Lipopolysaccharides from Campylobacter Jejuni Strains Associated with the Onset of the Guillain-Barré and Miller-Fisher Syndromes

  • Gerald O. Aspinall
  • Henrianna Pang
  • John L. Penner
  • Jeong E. Nam Shin


The Miller-Fisher syndrome (MFS) is an infrequent variation of the more common neuropathy known as the Guillain-Barré syndrome (GBS). The onset of GBS is often preceded by intestinal infections with Campylobacter jejuni. Kuroki et al. 4 reported that 10 of their 14 cases of GBS were preceded by diarrhoea due to C. jejuni of serotype 0:19. In previous investigations LPS from two GBS-related isolates, OH4382 and OH4384 typed as C. jejuni O:19, were compared with that from the 0:19 serostrain1–3 The high Mr components were indistinguishable, with polysaccharide O chains consisting of disaccharide repeating units of an amidated hyaluronic acid. The core oligosaccharide (OS) regions in the O:19 se-rostrain consisted of molecules with terminal units that mimic human gangliosides GDla and GM1. The core OS regions of the OH4382 and OH4384 differed in structure, but contained a terminal trisaccharide epitope, Neu5Acα2→8Neu5Acα2→3Galβ, in mimicry of the human ganglioside GD3. We report here an examination of the LPS of a C. jejuni strain PG836 isolated from a patient with MFS at the Rhode Island Hospital, Providence, R.I., U.S.A. Serotyping and immunoblotting evidence revealed that the isolate was not of serotype O:19, but belonged to a different serotype (O:10).


Hyaluronic Acid Nuclear Magnetic Resonance Spectroscopy Core Oligosaccharide Rhode Island Hospital Strain PG836 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Aspinall, G.O., Fujimoto, S., McDonald, A.G., Pang, H., Kurjanczyk, L.A. and Penner, J.L. (1994) Infect. Immun. 62, 2122–2125.PubMedGoogle Scholar
  2. 2.
    Aspinall, G.O., McDonald, A.G., Pang, H., Kurjanczyk, L.A. and Penner, J.L. (1994) Biochemistry 33, 241–249.PubMedCrossRefGoogle Scholar
  3. 3.
    Aspinall, G.O., McDonald, A.G. and Pang, H. (1994) Biochemistry 33, 250–255.PubMedCrossRefGoogle Scholar
  4. 4.
    Kuroki, S., Saida, T., Nukina, M., Haruta, T., Yoshioka, M., Kobayashi, Y. and Nakanishi, H. (1993) Ann. Neurol. 33, 243–247.PubMedCrossRefGoogle Scholar
  5. 5.
    Yuki, N., Taki, T., Takahashi, M., Saito, K., Yoshino, Tai, T., Handa, S. and Miyatake T. (1994) Ann. Neurol. 36, 791–793.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Gerald O. Aspinall
    • 1
  • Henrianna Pang
    • 2
  • John L. Penner
    • 3
  • Jeong E. Nam Shin
    • 1
  1. 1.Department of ChemistryYork UniversityNorth York, TorontoCanada
  2. 2.Department of Molecular and Medical GeneticsUniversity of TorontoTorontoCanada
  3. 3.Department of MicrobiologyUniversity of TorontoTorontoCanada

Personalised recommendations