Phylogenetic Studies of Campylobacter jejuni Using Arbitrary Primer-PCR Fingerprinting

  • Alexandro C. T. Carvalho
  • Guillermo M. Ruiz-Palacios


Over the years several typing systems have been developed as tools for the study of the epidemiology of Campylobacter to identify common sources and characterize outbreaks. Until now, typing systems have been based on the use of heat-labile (Lior)3 and heat-stable (Penner)8 antigens. However, these systems are restricted to reference centers, and there are an important number of non-typable strains, mainly among those were isolated in developing countries or from domestic animals. In addition, these typing methods have inherent limitations to differentiate strains with the same serotype. Recently developed methods include ribotyping5, electrophoresis of whole bacterial proteins, pulse field gel electrophoresis of total DNA, and restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the flagella gene4,2, which have proved to be useful to determine relatedeness between strains of C. jejuni isolates from different sources and origins. Most of these methods are slow, cumbersome and expensive. In this study, we used the arbitrary primed PCR technique9, with which it is possible to generate polymorphism of total genomic DNA of Campylobacter, to perform hierarchical analysis of strains, and we were able to identify not only strains from the some source in outbreaks, but also to group strains from different geographic areas and to distinguish between human and non-human isolates.


Nucleic Acid Research Group Strain Nucleic Acid Concentration Guanidium Thiocyanate Flagellum Gene4 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Barrett T.J., Patton C.M., Morris G.K. (1985) Lab. Medicine, 19, 96–102.Google Scholar
  2. 2.
    Burnens A.P., Wagner J., Lior H., Nicolet J., Frey J. (1995) Epidemiol. Infection, 114, 423–431.CrossRefGoogle Scholar
  3. 3.
    Lior H., Woodward D.L., Edgar J.A., Laroche L.J., Gill P. (1982) J. Clin. Microbiol, 15, 761–768.PubMedGoogle Scholar
  4. 4.
    Nachamkin I., Bohachick K., Patton C.M. (1993) J. Clin. Microbiol, 31, 1531–1536.PubMedGoogle Scholar
  5. 5.
    Owen R.J., Costas M., Sloss L., Bolton F.J. (1988) J. Applied Bacteriol, 65, 69–78.CrossRefGoogle Scholar
  6. 6.
    Owen R.J., Desai M., Garcia S. (1993) Res. Microbiol, 144, 709–720.PubMedCrossRefGoogle Scholar
  7. 7.
    Pitcher D.G., Saunders N.A., Owen R.J. (1989) Lett Appl. Microbiol, 8, 151–156.CrossRefGoogle Scholar
  8. 8.
    Penner L.J. & Hennessey J.N. (1980) J. Clin. Microbiol, 12, 732–737.PubMedGoogle Scholar
  9. 9.
    Welsh J. & McClelland M. (1990) Nucleic Acids Research, 18, 7213–7218.PubMedCrossRefGoogle Scholar
  10. 10.
    Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A., Tingey S.V. (1990) Nucleic Acids Research, 18, 6531–6535.PubMedCrossRefGoogle Scholar
  11. 11.
    Williams J.G.K., Hanafey M.K., Rafalski J.A., Tingey S.V. (1993) Methods Enzymol, 218, 704–740.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Alexandro C. T. Carvalho
    • 1
  • Guillermo M. Ruiz-Palacios
    • 1
  1. 1.Department of Infectious DiseasesNational Institute of NutritionDel. Tlalpan, Mexico CityMexico

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