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Biomedical Nuclear Magnetic Resonance Spectroscopy and Transport

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Abstract

Nuclear Magnetic Resonance (NMR) spectroscopy, can be used for transport studies either directly, by elucidating metabolic pathways, transport into and out of organs and organelles, or indirectly via determination of ATP concentration, intracellular pH, viscosity, flux rates and thermodynamic information. Previously the tool of the chemist, NMR has now found many applications in biology and medicine. This advance has been made possible largely due to technological advances, allowing wider bores and stronger magnetic fields to be achieved. In this chapter I will firstly outline briefly the theory behind NMR so that its advantages and limitations can be appreciated. I will then detail, with examples, the information available from NMR Spectroscopy. This will be followed by discussion of the more biologically useful NMR nuclei. My emphasis throughout will be on in vivo applications and how NMR may be useful for transport studies. This chapter is not a comprehensive review of NMR spectroscopy nor is it written for the expert. I have erred towards being didactic and have not given references to back up every statement made, for which I apologise to all concerned. I hope it shines some light on the technique and that it may even persuade the reader to have a go!

Keywords

  • Nuclear Magnetic Resonance
  • Nuclear Magnetic Resonance Spectrum
  • Nuclear Magnetic Resonance Spectroscopy
  • Nuclear Magnetic Resonance Study
  • Nuclear Magnetic Resonance Signal

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Wray, S. (1991). Biomedical Nuclear Magnetic Resonance Spectroscopy and Transport. In: Yudilevich, D.L., Devés, R., Perán, S., Cabantchik, Z.I. (eds) Cell Membrane Transport. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9601-8_15

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  • DOI: https://doi.org/10.1007/978-1-4757-9601-8_15

  • Publisher Name: Springer, Boston, MA

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