Electrofusion is a new method of inducing membrane fusion that uses electric field pulses. A general review of the method will be found elsewhere in this volume (Chapter 17). This chapter reviews what is known about the mechanism of electrofusion and will include a discussion of the results from our laboratory on studies using erythrocyte ghosts as a model membrane.
KeywordsElectric Field Pulse Lucifer Yellow Pulse Treatment Erythrocyte Ghost Membrane Contact
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- Arnold, W. M., and Zimmermann, U., 1984, Electric field-induced fusion and rotation of cells, Biol Membranes 5:389–454.Google Scholar
- Chernomordik, L. V., Sukharev, S. I., and Abidor, I. G., 1985, Long-living defects in BLM after reversibly electrical breakdown, Biol Membrane 2:87–94.Google Scholar
- Pohl, H. A., 1978, Dielectrophoresis, Cambridge University Press, London.Google Scholar
- Sowers, A. E., 1983b, Red cell and red cell ghost membrane shape changes accompanying the application of electric fields for inducing fusion, J. Cell Biol. 97:179a.Google Scholar
- Sowers, A. E., 1985a, Mechanism of membrane electrofusion in erythrocyte ghosts, J. Cell Biol. 101:309a.Google Scholar
- Stenger, D. A., and Hui, S. W., 1986, Kinetics of ultrastructural changes during electrically-induced fusion of human erythrocytes, J. Membr. Biol., in press.Google Scholar
- Zimmermann, U., Vienken, J., and Pilwat, G., 1984, Electrofusion of cells, in: Investigative Microtechniques in Medicine and Biology (J. Chayen and L. Bitensky, eds.), pp. 89–167, Dekker, New York.Google Scholar
- Zimmermann, U., Vienken, J., Halfman, J., and Emois, C. C., 1985, Electrofusion: A novel hybridization technique, in: Advances in Biotechnological Processes, Vol. 4 (A Mizrahi and A. L. van Wezel, eds.), pp. 79–150, Liss, New York.Google Scholar