Biosynthesis and Processing of Platelet Glycoproteins in Megakaryocytes

  • Alain Duperray
  • Rolande Berthier
  • Gérard Marguerie
Part of the Blood Cell Biochemistry book series (BLBI, volume 2)


Megakaryocytopoiesis is a differentiation process that may be defined as the sequential or coordinate expression of platelet proteins from a committed hemopoietic progenitor to platelets. The synthesis of these different proteins leads to the acquisition of functional properties related to platelet physiology. Some platelet glycoproteins function as membrane receptors; others are packaged in the platelet α-granules and are surface exposed or released after platelet activation. Since the platelet has a weak biosynthetic capacity (Kieffer et al., 1987), platelet glycoproteins originate from plasma or are synthesized by the platelet precursor, the megakaryocyte (MK). Thus, to understand the biochemistry of the platelet and the processes of MK maturation leading to platelet production, it is important to study the biosynthesis of platelet proteins in the MK. Only partial information is available on the synthesis of these glycoproteins, and little is known about how they are packaged into subcellular fractions.


Human Platelet Chronic Myeloid Leukemic Patient Platelet Glycoprotein Factor Versus Platelet Membrane Glycoprotein 
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  1. Avanzi, G. C., Lista, P., Giovinazzo, B., Miniero, R., Saglio, G., Benetton, G., Coda, R., Cattoretti, G., and Pegoraro, L., 1988, Selective growth response to IL-3 of a human leukaemic cell line with megakaryoblastic features, Br. J. Haematol. 69: 359–366.PubMedGoogle Scholar
  2. Behnke, O., 1968, An electron microscope study of the megakaryocyte of the rat bone marrow, J. Ultrastruct. Res. 24: 412–433.PubMedGoogle Scholar
  3. Belloc, F., Hourdillé, P., Fialon, P., Boisseau, M. R., and Soria, J., 1985, Fibrinogen synthesis by megakaryocytes rich human marrow cell concentrates, Thromb. Res. 38: 341–351.PubMedGoogle Scholar
  4. Belloc, F., Heilmann, E., Combrie, R., Boisseau, M. R., and Nurden, A. T., 1987, Protein synthesis and storage in human platelets: A defective storage of fibrinogen in platelets in Glanzmann’s thrombasthenia, Biochim. Biophys. Acta 925: 218–225.PubMedGoogle Scholar
  5. Bennett, J. S., and Vilaire, G., 1979, Exposure of platelet fibrinogen receptors by ADP and epinephrine, J. Clin. Invest. 64: 1393–1400.PubMedGoogle Scholar
  6. Berkow, R. L., Straneva, J. E., Bruno, E. D., Beyer, G. S., Burgess, J. S., and Hoffman, R., 1984, Isolation of human megakaryocytes by density centrifugation and counterflow centrifugal elutriation, J. Lab. Clin. Med. 103: 811–818.PubMedGoogle Scholar
  7. Berndt, M. C., Gregory, C., Kabral, A., Zola, H., Fournier, D., and Castaldi, P. A., 1985, Purification and preliminary characterization of the glycoprotein Ib complex in the human platelet membrane, Eur. J. Biochem. 151: 637–649.PubMedGoogle Scholar
  8. Berridge, M. V., Fraser, J. K., Carter, J. M., and Lin, F. K., 1988, Effects of recombinant human erythropoietin on megakaryocytes and on platelet production in the rat, Blood 72: 970–977.PubMedGoogle Scholar
  9. Berthier, R., Douady, F., Kaufmann, A., Arvieux, J., Michallet, M., Sotto, J. J., Lajmanovich, A., Schweitzer, A., Chagnon, E., Thevenon, D., and Hollard, D., 1982, Purification and cryopreservation of granulomonocytic colony forming cells from the blood of patients with chronic granulocytic leukemia for autologous transplantation, Exp. Hematol. 10: 578–586.PubMedGoogle Scholar
  10. Berthier, R., Marin, M., Duperray, A., Prenant, M., Newton, I., Schweitzer, A., Hollard, D., and Marguerie, G., 1987, Megakaryocytic development in liquid cultures of cryopreserved leukocyte stem cell concentrates from chronic myelogenous leukemia patients, Exp. Hematol. 15: 750–758.PubMedGoogle Scholar
  11. Berthier, R., Valiron, O., Troesch, A., Clemancey-Marcille, G., Schweitzer, A., and Hollard, D., 1989, Cryopreservation of human megakaryocytic progenitor cells (CFU-MK): Influence of culture conditions, Cryobiology 26: 265–272.PubMedGoogle Scholar
