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Electrofusion and Plant Somatic Hybridization

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Cell Fusion

Abstract

The transfer of genetic information between sexually incompatible species creates unique experimental opportunities for geneticists and cell biologists. This is especially true of plant somatic cell fusion because the totipotency of plant cells makes it possible to grow fusion products into mature plants (reviewed by Gleba and Sytnik, 1984; Vasil and Vasil, 1980). Although Carlson et al. (1972) reported recovering the first interspecific plant hybrid through protoplast fusion in 1972, subsequent progress in this field has been slower than originally hoped. This problem is due to several biological and technical barriers. This chapter describes our progress in the application of electrofusion to plant somatic hybridization (see Bates et al., 1983; Bates, 1985; Bates and Hasenkampf, 1985) and suggests solutions to some of the problems in this field.

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References

  • Aviv, D., and Galun, E., 1984, The feeder layer technique, in: Cell Culture and Somatic Cell Genetics of Plants, Vol. 1 (I. K. Vasil, ed.), pp. 199–203, Academic Press, New York.

    Google Scholar 

  • Bates, G. W., 1985, Electrical fusion for optimal formation of protoplast heterokaryons in Nicotiana, Planta 165:217–224.

    Article  Google Scholar 

  • Bates, G. W., and Hasenkampf, C. A., 1985, Culture of plant somatic hybrids following electrical fusion, Theor. Appl. Genet.70:277–233.

    Article  Google Scholar 

  • Bates, G. W., Gaynor, J. J., and Shekhawat, N. S., 1983, Fusion of plant protoplasts by electric fusion, Plant Physiol 72:1110–1113.

    Article  PubMed  CAS  Google Scholar 

  • Brewbaker, J. L., Upadhya, M. D., Makinen, Y., and MacDonald, T., 1968, Isoenzyme polymorphism in flowering plants. 3. Gel electrophoretic methods and applications, Physiol Plant 121: 930–940.

    Article  Google Scholar 

  • Carlson, P. S., Smith, H. H., and Dearing, R. D., 1972, Parasexual interspecific plant hybridization, Proc. Natl Acad. Sci. U.S.A.69:2292–2294.

    Article  PubMed  CAS  Google Scholar 

  • Chapel, M., Montane, M. H., Ranty, B., Teissie, J., and Alibert, G., 1986, Viable somatic hybrids obtained by direct current electrofusion of chemically aggregated plant protoplasts, FEBS Lett.196:79–86.

    Article  CAS  Google Scholar 

  • Constabel, F., Dudits, D., Gamborg, O. L., and Kao, K. N., 1975a, Nuclear fusion in intergeneric heterokaryons, a note, Can. J. Bot.53:2092–2095.

    Article  Google Scholar 

  • Constabel, F., Kirkpatrick, J. W., Kao, K. N., and Kartha, K. K., 1975b, The effect of canavanine on the growth of cells from suspension cultures and on intergeneric heterokaryocytes of canavanine sensitive and tolerant plants, Biochem. Physiol. Pflanz.168:319–325.

    CAS  Google Scholar 

  • Davey, M. R., and Kumar, A., 1983, Higher plant protoplasts—retrospect and prospect, in: Plant Protoplasts, International Review of Cytology, Supplement 16 (K. L. Giles, ed.), pp. 219–299, Academic Press, New York.

    Google Scholar 

  • Evans, D. A., and Bravo, J. E., 1983, Plant protoplast isolation and culture, in: Plant Protoplasts, International Review of Cytology, Supplement 16 (K. L. Giles, ed.), pp. 33–53, Academic Press, New York.

    Google Scholar 

  • Flores, H. E., Kaur-Sawhney, R., and Galston, A. W., 1981, Protoplasts as vehicles for plant propagation and improvement, Adv. Cell Culture 1:241–279.

    CAS  Google Scholar 

  • Fluhr, R., Aviv, D., Galun, E., and Edelman, M., 1984, Generation of heteroplastidic Nicotiana cybrids by protoplast fusion: Analysis for plastid recombinant types, Theor. Appl. Genet.67:491–497.

    Article  CAS  Google Scholar 

  • Fraley, R. T., Rogers, S. G., Horsch, R. B., Sanders, P. R., Flick, J. S., Adams, S. P., Bittner, M. L., Brand, L. A., Fink, C. L., Fry, J. S., Galluppi, G. R., Goldberg, S. B., Hoffmann, N. L., and Woo, S. C., 1983, Expression of bacterial genes in plant cells, Proc. Natl. Acad. Sci. U.S.A.80:4803–4807.

