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Differentiation within Campylobacter jejuni and C. coli by PCR-RFLP of the Intergenic Region between the flaA and flaB Genes

  • M. Linton
  • L. M. Lawrence
  • J. T. M. McGuiggan

Abstract

Campylobacter is now recognised as one of the most common causes of bacterial gastroenteritis in humans in developed countries. The most frequently isolated organisms from these cases are C. jejuni and C. coli, with foods of animal origin thought to be the main sources of infection. However, the epidemiology of Campylobacter has been hampered by a lack of discriminatory techniques which are essential to identify vehicles of infection to humans, and sources of contamination of foods. The aim of this project was to investigate the DNA sequence divergence within the intergenic sequence between the flagellin genes, fla A and flaB, as a means of rapidly identifying sub-species within C. jejuni and C. coli.

Keywords

Intergenic Region Restriction Profile Intergenic Sequence Intergenic Space Region Flagellin Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Candrian U, B Furrer, C Höfelein and J Lüthy (1991) Appl and Environ Microbiol 57 955–961.Google Scholar
  2. 2.
    Wegmüller B, J Lüthy and U Candrian (1993) Appl Environ Microbiol 59 2161–2165.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • M. Linton
    • 1
  • L. M. Lawrence
    • 1
    • 2
  • J. T. M. McGuiggan
    • 1
  1. 1.Food Science DivisionDepartment of Agriculture for Northern IrelandBelfastNorthern Ireland, UK
  2. 2.Agriculture and Food Science CentreThe Queen’s University of BelfastBelfastN. Ireland, UK

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