Subcellular Localization of a Boronated Nucleoside Using Ion Microscopy
The success of boron neutron capture therapy (BNCT) is dependent on the uptake and retention of a sufficient quantity of boron by tumor cells. It has been calculated that the concentration of 10B delivered to the tumor must be in the range of 5–30 μg/g 1. As more boronated compounds are developed for possible use in BNCT, it becomes increasingly more important to have quick effective techniques for screening the compounds in cell culture and animal models. It is vital to know how much boron is taken up by tumor cells, how much of the boron is retained by the cells, and where the boron is localized within the cells before a decision can be made about using a compound for clinical trials. Ion microscopy is a very powerful technique which can be used for evaluating the uptake, localization, selectivity, and retention of boron in cell cultures and animal models.
KeywordsBoron Neutron Capture Therapy Tumor Mast Cell Boron Uptake U251 Human Glioma Cell Polished Silicon Wafer
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