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Use of a Candle Jar for Incubating Campylobacter jejuni

  • M. R. El-Sherbeeny

Abstract

The candle jar is one of the systems used to provide an atmosphere of approximately 17–19% oxygen and 2–4% CO2 2 suitable for the isolation of Campylobacters. Previous studies have compared the candle jar with other systems for the isolation of Campylobacters 1,3,4, The purpose of this study was to use the candle jar for optimising the growth of C.jejuni by changing the number of plates in the candle jar and by the use of selective media, with or without FBP supplement, at different incubation temperatures (37 and 42°C). A standard inoculum from normal faeces was added to the plated to mimic natural feacal flora and in order to reduce the oxygen tension in the candle jar.

Keywords

Oxygen Tension Incubation Temperature Oxygen Atmosphere Test Strain Control Plate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Boek, M.D. (1981) In: Campylobacter; Epidemiology, Pathogenesis and Biochemistry. (D.G. Newell ed) MPT Press Ltd, Lancaster, UK, pp 71–72.Google Scholar
  2. 2.
    Luechtenfeld N.W. (1981) Lab. Med., 12; 481–487.Google Scholar
  3. 3.
    Leuchtenfeld N.W., Relier L.B., Blaser M.J. and Wang W.L.L. (1982) J. Clin. Microbiol., 15; 53–57.Google Scholar
  4. 4.
    Wang, W.L.L., Luechtenfeld, N.W. Blaser M.J. and Keller L.B. (1982) J. Clin. Microbiol., 16; 291–294.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • M. R. El-Sherbeeny
    • 1
  1. 1.Nutrition InstituteCairoEgypt

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