Partial Characterization of Bovine Synaptosomal Proteins Adhered to By Botulinum and Tetanus Neurotoxins

  • Cara-Lynne Schengrund
  • Bibhuti R. DasGupta
  • Nancy J. Ringler


Binding of botulinum (BTx) and tetanus (TTx) neurotoxins to synaptic termini has been hypothesized to require a protein component(s)1–3 in addition to gangliosides of the Glb series4,5 (nomenclature described by Svennerhoim6). Results that we obtained in studies of inhibition by gangliosides of the binding of BTxA and TTx to ganglioside GT1b-coated plastic wells indicate that Glb gangliosides do not function as high affinity ligands for the neurotoxins. We observed that the concentration of ganglioside GT1b needed to block binding of either BTxA or TTx to GT1bcoated plastic wells was in the µM range.7 In contrast, when the same assay system was used to determine the concentration of ganglioside GM1 needed to block binding of cholera toxin to GM1-coated plastic wells, nM values were obtained.8


Ammonium Sulfate Cholera Toxin Electric Organ Tetanus Toxin High Affinity Ligand 
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  1. 1.
    K Stoeckel, M. Schwab, and H. Thoenen, Role of gangliosides in the uptake and retrograde axonal transport of cholera and tetanus toxin as compared to nerve growth factor and wheat germ agglutinin, Brain Res. 132: 273 (1977).PubMedCrossRefGoogle Scholar
  2. 2.
    E.J. Pierce, M.D. Davison, R.G. Parton, W.H. Habig, and D.R. Critchley, Characterization of tetanus toxin binding to rat brain membranes, Biochem. J. 236: 845 (1986).PubMedGoogle Scholar
  3. 3.
    E. Yavin and A. Nathan, Tetanus toxin receptors on nerve cells contain a trypsinsensitive component, Eur. J. Biochem. 154: 403 (1986).PubMedCrossRefGoogle Scholar
  4. 4.
    W.E. vanHeyningen, Gangliosides as membrane receptors for tetanus toxin, cholera toxin and serotonin, Nature, Lond. 249: 415 (1974).CrossRefGoogle Scholar
  5. 5.
    J. Holmgren, H. Elwing, P. Fredman, and L. Svennerholm, Polystyrene adsorbed gangliosides for investigation of the structure of the tetanus-toxin receptor, Eur. J. Biochem. 106: 371 (1980).PubMedCrossRefGoogle Scholar
  6. 6.
    L. Svennerholm, Ganglioside designation, Adv. Exp. Med. Biol. 125: 11 (1980).PubMedCrossRefGoogle Scholar
  7. 7.
    C-L. Schengrund, B.R. DasGupta, and N.J. Ringler, Binding of botulinum and tetanus neurotoxins to ganglioside GT1b and derivatives thereof, J. Neurochem. 57: 1024 (1991).PubMedCrossRefGoogle Scholar
  8. 8.
    C-L. Schengrund and N.J. Ringler, Binding of Vibrio cholera toxin and the heat-labile enterotoxin of Escherichia coli to GM1, derivatives of GM1, and nonlipidoligosaccharide polyvalent ligands, J. Biol. Chem. 264: 13233 (1989).PubMedGoogle Scholar
  9. 9.
    P.H. Lin, R. Selinfreund, and W. Wharton, Western blot detection of epidermal growth factor receptor from plasmalemma of culture cells using 125I-labeled epidermal growth factor, Anal. Biochem. 167: 128 (1987).PubMedCrossRefGoogle Scholar
  10. 10.
    C. Solsona, G. Egea, J. Blasi, C. Casanova, and J. Marsal, The action of botulinum toxin on cholinergic nerve terminals isolated from the electric organ ofTorpedo marmorata. Detection of a putative toxin receptor, J. Physiol. (Paris) 84: 174 (1990).Google Scholar
  11. 11.
    D.R. Critchley, W.H. Habig, and P.H. Fishman, Reevaluation of the role of ganglio-sides as receptors for tetanus toxin, J. Neurochem. 47: 213 (1986).PubMedCrossRefGoogle Scholar
  12. 12.
    A. Nathan and E. Yavin, Periodate-modified gangliosides enhance surface binding of tetanus toxin to PC12 pheochromocytoma cells, J. Neurochem. 53: 88 (1989).PubMedCrossRefGoogle Scholar
  13. 13.
    C-L. Schengrund, N.J. Ringler, and B.R. DasGupta, Adherence of botulinum and tetanus neurotoxins to synaptosomal proteins, Brain Res. Bull. (in press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Cara-Lynne Schengrund
    • 1
  • Bibhuti R. DasGupta
    • 2
  • Nancy J. Ringler
    • 1
  1. 1.Department of Biological ChemistryThe M.S. Hershey Medical CenterHersheyUSA
  2. 2.Department of Food Microbiology and Toxicology University of WisconsinMadisonUSA

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