Abstract
Membranes provide the barrier of cellular individuality or intracellular compartmentation. Individuality or compartmentation is unlikely to be traversed except in A. well-regulated biological process, membrane fusion. To understand the fundamental mechanism of membrane fusion, simple membrane systems have been studied extensively (reviewed by Papahadjopoulos et al, 1979; Nir et al, 1983; Düzgünes, 1985). Information obtained from model membranes has been useful not only for defining the role of fusion triggers or modulators, but also for determining the fusion susceptibility of individual components of membranes. However, fusion requirements for these simple systems are often far from those known to be required for biological fusion. For example, fusion between isolated secretory vesicles occurs at much lower Ca2+concentrations than those required for fusion of liposomes made from the extracted lipids of the secretory vesicles (Gratzl et al, 1980; Ekerdt et al, 1981). A. simple explanation for this discrepancy in Ca2+threshold is that other factors are involved in the sensitivity for Ca2+in the fusion event. Among these factors, protein is considered A. likely candidate. Therefore, protein-facilitated fusion of liposomes is an important step toward the reconstitution of natural membrane fusion.
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References
Baker, P. F., and Knight, D. E., 1984, Calcium control of exocytosis in bovine adrenal medullary cells, Trends Neurosci. 7:120–126.
Barondes, S. H., 1981, Lectins: Their multiple endogenous cellular functions, Annu. Rev. Biochem. 50:207–231.
Blumenthal, R., Henkart, M., and Steer, C. J., 1983, Clathrin-induced pH-dependent fusion of phosphatidylcholine vesicles, J. Biol. Chem. 258:3409–3415.
Creutz, C. E., 1981, cis-Unsaturated fatty acids induce the fusion of chromaffin granules aggregated by synexin, J. Cell Biol. 91:247–256.
Creutz, C. E., Pazoles, C. J., and Pollard, H. B., 1978, Identification and purification of an adrenal medullary protein (synexin) that causes calcium-dependent aggregation of isolated chromaffin granules,J. Biol Chem. 253:2858–2866.
Creutz, C. E., Pazoles, C. J., and Pollard, H. B., 1979, Self-association of synexin in the presence of calcium: Correlation with synexin-induced membrane fusion and examination of the structure of synexin aggregates. J Biol. Chem. 254:553–558.
Creutz, C. E., Dowling, L. G., Sando, J. J., Villar-Palasi, C., Whipple, J. H., and Zaks, W. J., 1983, Characterization of the chromobindins: Soluble proteins that bind to the chromaffin granule membrane in the presence of Ca ,J. Biol Chem. 258:14664–14674.
Cullis, P. R., and deKruijff, B., 1978, The polymorphic phase behavior of phosphatidyl-ethanolamines of natural and synthetic origin. A. P-NMR study, Biochim. Biophys. Acta 513:31–42.
Dan, J. C., 1967, Acrosome reaction and lysins, in: Fertilization, Vol. 1 (C. B. Metz and A. Monroy, eds.), pp. 237–293, Academic Press, New York.
Dunn, L. A., and Holz, R. W., 1983, Catecholamine secretion from digitonin-treated adrenal medullary chromaffin cells,J. Biol Chem. 258:4989–4993.
Düzgünes, N., 1985, Membrane fusion, in: Subcellular Biochemistry, Vol. 11, (D. B. Roodyn, ed.), pp. 195–286, Plenum Press, New York.
Düzgünes, N., Wilschut, J., Fraley, R., and Papahadjopoulos, D., 1981, Studies on the mechanism of membrane fusion: Role of head-group composition in calcium- and magnesium-induced fusion of mixed phospholipid vesicles, Biochim. Biophys. Acta 642:182–195.
Düzgünes, N., Hoekstra, D., Hong, K., and Papahadjopoulos, D., 1984, Lectins facilitate calcium-induced fusion of phospholipid vesicles containing glycosphingolipids, FEBS Lett. 173:80–84.
Düzgünes, N., Wilschut, J., and Papahadjopoulos, D., 1985, Control of membrane fusion by divalent cations, phospholipid head-groups and proteins, in: Physical Methods on Biological Membranes and Their Model Systems (F. Conti, W. E. Blumberg, J. de Gier, and F. Pocchiari, eds.), pp. 193–218, Plenum Press, New York.
Ekerdt, R., Dahl, G., and Gratzl, M., 1981, Membrane fusion of secretory vesicles and liposomes. Two different types of fusion, Biochim. Biophys. Acta 646:10–22.
