Kinetics of Transport: Characterizing the Interaction of Substrates and Inhibitors with Carrier Systems

  • Rosa Deves


Unlike soluble enzymes, the proteins involved in membrane transport are vectorial catalysts that operate between two compartments and as a result the kinetic study of transport requires specialized theoretical and practical approaches which do not have a counterpart in enzymology.


Free Carrier Exit Rate High Substrate Concentration Label Substrate Choline Transport 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Andersen, 0.S, 1989, Kinetics of ion movement mediated by carriers and channels. Methods in Enzymology 171: 62–112.PubMedCrossRefGoogle Scholar
  2. Boyd, C.A.R. and Shennan, D.B. 1986, Human placental sulphate transport: studies on chorionic trophoblast brush bonier membrane vesicles. J. PhysioL (London) 377:15–24.Google Scholar
  3. Cabantchik, Z.I., 1983, Structure-function relations in Band 3 protein, in:“Structure and Function of Membrane Proteins”,E Quagliariello and F. Palmieri, Eds., Elsevier, Amsterdam, 271–281.Google Scholar
  4. Deves, R. and Krupka, R.M., 1979a, A general kinetic analysis of transport.Tests of the carrier model based on predicted relations among experimental parameters. Biochim. Biophys. Acta 556:533–547.Google Scholar
  5. Deves, R. and Krupka, R.M., 1979b. A simple experimental approach to the determination of carrier transport parameters for unlabelled substrate analogs. Biochem. Biophys. Acta, 556:524–532.Google Scholar
  6. Deves, R. and Krupka, R.M., 1981a, Evidence for a two state mobile carrier mechanism in erythrocyte choline transport: Effects of substrate analogs in inactivation of the carrier by N-ethylmaleimide. J. Membrane Biol. 61:21–30.Google Scholar
  7. Deves, R. and Krupka, R.M., 1981b, Reaction of internal forms of the choline carrier of erythrocytes with N-ethylmaleimide:evidence for carrier conformational change on complex formation. J. Membrane Biol., 63:99–103.CrossRefGoogle Scholar
  8. Deves, R. and Krupka, R.M., 1983, Apparent noncompetitive inhibition of choline transport in erythrocytes by inhibitors bound at the substrate site. J. Membrane Biol. 74:183–189.Google Scholar
  9. Deves, R. and Krupka,R.M., 1987, Effects on transport of rapidly penetrating, competing substrate: activation and inhibition of the choline carrier of erythrocytes by imidazole. J. Membrane Biol. 99:13–23.CrossRefGoogle Scholar
  10. Deves, R. and Boyd, C.A.R., 1989, The determination of kinetic parameters for carrier-mediated transport of non-labelled substrate analogues: a general method applied to the study of divalent anion transport in placental membrane vesicles. Proc. R. Soc. Lond. B237:85–97.Google Scholar
  11. Deves, R. and Krupka, R.M., 1989, Inhibition kinetics of carrier systems. Methods in Enzymology 171: 113–132.CrossRefGoogle Scholar
  12. Deves, R. and Krupka, R.M., 1990, A simple test for the sidedness of binding of transport inhibitors, Biochim. Biophys. Acta in press.Google Scholar
  13. Deves, R. and Krupka, R.M., 1990, Inhibition of choline transport in erythrocytes by nalkanols. Biochim. Biophys. Acta in press.Google Scholar
  14. Edwards, P.A.W., 1973, Evidence for the carrier model of transport from the inhibition by N-ethylmaleimide of choline transport across the human red cell membrane. Biochim. Biophys. Acta 311:123–140.PubMedCrossRefGoogle Scholar
  15. Jarvis, S.M., Hammond, J.R., Paterson, A.R.P. and Clanachan, A.S.,1983, Nucleoside transport in human erythrocytes. A simple carrier with directional symmetry in fresh cells, but with directional asymmetry in cells from outdated blood. Biochem J., 210: 457–461.Google Scholar
  16. Krupka, R.M. and Deves, R.,1980, The choline transport system of erythrocytes. Distribution of the free carrier in the membrane. Biochim. Biophys. Acta 600: 228–232.PubMedCrossRefGoogle Scholar
  17. Krupka, R.M., and Deves, R., 1981, An experimental test for cyclic versus linear transport models. The mechanism of glucose and choline transport in erythrocytes. J. Biol. Chem. 256:5410–5416.Google Scholar
  18. Krupka, R.M. and Deves, R., 1983, Kinetics of inhibition of transport systems. Int. Rev. Cytol. 84:303–352.Google Scholar
  19. Krupka, R.M. and Deves, R., 1988, The choline carrier of erythrocytes: location of the NEM-reactive thiol group in the inner gated channel. J. Membrane Biol. 101:43–47.CrossRefGoogle Scholar
  20. Hoare, D.G., 1972, The transport of L-leucine in human erythrocytes: a new kinetic analysis. J. Physiol. (London). 221:311–331.Google Scholar
  21. Levine, M., Oxender, D.L. and Stein, W.D., 1965, The substrate-facilitated transport of the glucose carrier across the human erythrocyte membrane. Biochem. Biophys. Acta. 109:151–163.Google Scholar
  22. Martin, K, 1971, Some properties of an SH group essential for choline transport in human erythrocytes. J. Physiol (London). 213:647–667.PubMedGoogle Scholar
  23. Passow, 1987, Molecular aspects of band 3 protein-mediated anion transport across the cell membrane. Rev. Physiol. Biochem Pharmacol. 103:62–85.Google Scholar
  24. Stein, W.D., 1986, “Transport and Diffusion across Cell Membranes”, Academic Press, Orlando, Florida.Google Scholar
  25. Stein, W.D., 1989, Kinetics of transport: analyzing,testing and characterizing models using kinetic approaches. Methods in Enzymology 171: 23–61.Google Scholar
  26. Wilbrandt, W., and Rosenberg, T., 1961, The concept of carrier transport and its corollaries in pharmacology. Pharmacol. Rev. 13:109–183.Google Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Rosa Deves
    • 1
  1. 1.Department of Physiology and Biophysics Faculty of MedicineUniversity of Chile CasillaSantiagoChile

Personalised recommendations