    Article  PubMed  CAS  Google Scholar 

  • Fromm, M., Taylor, L. P., and Walbot, V., 1985, Expression of genes electroporated into mon-ocot and dicot plant cells, Proc. Natl. Acad. Sci. U.S.A.82:5824–5828.

    Article  PubMed  CAS  Google Scholar 

  • Gleba, Y. y, and Sytnik, K. M., 1984, Protoplast Fusion, Springer-Verlag, New York.

    Book  Google Scholar 

  • Glimelius, K., Eriksson, T., Grafe, R., and Muller, A. J., 1978, Somatic hybridization of nitrate reductase deficient mutants of Nicotiana tabacum by protoplast fusion, Physiol. Plant.44:273–277.

    Article  CAS  Google Scholar 

  • Horsch, R. B., and Jones, G. E., 1980, A double filter paper technique for plating cultured plant cells, In Vitro 16: 103–108.

    Article  Google Scholar 

  • Horsch, R. B., Fraley, R. T., Rogers, S. G., Sanders, P. R, Lloyd, A., and Hoffmann, N., 1984, Inheritance of functional foreign genes in plants, Science 223:496–498.

    Article  PubMed  CAS  Google Scholar 

  • Horsch, R. B., Fry, J. E., Hoffmann, N. L., Eichholtz, D., Rogers, S. G., and Fraley, R. T., 1985, A simple and general method for transferring genes into plants, Science 227:1129–1231.

    Google Scholar 

  • Kao, K. N., 1975a, A nuclear staining method for plant protoplasts, in: Plant Tissue Culture Methods (O. L. Gamborg and L. R. Wetter, eds.), pp. 60–62, Prairie Research Laboratory, Saskatoon.

    Google Scholar 

  • Kao, K. N., 1975b, A method for fusion of plant protoplasts with polyethylene glycol, in: Plant Tissue Culture Methods (O. L. Gamborg and L. R. Wetter, eds.), pp. 22–27, Prairie Research Laboratory, Saskatoon.

    Google Scholar 

  • Kao, K. N., 1977, Chromosomal behavior in somatic hybrids of soybean—Nicotiana glauca, Mol. Gen. Genet.150:225–230.

    Article  Google Scholar 

  • Kao, K. N., 1981, Plant protoplast fusion and somatic hybrids, in: Plant Tissue Culture (H. Han, ed.), pp. 331–339, Proceedings of the Beijing Symposium, Pitman, London.

    Google Scholar 

  • Kao, K. N., and Michayluk, M. R., 1974, A method for high frequency intergeneric fusion of plant protoplasts, Planta 115: 355–367.

    Article  CAS  Google Scholar 

  • Kartha, K. K., Gamborg, O. L., Constabel, F., and Kao, K. N., 1974, Fusion of rapeseed and soybean protoplasts and subsequent division of heterokaryocytes, Can. J. Bot.52:2435–2436.

    Article  Google Scholar 

  • Kohn, H., Schieder, R., and Schieder, O., 1985, Somatic hybrids in tobacco mediated by electrofusion, Plant Sci.38:121–128.

    Article  Google Scholar 

  • Koop, H.-U., 1985, Regeneration of plants after electrofusion of selected pairs of protoplasts, Eur. J. Cell Biol.39:46–49.

    Google Scholar 

  • Maliga, P., Lazar, G., Joo, F., Nagy, A H., and Menczel, L., 1977, Restoration of morpho-genetic potential in Nicotiana by somatic hybridization, Mol. Gen. Genet.157:291–296.

    Article  CAS  Google Scholar 

  • Menczel, L., Galiba, G., Nagy, F., and Maliga, P., 1982, Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana, Genetics 100:487–495.

    CAS  Google Scholar 

  • Miller, R. A., Gamborg, O. L., Keller, W. A., and Kao, K.N., 1971, Fusion and division of nuclei in multinculeated soybean protoplasts, Can. J. Genet. Cytol.13:347–353.

    Google Scholar 

  • Morikawa, H., Sugino, K., Hayashi, Y., Takeda, J., Senda, M., Hirai, A., and Yamada, Y., 1986, Interspecific plant hybridization by electrofusion in Nicotiana, Biotechnology 4: 57–60.

