Detection of Antigenic Cross-Reactivity Between Helicobacter Pylori and other Bacteria by Inhibition Elisa

Abstract

Helicobacter pylori is firmly established as a major cause of gastritis and peptic ulcer.⁸ Traditional methods of diagnosis rely on the detection of organisms from antral biopsy by histology, culture or rapid urease test. Non-invasive methods include radio-labelled urea breath tests and serology. Raised IgG response is ubiquitous in H. pylori infected individuals³ and serves as a good diagnostic tool. Enzyme-linked immurosorbent assay (ELISA) is an accurate, cost-effective, rapid and simple method of screening large numbers of sera for epidemiological investigation. In this study, an in-house ELISA was developed which employed ultra-centrifuged sonicated antigen (rich in urease), and the results were compared with biopsy findings. Further, we evaluated the quality of this ELISA by performing inhibition ELISA with Campylobacter jejuni, Salmonella species, Escherichia coli, Proteus mirabilis and H. pylori.