  12. Bonfanti, R., Furie, B. C., Furie, B., and Wagner, D., 1989, PADGEM (GMP140) is a component of WeibelPalade bodies of human endothelial cells, Blood 73: 1109–1112.PubMedGoogle Scholar
  13. Bonthron, D., On, E. C., Mitsock, L. M., Ginsburg, D., Handin, R. I., and Orkin, S. H., 1986, Nucleotide sequence of pre-pro-von Willebrand factor cDNA, Nucleic Acids Res. 14:.7125–7127.Google Scholar
  14. Bray, P. F., Rosa, J. P., Lingappa, V. R., Kan, Y. W., McEver, R. P., and Shuman, M. A., 1986, Biogenesis of the platelet receptor for fibrinogen: Evidence for separate precursors for glycoproteins IIb and IIIa, Proc. Natl. Acad. Sci. USA 83: 1480–1484.PubMedGoogle Scholar
  15. Bray, P. F., Rosa, J. P., Johnston, G., Shiu, D. T., Cook, R. G., Lau, C., Kan, Y. W., McEver, R. P., and Shuman, M. A., 1987, Platelet glycoprotein IIb. Chromosomal localization and tissue expression, J. Clin. Invest. 80: 1812–1817.PubMedGoogle Scholar
  16. Burgess, T. L., and Kelly, R. B., 1987, Constitutive and regulated secretion of proteins, Annu. Rev. Cell Biol. 3: 243–293.PubMedGoogle Scholar
  17. Cerveny, T. J., Fass, D. N., and Mann, K. G., 1984, Synthesis of coagulation factor V by cultured aortic endothelium, Blood 63: 1467–1474.PubMedGoogle Scholar
  18. Chesney, C. M., Pifer, D., and Colman, R. W., 1981, Subcellular localization and secretion of factor V from human platelets, Proc. Natl. Acad. Sci. USA 78: 5180–5184.PubMedGoogle Scholar
  19. Chesterman, C. N., and Berndt, M. C., 1986, Platelet and vessel wall interaction and the genesis of atherosclerosis, Clin. Haematol. 15: 323–353.PubMedGoogle Scholar
  20. Chiu, H. C., Schick, P. K., and Colman, R. W., 1985, Biosynthesis of factor V in isolated guinea pig megakaryocytes, J. Clin. Invest. 75: 339–346.PubMedGoogle Scholar
  21. Coller, B. S., Peerschke, E. I., and Sullivan, C. A., 1983, Studies with a murine monoclonal antibody that abolishes risocetin-induced binding of von Willebrand factor to platelets: Additional evidence in support of GPIb as a platelet receptor for von Willebrand factor, Blood 61: 99–110.PubMedGoogle Scholar
  22. Comp, P. C., Nixon, R. R., Cooper, M. R., and Esmon, C. T., 1984, Familial protein S deficiency is associated with recurrent thrombosis, J. Clin. Invest. 74: 2082–2088.PubMedGoogle Scholar
  23. Crabtree, G. R., and Kant, J. A., 1982, Organization of the rat T-fibrinogen gene: Alternative mRNA splice patterns produce the TA and TB(T’) chains of fibrinogen, Cell 31: 159–166.PubMedGoogle Scholar
  24. Cramer, E. M., Vainchenker, W., Vinci, G., Guichard, J., and Breton-Gorius, J., 1985, Gray platelet syndrome: Immunoelectron microscopic localization of fibrinogen and von Willebrand factor in platelets and megakaryocytes, Blood 66: 1309–1316.PubMedGoogle Scholar
  25. Cramer, E. M., Debili, N., Martin, J. F., Gladwin, A. M., Breton-Gorius, J., Harrison, P., Savidge, G. F., and Vainchenker, W., 1989, Uncoordinated expression of fibrinogen compared to thrombospondin and von Willebrand factor in maturing human megakaryocytes, Blood 73: 1123–1129.PubMedGoogle Scholar
  26. Damiani, G., Zocchi, E., Fabbi, M., Bargellesi, A., and Patrone, F., 1983, A monoclonal antibody to platelet glycoproteins IIb and IIIa complex: Its use in purifying human megakaryocytes from sternal bone marrow aspirates for immunofluorescence studies of la-like antigens, Exp. Hematol. 11: 169–177.PubMedGoogle Scholar
  27. De Alarcon, P. A., 1989, Megakaryocyte colony-stimulating factor (Mk-CSF): Its physiologic significance, Blood Cells 15: 173–184.PubMedGoogle Scholar
  28. Dessypris, E. N., Gleaton, J. H., and Armstrong, O. L., 1987, Effect of human recombinant erythropoietin on human marrow megakaryocyte colony formation in vitro. Br. J. Haematol. 65: 265–269.PubMedGoogle Scholar
  29. Duperray, A., Berthier, R., Chagnon, E., Ryckewaert, J. J., Ginsberg, M., Plow, E., and Marguerie, G., 1987, Biosynthesis and processing of platelet GPIIb-IIIa in human megakaryocytes, J. Cell Biol. 104: 1665 1673.Google Scholar