    Article  Google Scholar 

  • Nagy, J. I., and Maliga, P., 1976, Callus induction and plant regeneration from protoplasts of Nicotiana sylvestris, Z. Pflanzenphysiol.78:453–455.

    Google Scholar 

  • Negrutiu, I., DeBrouwer, D., Watts, J. W., Sidorov, V. I., Dirks, R., and Jacobs, M., 1986, Fusion of plant protoplasts: a study using auxotrophic mutants of Nicotiana plumbaginifolia Viviani, Theor. Appl. Genet.72:279–286.

    Article  Google Scholar 

  • Pohl, H. A., 1978, Dielectrophoresis, Cambridge University Press, Cambridge.

    Google Scholar 

  • Potrykus, I., Paszkowsi, J., Saul, M. W., Petruska, J., and Shillito, R. D., 1985a, Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer, Mol. Gen. Genet.199:169–177.

    Article  PubMed  CAS  Google Scholar 

  • Potrykus, I., Saul, M. W., Petruska, J., Paszkowski, J., and Shillito, R. D., 1986, Direct gene transfer to cells of a graminaceous monocot, Mol. Gen. Genet.199:183–188.

    Article  Google Scholar 

  • Power, J. B., Berry, S. F., Frearson, E. M., and Cocking, E. C., 1977, Selection procedures for the production of inter-species somatic hybrids of Petunia hybrida and Petunia parodii, Plant Sci. Lett.7:51–55.

    Google Scholar 

  • Puite, K. J., van Wikselaar, P., and Verhoeven, H., 1985, Electrofusion, a simple and reproducible technique in somatic hybridization of Nicotiana plumbaginifolia mutants, Plant Cell Rep.4:274–276.

    Article  Google Scholar 

  • Scheurich, P., and Zimmermann, U., 1981, Giant human erythrocytes by electric-field-induced cell-to-cell fusion, Naturwissenschaften 68: 45–47.

    Article  PubMed  CAS  Google Scholar 

  • Shillito, R. D., Paszkowski, J., and Potrykus, I., 1983, Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species, Plant Cell Rep.2:244–247.

    Article  CAS  Google Scholar 

  • Sidorov, V. A., Menczel, L., Nagy, F., and Maliga, P., 1981, Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts, Planta 152: 341–345.

    Article  CAS  Google Scholar 

  • Tempelaar, M. J., and Jones, M. G. K., 1985a, Directed electrofusion between protoplasts with different responses in a mass fusion system, Plant Cell Rep.4:92–95.

    Article  Google Scholar 

  • Tempelaar, M. J., and Jones, M. G. K., 1985b, Fusion characteristics of plant protoplasts in electric fields, Planta 165: 205–216.

    Article  Google Scholar 

  • Vasil, I. K., and Vasil, V., 1980, Isolation and culture of protoplasts, Int. Rev. Cytol. (Suppl.) 11A: 1–20.

    Article  Google Scholar 

  • Vienken, J., and Zimmermann, U., 1982, Electric field-induced fusion: Electro-hydraulic procedure for production of heterokaryon cells in high yield, FEBS Lett.137:11–13.

    Article  PubMed  CAS  Google Scholar 

  • Watts, J. W., and King, J. M., 1984, A simple method for large-scale electrofusion and culture of plant protoplasts, Biosci. Rep.4:335–342.

    Article  PubMed  CAS  Google Scholar 

  • Zimmermann, U., 1982, Electric field-mediated fusion and related electrical phenomena, Biochim. Biophys. Acta 694:227–277.

    Article  PubMed  CAS  Google Scholar 

  • Zimmermann, U., and Scheurich, P., 1981, High frequency fusion of plant protoplasts by electric fields, Planta 151: 26–32.

    Article  Google Scholar 

  • Zimmermann, U., Buchner, K. H., and Arnold, W. M., 1984, Electrofusion of cells: recent developments and relevance for evolution, in: Charge and Field Effects in Biosystems (M. J. Allen and P. N. R. Usherwood, eds.), pp. 293–318, Abacus Press, Normal, Ill.

    Google Scholar 

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Bates, G.W., Nea, L.J., Hasenkampf, C.A. (1987). Electrofusion and Plant Somatic Hybridization. In: Sowers, A.E. (eds) Cell Fusion. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9598-1_24

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  • DOI: https://doi.org/10.1007/978-1-4757-9598-1_24

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9600-1

  • Online ISBN: 978-1-4757-9598-1

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