Proton NMR Studies of Transmembrane Solute Transport

  • G. F. King
  • C. A. R. Boyd


Although the ability of nuclear magnetic resonance (NMR) spectroscopy to monitor chemical reactions non-invasively in intact cells and tissues has been well documented (Kuchel, 1981; Gadian, 1982; Brindle and Campbell, 1987) surprisingly few attempts have been made to review the wealth of NMR techniques available for studying transmembrane solute transport. It is the aim of this Chapter to summarise these techniques and to provide practical examples of each to guide the non-expert NMR user in the choice of appropriate NMR experiments for the transport system to be investigated. Discussion will be restricted to 1H NMR studies, since ‘H (proton) nuclei are the most NMR-responsive and are present in most biologically important molecules; those readers interested in studies employing other nuclei, such as the alkali metal ions, should consult an excellent recent review (Kirk, 1990).


Human Erythrocyte Nuclear Magnetic Resonance Spectroscopy Magnetic Field Gradient Nuclear Magnetic Resonance Technique Nuclear Magnetic Resonance Method 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • G. F. King
    • 1
  • C. A. R. Boyd
    • 2
  1. 1.Dept of BiochemistryUniv. of SydneySydneyAustralia
  2. 2.Dept of Human AnatomyUniversity of OxfordOxfordEngland

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