  30. Duperray, A., Troesch, A., Berthier, R., Chagnon, E., Frachet, P., Uzan, G., and Marguerie, G., 1989, Biosynthesis and assembly of platelet GPIIb-IIIa in human megakaryocytes: Evidence that assembly between pro-GPIIb and GPIIIa is a prerequisite for expression of the complex on the cell surface, Blood 74: 1603–1611.PubMedGoogle Scholar
  31. Fair, D. S., and Marlar, R. A., 1986, Biosynthesis and secretion of factor VII, protein C, protein S, and the protein C inhibitor from a human hepatoma cell line, Blood 67: 64–70.PubMedGoogle Scholar
  32. Fair, D. S., Marlar, R. A., and Levin, E. G., 1986, Human endothelial cells synthesize protein S, Blood 67: 1168–1171.PubMedGoogle Scholar
  33. Fitzgerald, L. A., Steiner, B., Rall, S. C., Lo, S., and Phillips, D. R., 1987, Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to “Integrin,” J. Biol. Chem. 262: 3936–3939.PubMedGoogle Scholar
  34. Fox, J. E. B., 1985, Identification of actin-binding protein as the protein linking the membrane skeleton to glycoproteins on platelet plasma membranes, J. Biol. Chem. 260: 11970–11977.PubMedGoogle Scholar
  35. Fraser, J. K., Tan, A. S., Lin, F. K., and Berridge, M. V., 1989, Expression of high-affinity binding sites for erythropoietin on rat and mouse megakaryocytes, Exp. Hematol. 17: 10–16.PubMedGoogle Scholar
  36. Gewirtz, A. M., Bruno, E., Elwell, J., and Hoffman, R., 1983, In vitro studies of megakaryocytopoiesis in thrombocytotic disorders of man, Blood 61: 384–389.PubMedGoogle Scholar
  37. Gewirtz, A. M., Keefer, M., Doshi, K., Annamalai, A. E., Chiu, H. C., and Colman, R. W., 1986, Biology of human megakaryocyte factor V, Blood 67: 1639–1648.PubMedGoogle Scholar
  38. Ginsberg, M. H., Loftus, J. C., Ryckewaert, J. J., Pierschbacher, M., Pytela, R., Ruoslathi, E., and Plow, E. F., 1987, Immunochemical and amino-terminal sequence comparison of two cytoadhesins indicates they contain similar or identical beta subunits and distinct alpha subunits, J. Biol. Chem. 262: 5437–5440.PubMedGoogle Scholar
  39. Grant, B. W., Nichols, W. L., Solberg, L. A., Yachimiak, D. J., and Mann, K. G., 1986, A radioimmunoassay useful for quantitating megakaryocyte growth in vitro, in Megakaryocyte Development and Function ( R. F. Levine, N. Williams, J. Levine, and B. L. Evatt, eds.), pp. 117–121, Alan R. Liss, Inc., New York.Google Scholar
  40. Greenberg, S. M., Rosenthal, D. S., Greeley, T. A., Tantravahi, R., and Handin, R. I., 1988, Characterization of a new megakaryocytic cell line: The Dami cell, Blood 72: 1968–1977.PubMedGoogle Scholar
  41. Handagama, P. J., George, J. N., Shuman, M. A., McEver, R. P., and Bainton, D. F., 1987, Incorporation of a circulating protein into megakaryocyte and platelet granules, Proc. Natl. Acad. Sci. USA 84: 861–865.PubMedGoogle Scholar
  42. Harmon, J. T., and Jamieson, G. A., 1986, Activation of platelets by a-thrombin is a receptor-mediated event, J. Biol. Chem. 261: 15928–15933.PubMedGoogle Scholar
  43. Henry, I., Uzan, G., Weil, D., Nicolas, H., Kaplan. J. C., Marguerie, G., Kahn, A., and Julien, C., 1984, The genes coding for An- Bß-, and -r-chains of fibrinogen map to 4q2, Am. J. Hum. Genet. 36: 760–768.Google Scholar
  44. Hibbin, J. A., Matutes, E., and Goldman, J. M., 1985, Proliferation in liquid culture of megakaryocytes from the blood of patients with primary myelofibrosis and other myeloproliferative disorders, Leuk. Res. 9: 1171–1179.PubMedGoogle Scholar
  45. Hoeprich, P., and Doolittle, R. F., 1983, Dimeric half-molecules of human fibrinogen are joined through disulfide bonds in an antiparallel orientation, Biochemistry 22: 2049–2055.PubMedGoogle Scholar
  46. Hourdillé, P., Fialon, P., Belloc, F., Namur, M., Boisseau, M. R., and Nurden, A. T., 1986, Megakaryocytes from the marrow of a patient with Glansmann’s thrombasthenia lacked GP IIb-IIIa complexes, Thromb. Haemostasis 56: 66–70.Google Scholar
  47. Hsu-Lin, S. C., Berman, C. L., Furie, B. C., August, D., and Furie, B., 1984, A platelet membrane protein expressed during platelet activation and secretion; studies using a monoclonal antibody specific for thrombin-activated platelets, J. Biol. Chem. 259: 9121–9126.PubMedGoogle Scholar
