Low Resolution Model of Botulinum Neurotoxin Type A

  • Raymond C. Stevens

Abstract

The function of botulinum neurotoxin is rapidly being elucidated by biochemical and molecular biology techniques. Although this information is critical to understanding the function of the toxin, the 3-dimensional structure of the neurotoxin is necessary to correlate the structure and function. To better understand the function of botulinum neurotoxin, the three-dimensional structure determination of the neurotoxin is being undertaken using the technique of X-ray diffraction. The technique of dynamical light scattering is being used to aid the crystallographic investigation of the 3-dimensional structure determination of serotype A.

Keywords

Dynamical Light Scattering Diphtheria Toxin Molecular Biology Technique Botulinum Neurotoxin Clostridium Botulinum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Choe S, Bennett MJ, Fujii G, Curmi MG, Kantardjieff KA, Collier RJ, Eisenberg D. The crystal structure of diphtheria toxin. Nature 1992; 357: 216–222.PubMedCrossRefGoogle Scholar
  2. 2.
    DasGupta BR, Rasmussen S. Purification and amino acid composition of Clostridium botulinum type E neurotoxin. Toxicon 1983; 535–545.Google Scholar
  3. 3.
    DasGupta BR, Sathyamoorthy V. Purification and amino acid composition of type A botulinum neurotoxin. Toxicon 1984; 21: 415–424.CrossRefGoogle Scholar
  4. 4.
    DasGupta BR, Woody M. Amino acid composition of Clostridium botulinum type B neurotoxin. Toxicon 1984; 22: 312–315.PubMedCrossRefGoogle Scholar
  5. 5.
    Kenney A, Claes P, Boss M. An advanced protein size detector. American Lab 1991; 23: 32D–32K.Google Scholar
  6. 6.
    Robinson JP, Schmid MF, Morgan DG, Chiu W. Three-dimensional structural analysis of tetanus toxin by electron crystallography. J Mol Biol 1988; 200: 367–375.PubMedCrossRefGoogle Scholar
  7. 7.
    Stevens RC, Evenson ML, Tepp W, DasGupta, BR. Crystallization and preliminary X-ray analysis of botulinum neurotoxin type A. J Mol Biol 1991; 222: 877–880.PubMedCrossRefGoogle Scholar
  8. 8.
    Warren SG, Edwards BFP, Evans DR, Wiley DC, Lipscomb WN. Aspartate transcarbamylase from Escherichia coli: electron density at 5.5A resolution. Proc Natl Acad Sci USA 1973; 70: 1117 1121.Google Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Raymond C. Stevens
    • 1
  1. 1.Department of Chemistry Gibbs Chemical LaboratoryHarvard UniversityCambridgeUSA

Personalised recommendations