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Enzyme-Linked Immunosorbent Assays (ELISAs) to Detect Botulinum Toxins Using High Titer Rabbit Antisera

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Botulinum and Tetanus Neurotoxins

Abstract

Botulinum toxin types A through G are potent neurotoxic proteins produced by heterogeneous clostridia.1–5 Conventional testing for botulinum toxin is done by mouse bioassay. Toxin type is determined by the mouse protection assay using specific botulinum toxin antisera.6 The mouse bioassay is a useful test allowing a direct and sensitive determination of LD50 and antibody titers. However the test involves tedious manipulations and the use of live animals. Notermans et al.7 pioneered the development of enzyme-linked immunosorbent assays (ELISAs) for toxins A, B, and E. Since then, numerous radioimmunoassays8 and ELISAs9–18 for detecting botulinum toxins have been published. All are immunoassays, and therefore the results are not strictly comparable to the biological activity measured in the mouse bioassay. Since antibodies and toxin standards needed to perform ELISAs are not generally available, these tests cannot readily be duplicated by other laboratories.

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References

  1. Hauschild AHW. Clostridium botulinum toxins. Int. J. Food Microbiol. 1990; 10:113–24.

    Google Scholar 

  2. Lee WH, Riemann H. Correlation of toxic and non-toxic strains of Clostridium botulinum by DNA composition and homology. J. Gen. Microbiol. 1970; 60: 117–23.

    Article  PubMed  CAS  Google Scholar 

  3. Lee WH, Riemann H. The genetic relatedness of Clostridium botulinum strains. J. Gen. Microbiol. 1970; 64: 85–90.

    Article  PubMed  CAS  Google Scholar 

  4. Suen JC, Hatheway CL, Steigerwalt AG et al. Genetic confirmation of identities of neurotoxigenic Clostridium barati and Clostridium butyricum implicated as agents of infant botulism. J. Clin. Microbiol. 1988; 26: 2191–92.

    PubMed  CAS  Google Scholar 

  5. Suen JC, Hatheway CL, Steigerwalt AG et al. Clostridium argentinense sp. nov.: a genetically homogenous group composed of all strains of Clostridium botulinum toxin type G and some nontoxigenic strains previously identified as Clostridium subterminale or Clostridium hastiforme. Int. J. System. Bacteriol. 1988; 38: 375–81.

    Article  Google Scholar 

  6. Kautter DA, Lynt RK. Clostridium botulinum In: Speck ML, ed. Compendium of methods for the microbiological examination of foods. Washington, DC: American Public Health Assoc., 1976: 424–36.

    Google Scholar 

  7. Notermans S, Hagenaars M, Kozaki S. The enzyme-linked immunosorbent assay (ELISA) for the detection and determination of Clostridium botulinum A, B, and E. Methods Enzymol. 1982; 84: 223–38.

    Article  CAS  Google Scholar 

  8. Ashton AC, Growther JS, Dolly JD. A sensitive and useful radioimmunoassay for neurotoxin and its hemagglutinin complex for Clostridium botulinum. Toxicon. 1985; 23: 235–46.

    CAS  Google Scholar 

  9. Dezfulian M, Hatheway CL, Yolken RH et al. Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type A and B toxins in stool samples of infants with botulism. J. Clin. Microbiol. 1984; 20: 379–83.

    PubMed  CAS  Google Scholar 

  10. Ferreira JL, Hamdy MK, Herd ZL et al. Monoclonal antibody for the detection of Clostridium botulinum type A toxin. Mol. Cell. Probes. 1987; 1: 337–45.

    Article  PubMed  CAS  Google Scholar 

  11. Ferreira JL, Hamdy MK, McCay SG et al., Monoclonal antibody to type F Clostridium botulinum toxin. Appl. Environ. Microbiol. 1990; 56: 808–11.

    PubMed  CAS  Google Scholar 

  12. Gibson AM, Modi NK, Roberts TA et al. Evaluation of a monoclonal antibody-based immunoassay for detecting type A Clostridium botulinum toxin produced in pure culture and an inoculated model cured meat system. J. Appl. Bacteriol. 1987; 63: 217–26.

    Article  PubMed  CAS  Google Scholar 

  13. Gibson AM, Modi NK, Roberts TA et al. Evaluation of a monoclonal antibody based immunoassay for detecting type B Clostridium botulinum toxin produced in pure culture and an inoculated cured meat system. J. Appl. Bacteriol. 1988; 64: 285–91.

    Article  PubMed  CAS  Google Scholar 

  14. Henning VK, Meyer H, Cremer J. Schnellnachweis der Clostridium botulinum-Toxine A and B mittels Enzym-Immuno-Assay (ELISA). Arch. Lebensmittelhyg. 1991; 42: 49–71.