  48. Ishibashi, T., and Burstein, S. A., 1985, Separation of murine megakaryocytes and their progenitors on continuous gradients of percoll, J. Cell. Physiol. 125: 559–566.PubMedGoogle Scholar
  49. Ishibashi, T., Ruggeri, Z. M., Harker, L. A., and Burstein, S. A., 1986, Separation of human megakaryocytes by state of differentiation on continuous gradients of percoli: Size and ploidy analysis of cells identified by monoclonal antibody to glycoprotein IIb/IIIa, Blood 67: 1286–1292.PubMedGoogle Scholar
  50. Ishibashi, T., Koziol, J. A., and Burstein, S. A., 1987, Human recombinant erythropoietin promotes differentiation of murine megakaryocytes in vitro, J. Clin. Invest. 79: 286–289.PubMedGoogle Scholar
  51. Jackson, C. W., Brown, L. K., Somerville, B. C., Lyles, S. A., and Look, A. T., 1984, Two-color flow cytometric measurement of DNA distributions of rat megakarocytes in unfixed, unfractionated marrow cell suspensions, Blood 63: 768–778.PubMedGoogle Scholar
  52. Jackson, C. W., Steward, S. A., Brown, L. K., and Look, A. T., 1986, Inverse relationship between megakaryocyte buoyant density and maturity, Br. J. Haematol. 64: 33–43.PubMedGoogle Scholar
  53. Jackson, C. W., Steward, S. A., Hutson, N. K., and Ashmun, R. A., 1987, Interaction of ristocetin and bovine plasma with guinea pig megakaryocytes: A means to enrich megakaryocytes based on membrane rather than physical characteristics, Blood 69: 173–179.PubMedGoogle Scholar
  54. Jaffe, E. A., Hoyer, L. W., and Nachman, R. L., 1973, Synthesis of antihemophilic factor antigen by cultured human endothelial cells, J. Clin. Invest. 52: 2757–2764.PubMedGoogle Scholar
  55. Jandrot-Perrus, M., Mosesson, M. W., Denniger, M. H., and Ménaché, D., 1979, Studies of platelet fibrinogen from a subject with a congenital plasma fibrinogen abnormality (fibrinogen Paris I), Blood 54: 1109–1116.PubMedGoogle Scholar
  56. Jenkins, R. B., Nichols, W. L., Mann, K. G., and Solberg, L. A., 1986, CFU-M-derived human mega-karyocytes synthesize glycoproteins IIb and IIIa, Blood 67: 682–688.PubMedGoogle Scholar
  57. Jennings, L. K., and Phillips, D. R., 1982, Purification of glycoproteins IIb and III from human platelet plasma membranes and characterization of a calcium-dependent glycoprotein IIh-III complex, J. Biol. Chem. 257: 10458–10466.PubMedGoogle Scholar
  58. Jenny, R. J., Pittman, D. D., Toole, J. J., Kriz, R. W., Aldape, R. A., Hewick, R. M., Kaufman, R. J., and Mann, K. G., 1987, Complete cDNA and derived amino acid sequence of human factor V, Proc. Natl. Acad. Sci. USA 84: 4846–4850.PubMedGoogle Scholar
  59. Johnston, G. I., Cook, R. G., and McEver, R. P., 1989a, Cloning of GMP-140, a granule membrane protein of platelets and endothelium: Sequence similarity to proteins involved in cell adhesion and inflammation, Cell 56: 1033–1044.PubMedGoogle Scholar
  60. Johnston, G. I., Kurosky, A., and McEver, R. P., 1989b, Structural and biosynthetic studies of the granule membrane protein, GMP-140, from human platelets and endothelial cells, J. Biol. Chem. 264: 1816–1823.PubMedGoogle Scholar
  61. Judson, P. A., Anstee, D., and Clamp, J. R., 1982, Isolation and characterization of the major oligosaccharide of human platelet membrane glycoprotein GPIb, Biochem. J. 205: 81–90.PubMedGoogle Scholar
  62. Juvonen, E., 1988, Megakaryocyte colony formation in chronic myeloid leukemia and myelofibrosis, Leuk. Res. 12: 751–756.PubMedGoogle Scholar
  63. Kajigaya, S., Suda, T., Suda, J., Eguchi, M., Moroi, M., Sudo, T., Saito, M., and Miura, Y., 1988, Establishment of megakaryoblastic cell lines by coinfection of Abelson murine leukemia virus and recombinant SV40-retrovirus, Jpn. J. Cancer Res. 79: 726–733.PubMedGoogle Scholar
  64. Kane, W., and Davie, E. W., 1986, Cloning of a cDNA coding for human factor V, a blood coagulation factor homologous to factor VII and ceruloplasmin, Proc. Natl. Acad. Sci. USA 83: 6800–6804.PubMedGoogle Scholar
  65. Kane, W. H., Lindhout, M. J., Jackson, C. M., and Majerus, P. W., 1980, Factor Va-dependent binding of factor Xa to human platelets, J. Biol. Chem. 255: 1170–1174.PubMedGoogle Scholar