Ellens, H., Bentz, J. and Szoka, F. C., 1985, H+- and Ca 2+-induced fusion and destabilization of liposomes, Biochemistry 24:3099–3106.
Ernst, J. D., Meers, P., Düzgünes, N., Hong, K., Scannell, K., Papahadjopoulos, D., and Goldstein, I. M., 1985, A. synexin-like protein in the cytosol of polymorphonuclear leukocytes mediates calcium-dependent granule aggregation, Clin. Res.33:401a.
Fridberger, A., Sundelin, J., Vacquier, V. D., and Peterson, P. A., 1985, Amino acid sequence of an egg-lysin protein from abalone spermatozoa that solubilizes the vitelline layer, J. Biol. Chem. 260:9092–9099,
Glabe, C. G., 1985a, Interaction of the sperm adhesive protein, bindin, with phospholipid vesicles, I. Specific association of bindin with gel-phase phospholipid vesicles, J. Cell Biol. 100:794–799.
Glabe, C. G., 1985b, Interaction of the sperm adhesive protein, bindin, with phospholipid vesicles, II. Bindin induces the fusion of mixed-phase vesicles that contain phosphatidylcholine and phosphatidylserine in vitro, J. Cell Biol. 100:800–806.
Glabe, C. G., and Lennarz, W. J., 1979, Species-specific sperm adhesion in sea urchins: A. quantitative investigation of bindin-mediated egg agglutination,J. Cell Biol. 83:595–604.
Glabe, C. G., and Vacquier, V. D., 1977, Species specific agglutination of eggs by bindin isolated from sea urchin sperm, Nature (Lond.) 267:836–837.
Glabe, C. G., Grabel, L. B., Vacquier, V. D., and Rosen, S. D., 1982, Carbohydrate specificity of sea urchin sperm bindin: A. cell surface lectin mediating sperm-egg adhesion,J. Cell Biol. 94:123–128.
Gratzl, M., Schudt, C., Ekerdt, R., and Dahl, G., 1980, Fusion of isolated biological membranes: A. tool to investigate basic processes of exocytosis and cell-cell fusion, in: Membrane Structure and Function, Vol. 3 (E. E. Bittar, ed.), pp. 59–92, Wiley, New York.
Hoekstra, D., and Düzgünes, N., 1986, Ricinus communis agglutinin-mediated agglutination and fusion of glycolipid-containing phospholipid vesicles, Effect of carbohydrate headgroup size, calcium ions, and spermine, Biochemistry 25:1321–1330.
Hoekstra, D., Düzgünes, N., and Wilschut, J., 1985, Agglutination and fusion of globoside GL-4 containing phospholipid vesicles mediated by lectins and calcium ions, Biochemistry 24:565–572.
Hong, K., and Vacquier, V. D., 1986, Fusion of liposomes induced by A. cationic protein from the acrosome granule of abalone spermatozoa, Biochemistry, 25:543–549.
Hong, K., Düzgünes, N., and Papahadjopoulos, D., 1981, Role of synexin in membrane fusion, J. Biol. Chem. 256:3641–3644.
Hong, K., Düzgünes, N., and Papahadjopoulos, D., 1982a, Modulation of membrane fusion by calcium-binding proteins, Biophys. J. 37:297–305.
Hong, K., Düzgünes, N., Ekerdt, R., and Papahadjopoulos, D., 1982b, Synexin facilitates fusion of specific phospholipid vesicles at divalent cation concentrations found in-tracellularly, Proc. Natl. Acad. Sci. USA. 79:4942–4944.
Hong, K., Ekerdt, R., Bentz, J., Nir, S., and Papahadjopoulos, D., 1983, Kinetics of synexin-facilitated membrane fusion, Biophys. J., 41:31a.
Hong, K., Yoshimura, T., and Papahadjopoulos, D., 1985, Interaction of clathrin with liposomes: pH-dependent fusion of phospholipid membranes induced by clathrin, FEBS Lett 191:17–23.
Irvine, R. F., 1982, How is the level of free arachidonic acid controlled in mammalian cells?, Biochem.J. 204:3–16.
Jendrasiak, G. L., and Hasty, J. H., 1974, The hydration of phospholipids, Biochim. Biophys. Acta 337:79–91.
Lewis, C. A., Talbot, C. F., and Vacquier, V. D., 1982, A. protein from abalone sperm dissolves the egg vitelline layer by A. nonenzymatic mechanism, Dev. Biol. 92:227–239.