    Google Scholar 

  15. Kamata Y, Kozaki S, Nagai T et al. Evaluation of ELISA techniques for titration of monoclonal antibodies against botulinum toxin. Jpn. J. Vet. Sci. 1986; 48: 909–14.

    Article  CAS  Google Scholar 

  16. Michalik M, Grzybowski J, Ligieza J et al. Enzyme-linked immunosorbent assay (ELISA) for the detection and differentiation of Clostridium botulinum toxins type A and B. J. Immunol. Meth. 1986; 93: 225–30.

    Article  CAS  Google Scholar 

  17. Shone CP, Wilton-Smith P, Appleton N et al. Monoclonal antibody-based immunoassay for type A Clostridium botulinum toxin is comparable to the mouse bioassay. Appl. Environ. Microbiol. 1985; 50: 63–7.

    PubMed  CAS  Google Scholar 

  18. Wang YC, Sugiyama H. An ELISA for screening and detecting botulinum toxins (A, B, and E). Chin. J. Microbiol. Immunol 1986; 6: 253–56.

    CAS  Google Scholar 

  19. DasGupta BR. Structure and structure function relation of botulinum neurotoxins. In: Lewis Jr. GE ed. Biomedical aspects of botulism. New York, NY: Academic Press, 1981: 1–20.

    Google Scholar 

  20. Onishi I, Sugii S, Sakaguchi G. Oral toxicities of Clostridium botulinum toxins in response to molecular size. Infect. Immun 1977; 16: 107–9.

    Google Scholar 

  21. Sakaguchi G, Onishi I, Kozaki S. Purification and oral toxicities of Clostridium botulinum progenitor toxins. In: Lewis Jr. GE ed., Biomedical aspects of botulism. Academic Press, New York, NY: 1981: 21–34.

    Google Scholar 

  22. Anderson Jr. JH, Lewis Jr. GE. Clinical evaluation of botulinum toxoids. In: Lewis Jr. GE ed. Biomedical aspects of botulism, New York, NY: Academic Press, 1981: 233–46.

    Google Scholar 

  23. Moberg LT, Sugiyama H Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex. Appl. Environ. Microbiol. 1978; 35: 878–80.

    PubMed  CAS  Google Scholar 

  24. Lowry OH, Rosebrough NJ, Farr AL et al. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951; 193: 265–75.

    PubMed  CAS  Google Scholar 

  25. Gimenez JA, Sugiyama H. Simplified purification method for Clostridium botulinum type E toxin. Appl. Environ. Microbiol. 1987; 53: 2827–30.

    CAS  Google Scholar 

  26. Gimenez JA, Sugiyama H. Comparison of toxins of Clostridium butyricum and Clostridium botulinum type E. Infect. Immun. 1988; 56: 926–29.

    CAS  Google Scholar 

  27. Woody M, DasGupta BR. E.fect of tetranitromethane on the biological activities of botulinum neurotoxin types A, B and E. Mol. Cell. Biochem. 1989; 85: 159–69.

    CAS  Google Scholar 

  28. Sakaguchi G, Sakaguchi S, Kurazono H et al. Persistence of specific antigenic protein in the serum of chickens given intravenous botulinum toxin type B, C, D, E, or F. FEMS Microbiol. Let. 1987; 43: 355–9.

    CAS  Google Scholar 

  29. De Paiva A, Dolly JO. Light chain of botulinum neurotoxin is active in the mammalian motor nerve terminals when delivered via liposomes. FEBS Let. 1990; 277: 171–7.

    Article  Google Scholar 

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Author information

Author notes

  1. Elisa L. Elliot

    Present address: Food and Drug Administration, 200 C St. S.W., Washington, DC., USA

Authors and Affiliations

  1. Food Safety and Inspection Service, United States Department of Agriculture, Building 322, BARC-East 10300 Baltimore Ave., Beltsville, MD, 20705-2350, USA

    Gerri M. Ransom, Wei H. Lee, Elisa L. Elliot & Charles P. Lattuada

Authors
  1. Gerri M. Ransom
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  2. Wei H. Lee
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  3. Elisa L. Elliot
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  4. Charles P. Lattuada
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Editor information

Editors and Affiliations

  1. University of Wisconsin, Madison, Wisconsin, USA

    Bibhuti R. DasGupta

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© 1993 Springer Science+Business Media New York

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Ransom, G.M., Lee, W.H., Elliot, E.L., Lattuada, C.P. (1993). Enzyme-Linked Immunosorbent Assays (ELISAs) to Detect Botulinum Toxins Using High Titer Rabbit Antisera. In: DasGupta, B.R. (eds) Botulinum and Tetanus Neurotoxins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9542-4_49

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  • DOI: https://doi.org/10.1007/978-1-4757-9542-4_49

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9544-8

  • Online ISBN: 978-1-4757-9542-4

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