  66. Kane, W. H., Ichinose, A., Hagen, F. S., and Davie, E. W., 1987, Cloning of cDNAs coding for the heavy chain region and connecting region of human factor V, a blood coagulation factor with four types of internal repeats, Biochemistry 26: 6508–6514.PubMedGoogle Scholar
  67. Kant, J. A., Fornace, A. J., Saxe, D., Simon, M. I., McBride, O. W., and Crabtree, G. R., 1985, Evolution and organization of the fibrinogen locus on chromosome 4: Gene duplication accompanied by transposition and inversion, Proc. Natl. Acad. Sci. USA 82: 2344–2348.PubMedGoogle Scholar
  68. Kaplan, K. L., Broekman, M. J., Chernoff, A., Lesznik, G. R., and Drillings, M., 1979, Platelet a-granules proteins: Studies on release and subcellular localization, Blood 53: 604–618.PubMedGoogle Scholar
  69. Kieffer, N., Debili, N., Wield, A., Titeux, M., Henri, A., Mishal, Z., Breton-Gorius, J., Vainchenker, W., and Clemetson, K. J., 1986, Expression of platelet glycoprotein Iba in HEL cells, J. Biol. Chem. 261: 15854–15862.PubMedGoogle Scholar
  70. Kieffer, N., Guichard, J., Farcet, J. P., Vainchenker, and Breton-Gorius, J., 1987, Biosynthesis of major platelet proteins in human blood platelets, Eur. J. Biochem. 164: 189–195.PubMedGoogle Scholar
  71. Kieffer, N., Wautier, J. L., Coulombel, L., Titeux, M., Wautier, M. P., Vainchenker, W., Ruan, C., and BretonGorius, J., 1988, Uncoupling in the expression of platelet GPIIb/IIIa in human endothelial cells and K562 cells: Absence of immunologic crossreactivity between platelet GPIIb and the vitronectin receptor alpha chain, Blood 72: 1209–1215.PubMedGoogle Scholar
  72. Korrel, S. A. M., Clemetson, K. J., Van Halbeek, H., Kamerling, J. P., Sixma, J. J., and Vliegenthart, F. G., 1984, Structural studies on the 0-linked carbohydrate chains of human platelet glycocalicin, Eur. J. Biochem. 140: 571–576.PubMedGoogle Scholar
  73. Legaz, M. E., Schmer, G., Counts, R. B., and Davie, E. W., 1973, Isolation and characterization of human factor VIII (antihemophilic factor), J. Biol. Chem. 248: 3946–3955.PubMedGoogle Scholar
  74. Leven, R. M., Schick, P. K., and Budzynski, A. Z., 1985, Fibrinogen biosynthesis in isolated guinea pig megakaryocytes, Blood 65: 501–504.PubMedGoogle Scholar
  75. Levine, R. F., and Fedorko, M. F., 1976, Isolation of intact megakaryocytes from guinea pig femoral marrow, J. Cell Biol. 69: 159–172.PubMedGoogle Scholar
  76. Lingappa, V. R., 1989, Intracellular traffic of newly synthesized proteins. Current understanding and future prospects, J. Clin. Invest. 83: 739–751.PubMedGoogle Scholar
  77. Lopez, J. A., Chung, D. W., Fujikawa, K., Hagen, F. S., Papayannopoulou, T., and Roth, G. J., 1987, Cloning of the a chain of human platelet glycoprotein Ib: A transmembrane protein with homology to leucine-rich a2-glycoprotein, Proc. Natl. Acad. Sci. USA 84: 5615–5619.PubMedGoogle Scholar
  78. Lopez, J. A., Chung, D. W., Fujikawa, K., Hagen, F. S., Davie, E. W., and Roth, G. J., 1988, The a and 13 chains of human platelet glycoprotein Ib are both transmembrane proteins containing a leucine-rich amino acid sequence, Proc. Natl. Acad. Sci. USA 85: 2135–2139.PubMedGoogle Scholar
  79. Lozzio, C. B., and Lozzio, B. B., 1975, Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome, Blood 45: 321–334.PubMedGoogle Scholar
  80. Lundwall, A., Dackowski, W., Cohen, E., Shaffer, M., Mahr, A., Dahlback, B., Stenflo, J., and Wydro, R., 1986, Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation, Proc. Natl. Acad. Sci. USA 83: 6716–6720.PubMedGoogle Scholar
  81. Marguerie, G. A., Plow, E. F., and Edgington, T. S., 1979, Human platelets possess an inducible and saturable receptor specific for fibrinogen, J. Biol. Chem. 254: 5357–5363.PubMedGoogle Scholar
  82. Marguerie, G., Ginsberg, M. H., and Plow, E. F., 1987, The platelet fibrinogen receptor, in Platelets in Biology and Pathology Ill ( D. E. Macintyre and J. L. Gordon, eds.), pp. 95–125, Elsevier, New York.Google Scholar
  83. Martin, P., and Papayannopoulou, T., 1982, HEL cells: A new human erythroleukemia cell line with spontaneous and induced globin expression, Science 216: 1233–1235.PubMedGoogle Scholar
