Membrane-Membrane Interactions via Intermediates in Lamellar-to-Inverted Hexagonal Phase Transitions
Recent theoretical work (Siegel, 1984, 1986a-c)shows that vesicles whose lipids can undergo the Lα/HII(lamellar-to-inverted hexagonal) phase transition should be especially susceptible to three membrane-membrane interactions: fusion, aggregation-induced vesicle leakage, and intervesicular lipid exchange. This chapter describes this model as well as experimental results derived from relevant systems. The model shows that in HII-forming systems the relative rates of the three membrane-membrane interactions may be determined by the lipid-phase behavior and the dynamics of the Lα/HIIphase transition, i.e., the rate at which A. given lipid system can execute the Lα/HIIphase transition under given conditions.
KeywordsMembrane Fusion Lipidic Particle Lipid System Aqueous Content Outer Monolayer
Unable to display preview. Download preview PDF.
- Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., and Watson, J. D., 1983, Molecular Biology of the Cell, Garland, New York.Google Scholar
- Bally, M. B., Tilcock, C. P. S., Hope, M. J., and Cullis, P. R., 1983, Polymorphism of phosphatidylethanolamine-phosphatidylserine model systems: Influence of cholesterol and Mg2+on Ca2+-triggered bilayer to hexagonal (HII) transitions, Can. J. Biochem. Cell Biol 61:346–352.PubMedCrossRefGoogle Scholar
- Bentz, J., Ellens, H., Szoka, F. C., Oliver, J., and Siegel, D., 1986, Membrane fusion, isotropic intermediates and the La/HIIphase transition, Biophys. J.49:17a.Google Scholar
- Das, S., Rand, R. P., 1985, Diacylglycerol, A. product of phosphatidylinositol metabolism, causes major structural perturbations in lipid bilayers, Biophys. J. 41:41a.Google Scholar
- Düzgünec, N., and Papahadjopoulos, D., 1983, Ionotropic effects on phospholipid membranes: Calcium/magnesium specificity in binding, fluidity, and fusion, Membrane Fluid. Biol 2:187–216.Google Scholar
- Gounaris, K., Mannock, D. A., Sen, A., Brain, A. P. R., Williams, W. P., and Quinn, P. J., 1983c, Polyunsaturated fatty acyl residues of galactolipids are involved in the control of bilayer/non-bilayer lipid transitions higher plant chloroplasts, Biochim. Biophys. Acta 732:229–242.CrossRefGoogle Scholar
- Gutman, H., Arvidson, G., Fontell, K., and Lindblom, G., 1984,31P-NMR and 2H-NMR studies of phase equilibria in the three component system monoolein-dioleoylphos-phatidylcholine-water, in: Surfactants in Solution, Vol. 1 (K. L. Mittal and B. Lindman, eds.), pp. 143–152, Plenum Press, New York.Google Scholar
- Hong, K., Baldwin, P. A., Allen, T. M., and Papahadjopoulos, D., 1986, Fluorometric detection of bilayer to hexagonal phase transitions in liposomes, Biochim. Biophys. Acta (submitted).Google Scholar
- Siegel, D. P., 1986c, Inverted micellar intermediates and the transitions between lamellar, cubic, and inverted hexagonal lipid phases. III. Isotropic and inverted cubic state formation via intermediates in transitions between Laand HIIphases, Chem. Phys. Lipids(in press).Google Scholar