Expression of Campylobacter Jejuni flaA Epitopes within a Modified Salmonella Flagellin Expressed in Salmonella Enteritidis
The potential for using Salmonella as a vector for the delivery and presentation of heterologous antigens in the development of multivalent vaccines has been considered widely. The flagella of Salmonella spp. are highly immunogenic and antigenically variable. Detailed molecular analyses have identified key epitopic regions within the gene encoding the flagellin, filC, of several Salmonella spp. and deletion/insertional mutants have demonstrated the potential of these regions to act as vectors for foreign antigens (for a review see Stocker and Newton10). In a number of vaccine development experiments, re-combinant flagellin has been expressed by live attenuated Salmonella vaccine candidates which continue to express active flagellae and present the heterologous epitopes to the immune system. In some instances protective responses have been generated5. Other antigens expressed by Salmonella spp. such as the type IV-like SEF14 fimbriae of S. enteritidis 11 may also be useful as molecular vectors. Recently candidate live attenuated S. enteritidis vaccine candidates have been developed successfully for use in the chicken2,3. Thus, a longer term aim is the generation and testing of multivalent S. enteritidis vaccines expressing heterologous epitopes. The flagellin of Campylobacter jejuni is well characterised at the molecular level both in terms of its genetics and immunogenicity6,7. Its role as a virulence and/or colonisation factor has been discussed widely. The C. jejuni flagellin is therefore considered an appropriate antigen to express as a multivalent vaccine. Moreover flagellin is immundominant at the systemic and mucosal levels during experimental oral infection of chickens with C. jejuni 1 In this short communication we present the details of the construction of a recombinant S. muenchen: C. jejuni flagellin.
KeywordsVaccine Candidate Allelic Exchange Colonisation Factor Heterologous Antigen Multivalent Vaccine
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