  84. Martin, S. E., Marder, V. J., Francis, C. W., and Barlow, G. H., 1981, Structural studies on the functional heterogeneity of von Willebrand protein polymers, Blood 57: 313–323.PubMedGoogle Scholar
  85. McEver, R. P., and Martin, M. N., 1984, A monoclonal antibody to a membrane glycoprotein binds only to activated platelets, J. Biol. Chem. 259: 9799–9804.PubMedGoogle Scholar
  86. McLeod, D. L., Shreeve, M. M., and Axelrad, A. A., 1980, Chromosome marker evidence for the bipotentiality of BFU-E, Blood 56: 318–322.PubMedGoogle Scholar
  87. Messner, H. A., Jamal, N., and Izaguirre, C., 1982, The growth of large megakaryocyte colonies from human bone marrow, J. Cell. Physiol. Suppl. 1: 45–51.PubMedGoogle Scholar
  88. Miletich, J. P., Jackson, C. M., and Majerus, P. W., 1978, Properties of the factor Xa binding site on human platelets, J. Biol. Chem. 253: 6908–6916.PubMedGoogle Scholar
  89. Mosesson, M. W., Homandberg, G. A., and Amrani, D. L., 1984, Human platelet fibrinogen gamma chain structure, Blood 63: 990–995.PubMedGoogle Scholar
  90. Nachman, R., Levine, R., and Jaffe, E. A., 1977, Synthesis of factor VIII antigen by cultured guinea pig megakaryocytes, J. Clin. Invest. 60: 914–921.PubMedGoogle Scholar
  91. Nakeff, A., Valeriote, F., Gray, J. W., and Grabske, R. J., 1979, Application of flow cytometry and cell sorting to megakaryocytopoiesis, Blood 53: 732–745.PubMedGoogle Scholar
  92. Nurden, A. T., 1987, Platelet membrane glycoproteins and their clinical aspects, in Thrombosis and Haemostat-sis ( M. Verstraete, J. Vermylen, H. R., Lijnen, and J. Arnout, eds.), pp. 93–125, International Society on Thrombosis and Haemostasis and Leuven Press, Leuven, Belgium.Google Scholar
  93. Ogura, M., Morishima, Y., Ohno, R., Kato, Y., Hirabayashi, N., Nagura, H., and Saito, H., 1985, Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome, Blood 66: 1384–1392.PubMedGoogle Scholar
  94. Ogura, M., Tanabe, N., Nishioka, J., Suzuki, K., and Saito, H., 1987, Biosynthesis and secretion of functional protein S by a human megakaryoblastic cell line (MEG-01), Blood 70: 301–306.PubMedGoogle Scholar
  95. Okumura, T., and Jamieson, G. A., 1976, Platelet glycocalicin. I. Orientation of glycoproteins of the human platelet surface, J. Biol. Chem. 251: 5944–5949.PubMedGoogle Scholar
  96. Okumura, T., Lombart, C., and Jamieson, G. A., 1976, Platelet glycocalcin. II. Purification and characterization, J. Biol. Chem. 251: 5950–5955.PubMedGoogle Scholar
  97. Okumura, T., Hasitz, M., and Jamieson, G. A., 1978, Platelet glycocalicin. Interaction with thrombin and role as thrombin receptor of the platelet surface, J. Biol. Chem. 253: 3435–3443.PubMedGoogle Scholar
  98. Papayannopoulou, T., Raines, E., Collins, S., Nakamoto, B., Tweeddale, M., and Ross, R., 1987, Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers, J. Clin. Invest. 79: 859–866.PubMedGoogle Scholar
  99. Phillips, D. R., and Agin, P. P., 1977a, Platelet plasma membrane glycoproteins. Evidence for the presence of nonequivalent disulfide bonds using nonreduced-reduced two-dimensional gel electrophoresis, J. Biol. Chem. 252: 2121–2126.PubMedGoogle Scholar
  100. Phillips, D. R., and Agin, P. P., 1977b, Platelet membrane defects in Glanzmann’s thrombasthenia: Evidence for decreased amounts of two major glycoproteins, J. Clin. Invest. 60: 535–545.PubMedGoogle Scholar
  101. Phillips, D. R., and Jakabova, M., 1977, Cat+-dependent protease in human platelets. Specific cleavage of platelet polypeptides in the presence of added Cat+, J. Biol. Chem. 252: 5602–5605.PubMedGoogle Scholar
  102. Phillips, D. R., Charo, I. F., Panse, L. V., and Fitzgerald, L. A., 1988, The platelet membrane glycoprotein IIb-IIIa complex, Blood 71: 831–843.PubMedGoogle Scholar
  103. Plow, E. F., and Ginsberg, M., 1981, Specific and saturable binding of plasma fibronectin to thrombin stimulated human platelet, J. Biol. Chem. 256: 9477–9482.PubMedGoogle Scholar
