evidence for a two state mobile carrier mechanism in erythrocyte choline transport: Effects of substrate analogs in inactivation of the carrier by N-ethylmaleimide. J. Membrane Biol. 61:21–30.

    Google Scholar 

  • Deves, R. and Krupka, R.M., 1981b, Reaction of internal forms of the choline carrier of erythrocytes with N-ethylmaleimide:evidence for carrier conformational change on complex formation. J. Membrane Biol., 63:99–103.

    CrossRef  CAS  Google Scholar 

  • Deves, R. and Krupka, R.M., 1983, Apparent noncompetitive inhibition of choline transport in erythrocytes by inhibitors bound at the substrate site. J. Membrane Biol. 74:183–189.

    Google Scholar 

  • Deves, R. and Krupka,R.M., 1987, Effects on transport of rapidly penetrating, competing substrate: activation and inhibition of the choline carrier of erythrocytes by imidazole. J. Membrane Biol. 99:13–23.

    CrossRef  CAS  Google Scholar 

  • Deves, R. and Boyd, C.A.R., 1989, The determination of kinetic parameters for carrier-mediated transport of non-labelled substrate analogues: a general method applied to the study of divalent anion transport in placental membrane vesicles. Proc. R. Soc. Lond. B237:85–97.

    Google Scholar 

  • Deves, R. and Krupka, R.M., 1989, Inhibition kinetics of carrier systems. Methods in Enzymology 171: 113–132.

    CrossRef  CAS  Google Scholar 

  • Deves, R. and Krupka, R.M., 1990, A simple test for the sidedness of binding of transport inhibitors, Biochim. Biophys. Acta in press.

    Google Scholar 

  • Deves, R. and Krupka, R.M., 1990, Inhibition of choline transport in erythrocytes by nalkanols. Biochim. Biophys. Acta in press.

    Google Scholar 

  • Edwards, P.A.W., 1973, Evidence for the carrier model of transport from the inhibition by N-ethylmaleimide of choline transport across the human red cell membrane. Biochim. Biophys. Acta 311:123–140.

    PubMed  CrossRef  CAS  Google Scholar 

  • Jarvis, S.M., Hammond, J.R., Paterson, A.R.P. and Clanachan, A.S.,1983, Nucleoside transport in human erythrocytes. A simple carrier with directional symmetry in fresh cells, but with directional asymmetry in cells from outdated blood. Biochem J., 210: 457–461.

    Google Scholar 

  • Krupka, R.M. and Deves, R.,1980, The choline transport system of erythrocytes. Distribution of the free carrier in the membrane. Biochim. Biophys. Acta 600: 228–232.

    PubMed  CrossRef  CAS  Google Scholar 

  • Krupka, R.M., and Deves, R., 1981, An experimental test for cyclic versus linear transport models. The mechanism of glucose and choline transport in erythrocytes. J. Biol. Chem. 256:5410–5416.

    Google Scholar 

  • Krupka, R.M. and Deves, R., 1983, Kinetics of inhibition of transport systems. Int. Rev. Cytol. 84:303–352.

    Google Scholar 

  • Krupka, R.M. and Deves, R., 1988, The choline carrier of erythrocytes: location of the NEM-reactive thiol group in the inner gated channel. J. Membrane Biol. 101:43–47.

    CrossRef  CAS  Google Scholar 

  • Hoare, D.G., 1972, The transport of L-leucine in human erythrocytes: a new kinetic analysis. J. Physiol. (London). 221:311–331.

    Google Scholar 

  • Levine, M., Oxender, D.L. and Stein, W.D., 1965, The substrate-facilitated transport of the glucose carrier across the human erythrocyte membrane. Biochem. Biophys. Acta. 109:151–163.

    Google Scholar 

  • Martin, K, 1971, Some properties of an SH group essential for choline transport in human erythrocytes. J. Physiol (London). 213:647–667.

    PubMed  CAS  Google Scholar 

  • Passow, 1987, Molecular aspects of band 3 protein-mediated anion transport across the cell membrane. Rev. Physiol. Biochem Pharmacol. 103:62–85.

    Google Scholar 

  • Stein, W.D., 1986, “Transport and Diffusion across Cell Membranes”, Academic Press, Orlando, Florida.

    Google Scholar 

  • Stein, W.D., 1989, Kinetics of transport: analyzing,testing and characterizing models using kinetic approaches. Methods in Enzymology 171: 23–61.

    CAS  Google Scholar 

  • Wilbrandt, W., and Rosenberg, T., 1961, The concept of carrier transport and its corollaries in pharmacology. Pharmacol. Rev. 13:109–183.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 1991 Springer Science+Business Media New York

About this chapter

Cite this chapter

Deves, R. (1991). Kinetics of Transport: Characterizing the Interaction of Substrates and Inhibitors with Carrier Systems. In: Yudilevich, D.L., Devés, R., Perán, S., Cabantchik, Z.I. (eds) Cell Membrane Transport. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9601-8_1

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-9601-8_1

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9603-2

  • Online ISBN: 978-1-4757-9601-8

  • eBook Packages: Springer Book Archive