Abstract
Unlike soluble enzymes, the proteins involved in membrane transport are vectorial catalysts that operate between two compartments and as a result the kinetic study of transport requires specialized theoretical and practical approaches which do not have a counterpart in enzymology.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Andersen, 0.S, 1989, Kinetics of ion movement mediated by carriers and channels. Methods in Enzymology 171: 62–112.
Boyd, C.A.R. and Shennan, D.B. 1986, Human placental sulphate transport: studies on chorionic trophoblast brush bonier membrane vesicles. J. PhysioL (London) 377:15–24.
Cabantchik, Z.I., 1983, Structure-function relations in Band 3 protein, in:“Structure and Function of Membrane Proteins”,E Quagliariello and F. Palmieri, Eds., Elsevier, Amsterdam, 271–281.
Deves, R. and Krupka, R.M., 1979a, A general kinetic analysis of transport.Tests of the carrier model based on predicted relations among experimental parameters. Biochim. Biophys. Acta 556:533–547.
Deves, R. and Krupka, R.M., 1979b. A simple experimental approach to the determination of carrier transport parameters for unlabelled substrate analogs. Biochem. Biophys. Acta, 556:524–532.
Deves, R. and Krupka, R.M., 1981a, Evidence for a two state mobile carrier mechanism in erythrocyte choline transport: Effects of substrate analogs in inactivation of the carrier by N-ethylmaleimide. J. Membrane Biol. 61:21–30.
Deves, R. and Krupka, R.M., 1981b, Reaction of internal forms of the choline carrier of erythrocytes with N-ethylmaleimide:evidence for carrier conformational change on complex formation. J. Membrane Biol., 63:99–103.
Deves, R. and Krupka, R.M., 1983, Apparent noncompetitive inhibition of choline transport in erythrocytes by inhibitors bound at the substrate site. J. Membrane Biol. 74:183–189.
Deves, R. and Krupka,R.M., 1987, Effects on transport of rapidly penetrating, competing substrate: activation and inhibition of the choline carrier of erythrocytes by imidazole. J. Membrane Biol. 99:13–23.
Deves, R. and Boyd, C.A.R., 1989, The determination of kinetic parameters for carrier-mediated transport of non-labelled substrate analogues: a general method applied to the study of divalent anion transport in placental membrane vesicles. Proc. R. Soc. Lond. B237:85–97.
Deves, R. and Krupka, R.M., 1989, Inhibition kinetics of carrier systems. Methods in Enzymology 171: 113–132.
Deves, R. and Krupka, R.M., 1990, A simple test for the sidedness of binding of transport inhibitors, Biochim. Biophys. Acta in press.
Deves, R. and Krupka, R.M., 1990, Inhibition of choline transport in erythrocytes by nalkanols. Biochim. Biophys. Acta in press.
Edwards, P.A.W., 1973, Evidence for the carrier model of transport from the inhibition by N-ethylmaleimide of choline transport across the human red cell membrane. Biochim. Biophys. Acta 311:123–140.
Jarvis, S.M., Hammond, J.R., Paterson, A.R.P. and Clanachan, A.S.,1983, Nucleoside transport in human erythrocytes. A simple carrier with directional symmetry in fresh cells, but with directional asymmetry in cells from outdated blood. Biochem J., 210: 457–461.
Krupka, R.M. and Deves, R.,1980, The choline transport system of erythrocytes. Distribution of the free carrier in the membrane. Biochim. Biophys. Acta 600: 228–232.
Krupka, R.M., and Deves, R., 1981, An experimental test for cyclic versus linear transport models. The mechanism of glucose and choline transport in erythrocytes. J. Biol. Chem. 256:5410–5416.
Krupka, R.M. and Deves, R., 1983, Kinetics of inhibition of transport systems. Int. Rev. Cytol. 84:303–352.
Krupka, R.M. and Deves, R., 1988, The choline carrier of erythrocytes: location of the NEM-reactive thiol group in the inner gated channel. J. Membrane Biol. 101:43–47.
Hoare, D.G., 1972, The transport of L-leucine in human erythrocytes: a new kinetic analysis. J. Physiol. (London). 221:311–331.
Levine, M., Oxender, D.L. and Stein, W.D., 1965, The substrate-facilitated transport of the glucose carrier across the human erythrocyte membrane. Biochem. Biophys. Acta. 109:151–163.
Martin, K, 1971, Some properties of an SH group essential for choline transport in human erythrocytes. J. Physiol (London). 213:647–667.
Passow, 1987, Molecular aspects of band 3 protein-mediated anion transport across the cell membrane. Rev. Physiol. Biochem Pharmacol. 103:62–85.
Stein, W.D., 1986, “Transport and Diffusion across Cell Membranes”, Academic Press, Orlando, Florida.
Stein, W.D., 1989, Kinetics of transport: analyzing,testing and characterizing models using kinetic approaches. Methods in Enzymology 171: 23–61.
Wilbrandt, W., and Rosenberg, T., 1961, The concept of carrier transport and its corollaries in pharmacology. Pharmacol. Rev. 13:109–183.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Deves, R. (1991). Kinetics of Transport: Characterizing the Interaction of Substrates and Inhibitors with Carrier Systems. In: Yudilevich, D.L., Devés, R., Perán, S., Cabantchik, Z.I. (eds) Cell Membrane Transport. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9601-8_1
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9601-8_1
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9603-2
Online ISBN: 978-1-4757-9601-8
eBook Packages: Springer Book Archive