  104. Plow, E. F., Loftus, J. C., Levin, E. G., Fair, D. S., Dixon, S., Forsyth, J., and Ginsberg, M. H., 1986, Immunological relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells, Proc. Natl. Acad. Sci. USA 83: 6002–6006.PubMedGoogle Scholar
  105. Poncz, M., Eisman, R., Heidenreich, R., Silver, S. M., Vilaire, G., Surrey, S., Schartz, E., and Bennett, J. S., 1987, Structure of the platelet membrane glycoprotein IIb: Homology to the a subunits of the vitronectin and fibronectin membrane receptors, J. Biol. Chem. 262: 8476–8482.PubMedGoogle Scholar
  106. Rabellino, E. M., Nachman, R. L., Williams, N., Winchester, R. J., and Ross, G. D., 1979, Human megakaryocytes. I. Characterization of the membrane and cytoplasmic components of isolated marrow megakaryocytes, J. Exp. Med. 149: 1273–1287.PubMedGoogle Scholar
  107. Rosa, J. P., George, J. N., Bainton, D. F., Nurden, A. T., Caen, J. P., and McEver, R. P., 1987, Gray platelet syndrome. Demonstration of alpha granule membranes that can fuse with the cell surface, J. Cin. Invest. 80: 1138–1146.Google Scholar
  108. Rosa, J. P., Bray, P. F., Gayet, O., Johnston, G. I., Cook, R. G., Jackson, K. W., Shuman, M. A., and McEver, R. P., 1988, Cloning of glycoprotein IIIa cDNA from human erythroleukemia cells and localization of the gene to chromosome 17, Blood 72: 593–600.PubMedGoogle Scholar
  109. Roth, B. J., Sledge, G. W., Straneva, J. E., Brandt, J., Goheen, M., and Hoffman, R., 1988, Analysis of phorbol ester stimulated human megakaryocyte development, Blood 72: 202–207.PubMedGoogle Scholar
  110. Ruggeri, Z. M., Bader, R., and De Marco, L., 1982, Glanzmann thrombastenia: Deficient binding of von Willebrand factor to thrombin-stimulated platelets, Proc. Natl. Acad. Sci. USA 79: 6038–6041.PubMedGoogle Scholar
  111. Sakariassen, K. S., Bolhuis, P. A., and Sixma, J. J., 1979, Human blood platelet adhesion to artery subendothelium is mediated by factor VII-von Willebrand factor bound to subendothelium, Nature (London) 279: 636–638.Google Scholar
  112. Sanderson, R. J., and Bird, K., 1977, Cell separation by counterflow centrifugation, Methods Cell. Biol. 15: 114.Google Scholar
  113. San Miguel, J. F., Tavares de Castro, J., Matutes, E., Rodriguez, B., Polli, N., Zola, H., McMichael, A. J., Bollum, F. J., Thompson, D. S., Goldman, J. M., and Catovsky, D., 1985, Characterization of blast cells in chronic granulocytic leukaemia in transformation, acute myelofibrosis and undifferentiated leukaemia, Br. J. Haematol. 59: 297–309.PubMedGoogle Scholar
  114. Schwarz, H. P., Heeb, M. J., Wencel-Drake, J. D., and Griffin, J. H., 1985, Identification and quantification of protein S in human platelets, Blood 66: 1452–1455.PubMedGoogle Scholar
  115. Seigneurin, D., Champelovier, P., Mouchiroud, G., Berthier, R., Leroux, D., Prenant, M., McGregor, J., Starck, J., Morle, F., Micouin, C., Pietrantuono, A., and Kolodie, L., 1987, Human chronic myeloid leukemic cell line with positive Philadelphia chromosome exhibits megakaryocytic and erythroid characteristics, Exp. Hematol. 15: 822–832.PubMedGoogle Scholar
  116. Shelton-Inloes, B., Titani, K., and Sadler, J. E., 1986, cDNA sequences for human von Willebrand factor reveal five types of repeated domains and five possible protein sequence polymorphisms, Biochemistry 25:3164–3171.Google Scholar
  117. Shoff, P. K., and Levine, R. F., 1989, Elutriation for isolation of megakaryocytes, Blood Cells 15: 285–305.PubMedGoogle Scholar
  118. Silver, S. M., McDonough, M. M., Vilaire, G., and Bennett, J. S., 1987, The in vitro synthesis of polypeptides for the platelet membrane glycoproteins IIb and IIIa, Blood 69: 1031–1037.PubMedGoogle Scholar
  119. Sitar, G., 1984, Isolation of normal human megakaryocytes, Br. J. Haematol. 58: 465–472.PubMedGoogle Scholar
  120. Sitar, G., Borroni, R., and Pesce, G., 1986, Synthesis of thrombospondin and glycoprotein IIb-IIIa by a purified megakaryocyte human bone marrow culture, Haematologica 71: 389–393.PubMedGoogle Scholar
  121. Sledge, G. W., Giant, M., Jansen, J., Heerema, N. A., Roth, B. J., Goheen, M., and Hoffman, R., 1986, Establishment in long term culture of megakaryocytic leukemia cells (EST-IU) from the marrow of a patient with leukemia and a mediastinal germ cell neoplasm, Cancer Res. 46: 2155–2159.PubMedGoogle Scholar