Meers, P., Ernst, J., Hong, K., Düzgünes, N., Goldstein, I. M., and Papahadjopoulos, D., 1986, Synexin-like proteins from the cytosol of human neutrophils mediate aggregation and apparent fusion of specific granules and liposomes, Biophys. J. 49:134a.
Morris, S. J., Hughes, J. M. X., and Whittaker, V. P., 1982, Purification and mode of action of synexin: A. protein enhancing calcium-induced membrane aggregation,J. Neurochem. 39:529–536.
Nir, S., Bentz, J., and Wilschut, J., 1980, Mass action kinetics of phosphatidylserine vesicle fusion as monitored by coalescence of internal vesicle volumes, Biochemistry 19:6030–6036.
Nir, S., Bentz, J., Wilschut, J., and Düzgünes, 1983, Aggregation and fusion of phospholipid vesicles, Prog. Surface. Sci. 13:1–124.
Papahadjopoulos, D., Poste, G., and Vail, W. J., 1979, Studies on membrane fusion with natural and model membranes, Methods Membrane Biol. 10:1–121.
Pollard, H. B., and Scott, J. H., 1982, Synhibin: A. new calcium-dependent membrane-binding protein that inhibits synexin-induced chromaffin granule aggregation and fusion, FEBS Lett. 150:201–206.
Reiss-Husson, F., 1967, Structure des phases liquide-cristallines de différents phospho-lipides, monoglycérides, sphingolipides, anhydres ou en présence d’eau, J. Molec. Biol. 25:363–382.
Rosenberg, J., Düzgünes, N., and Kayalar, C., 1983, Comparison of two liposome fusion assays monitoring the intermixing of aqueous contents and of membrane components, Biochim. Biophys. Acta 735:173–180.
Struck, D. K., Hoekstra, D., and Pagano, R. E., 1981, Use of resonance energy transfer to monitor membrane fusion, Biochemistry 20:4093–4099.
Südhof, T. C., Walker, J. H., and Obrocki, J., 1982, Calelectrin self-aggregates and promotes membrane aggregation in the presence of calcium, EMBO J. 1:1167–1170.
Südhof, T. C., Ebbecke, M., Walker, J. H., Fritsche, U., and Boustead, C., 1984, Isolation of mammalian calelectrins; A. new class of ubiquitous Ca 2+-regulated proteins, Biochemistry 23:1103–1109.
Sundler, R., and Papahadjopoulos, D., 1981, Control of membrane fusion by phospholipid head-groups I. Phosphatidate/phosphatidylinositol specificity, Biochim. Biophys. Acta 649:743–750.
Sundler, R., and Wijkander, J., 1983, Protein-mediated intermembrane contact specifically enhances Ca 2+-induced fusion of phosphatidate-containing membranes, Biochim. Biophys. Acta. 730:391–394.
Vacquier, V. D., and Moy, G. W., 1977, Isolation of bindin: The protein responsible for adhesion of sperm to sea urchin eggs, Proc. Natl. Acad. Sci. USA. 74:2456–2460.
Wilschut, J., and Papahadjopoulos, D., 1979, Ca2+-induced fusion of phospholipid vesicles monitored by mixing of aqueous contents, Nature (Lond.) 281:690–692.
Wilschut, J., Düzgünes, N., Fraley, R., and Papahadjopoulos, D., 1980, Studies on the mechanism of membrane fusion: Kinetics of calcium ion induced fusion of phosphatidylserine vesicles followed by A. new assay for mixing of aqueous vesicle contents, Biochemistry 19:6011–6021.
Wilschut, J., Düzgünes, N., and Papahadjopoulos, D., 1981, Calcium/magnesium specificity in membrane fusion: Kinetics of aggregation and fusion of phosphatidylserine vesicles and the role of bilayer curvature, Biochemistry 20:3126–3133.
Wilson, S. P., and Kirshner, N., 1983, Calcium-evoked secretion from digitonin-permeabilized adrenal medullary chromaffin cells, J. Biol. Chem. 258:4994–5000.
Wolf, D. E., Kinsey, W. H., Lennarz, W. J., and Edidin, M., 1981, Changes in the organization of the sea urchin plasma membrane upon fertilization: Indications from the lateral diffusion rates of lipid-soluble fluorescent dyes, Dev. Biol. 81:133–138.
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Hong, K., Düzgüneş, N., Meers, P.R., Papahadjopoulos, D. (1987). Protein Modulation of Liposome Fusion. In: Sowers, A.E. (eds) Cell Fusion. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9598-1_12
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DOI: https://doi.org/10.1007/978-1-4757-9598-1_12
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