Abstract
Plant cells frequently have been used for cell-fusion experiments as A. result of the development of A. technique for enzymatic digestion of the cell wall (Cocking, 1960). With this technique, A. variety of new genetic manipulations have become possible, such as protoplast fusion, uptake of organelles, and uptake of DNA. With the enzymatic digestion procedure, large populations of genetically and physiologically homogeneous protoplasts are now available (Chaleff, 1981).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bold, H. C., and Wynne, M. X., 1978, Introduction to the Algae, Prentice-Hall, Englewood Cliffs, New Jersey.
Burr, F. A., and West, J. A., 1970, Light and electron microscope observations on the vegetative and reproductive structures of Bryopsis hypnoides, Phycologia 9:17–37.
Burr, F. A., and West, J. A., 1971a, Comparative ultrastructure of the primary nucleus in Bryopsis and Acetabularia, J. Phycol 7:108–113.
Burr, F. A., and West, J. A., 1971b, Protein bodies in Bryopsis hypnoides: Their relationship to wound healing and branch septum development, J. Ultrastruct. Res. 35:476–498.
Chaleff, R. S., 1981, Genetics of higher plants—Application of cell culture, Cambridge University Press, New York, pp. 96–147.
Cocking, E. C., 1960, A. method for the isolation of plant protoplast and vacuoles, Nature (Lond.) 187:962–963.
Enomoto, S., and Hirose, H., 1972, Culture studies on artificially induced aplanospores and their development in the marine alga Boergesenia forbesii(Harvey) Feldmann (Chloro-phyceae, Siphonocladales), Phycologia 11:119–122.
Evans, D. A., Wetter, L. R., and Gamborg, O. L., 1980, Somatic hybrid plants of Nicotiana glaucaand Nicotiana tabacumobtained by protoplast fusion, Physiol. Plant.48:225–230.
Gardner, K. H., and Blackwell, J., 1971, The substructure of the cellulose microfibrils from the cell walls of the algae Valonia ventricosa, J. Ultrastruct. Res.36:725–731.
Haigler, C. H., Brown, R. M., Jr., and Benziman, M., 1980, Calcofluor white ST alters the in vivoassembly of cellulose microfibrils, Science 210:903–906.
Hain, R., Steinbiss, H. H., and Schell, J., 1984, Fusion of Agrobacteriumand E. colispheroplasts with Nicotiana tabacumprotoplasts: Direct gene transfer from microorganism to higher plant, Plant Cell Res.3:60–64.
Itoh, T., and Brown, R. M., Jr., 1984, The assembly of cellulose microfibrils in Valonia mac-rophysaKütz, Planta 160:372–381.
Itoh, T., O’Neil, R., and Brown, R. M., Jr., 1985, Interference of cell wall regeneration of Boergesenia forbesii protoplasts by Tinopal LPW, A. fluorescent brightening agent, Protoplasma 123:174–183.
Itoh, T., Legge, R. L., and Brown, R. M., Jr., 1986, The effects of selected inhibitors on cellulose microfibril assembly in Boergesenia forbesii(Chlorophyta) protoplasts, J. Phycol 22:224–233.
Kao, K. N., and Michayluk, M. R., 1974, A. method for high-frequency intergeneric fusion of plant protoplasts, Planta 115:355–367.
La Claire, J. W., II, 1982, Cytomorphological aspects of wound healing in selected si-phonocladales (Chlorophyceae), J. Phycol.18:379–384.
Preston, R. D., 1968, Plants without cellulose, Sci. Am.218:102–108.
Preston, R. D., and Kuyper, B., 1951, Electron microscopic investigations of the walls of green algae. I. A. preliminary account of wall lamellation and deposition in Valonia ventricosa, J. Exp. Bot.2:247–256.
Provasoli, L., McLaughlin, J. J. A., and Droop, M. R., 1957, The development of artificial media for marine algae, Arch. Mikrobiol.25:392–428.
Salhani, N., Vienken, J., Zimmermann, U., Ward, M., Davey, M. R., Clothier, R. H., Balls, M., Cocking, E. C., and Lucy, J. A., 1985, Haemoglobin synthesis and cell wall regeneration by electric field-induced interkingdom heterokaryons, Protoplasma 126:30–36.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer Science+Business Media New York
About this chapter
Cite this chapter
Lee, S.Y., Brown, R.M. (1987). Experimental Cell Fusion with Selected Siphonocladalean Algal Cells. In: Sowers, A.E. (eds) Cell Fusion. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9598-1_8
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9598-1_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9600-1
Online ISBN: 978-1-4757-9598-1
eBook Packages: Springer Book Archive