  122. Solberg, L. A., Jamal, N., and Messner, H. A., 1985, Characterization of human megakaryocytic colony formation in human plasma, J. Cell. Physiol. 124: 67–74.PubMedGoogle Scholar
  123. Soria, J., Soria, C., Samama, M., Henschen, A., and Southan, C., 1982, Detection of fibrinogen abnormality in dysfibrinogenemia. Special report on fibrinogen Metz characterized by an amino acid substitution located at the peptide bond cleaved by thrombin, in Fibrinogen. Recent Biomedical Aspects, ( A. Henshen, H. Graeff, and F. Lottspeich, eds.), pp. 126–143, W. de Gruyter and Co., New York.Google Scholar
  124. Sporn, L. A., Chavin, S. I., Marder, V. J., and Wagner, D. D., 1985, Biosynthesis of von Willebrand protein by human megakaryocytes, J. Clin. Invest. 76: 1102–1106.PubMedGoogle Scholar
  125. Stenberg, P. E., McEver, R. P., Shuman, M. A., Jacques, Y. V., and Bainton, D., 1985, A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation, J. Cell Biol. 101: 880–886.PubMedGoogle Scholar
  126. Stern, D., Brett, J., Harris, K., and Nawroth, P., 1986, Participation of endothelial cells in the protein C-protein S anticoagulant pathway: The synthesis and release of protein S, J. Cell Biol. 102: 1971–1978.PubMedGoogle Scholar
  127. Suzuki, S., Argraves, W. S., Arai, H., Languino, L. R., Pierschbacher, M. D., and Ruoslathi, E., 1987, Amino-acid sequence of the vitronectin receptor a-subunit and comparative expression of adhesion receptor mRNAs, J. Biol. Chem. 262: 14080–14084.PubMedGoogle Scholar
  128. Tange, T., Nakahara, K., Mitani, K., Yamasaki, I., Yasuda, H., Tanaka, F., Mizuguchi, M., Oda, H., Yatomi, Y., Takanashi, R., Fujioka, S., Yamaguchi, K., and Urano, Y., 1988, Establishment of a human megakaryoblastic cell line (T-33) from chronic myelogenous leukemia in megakaryoblastic crisis, Cancer Res. 48: 6137–6144.PubMedGoogle Scholar
  129. Tarentino, A. L., and Maley, F., 1974, Purification and properties of an endo-3-N-acetylglucosaminidase from streptomyces griseus, J. Biol. Chem. 249: 811–817.PubMedGoogle Scholar
  130. Tartakoff, A. M., 1983, Perturbation of vesicular traffic with carboxylic ionophore monensin, Cell 32: 1026–1028.PubMedGoogle Scholar
  131. Titani, K., Kumar, S., Takio, K., Ericsson, L. H., Wade, R. D., Ashida, K., Walsh, K. A., Chopek, M. W., Sadler, J. E., and Fujikawa, K., 1986, Amino acid sequence of human von Willebrand factor, Biochemistry 25: 3171–3184.PubMedGoogle Scholar
  132. Titani, K., Takio, K., Banda, M., and Ruggeri, Z. M., 1987, Amino acid sequence of the von Willebrand factor-binding domain of platelet membrane glycoprotein Ib, Proc. Natl. Acad. Sci. USA 84: 5610–5614.PubMedGoogle Scholar
  133. Tomer, A., Harker, L. A., and Burstein, S. A., 1987, Purification of human megakaryocytes by fluorescenceactivated cell sorting, Blood 70: 1735–1742.PubMedGoogle Scholar
  134. Townsend, R. R., Hilliker, E., Li, Y. T., Laine, R. A., Bell, W. R., and Lee, Y. C., 1982, Carbohydrate structure of human fibrinogen. Use of 300 H-NMR to characterize glycosidase-treated glycopeptides, J. Biol. Chem. 257: 9704–9710.PubMedGoogle Scholar
  135. Tsuji, T., Tsunehisa, S., Watanabe, Y., Yamamoto, K., Tohyama, H., and Osawa, T., 1983, The carbohydrate moiety of human platelet glycocalicin. The structure of the major Ser/Thr-linked sugar chain, J. Biol. Chem. 258: 6335–6339.PubMedGoogle Scholar
  136. Uzan, G., Courtois, G., Stanckovic, Z., Crabtree, G. R., and Marguerie, G., 1986, Expression of the fibrinogen genes in rat megakaryocytes, Biochem. Biophys. Res. Commun. 140: 543–549.PubMedGoogle Scholar
  137. Uzan, G., Frachet, P., Lajmanovich, A., Prandini, M. H., Denarier, E., Duperray, A., Loftus, J., Ginsberg, M., Plow, E., and Marguerie, G., 1988, cDNA clones for human paltelet GPIIIb corresponding to mRNA of megakaryocytes and erythroleukemic cells: Evidence for an extensive homology to other Arg-Gly-Asp adhesion receptors, Eur. J. Biochem. 171: 87–93.Google Scholar
  138. Valiron, O., Clémancey-Marcille, G., Troesch, A., Schweitzer, A., Prenant, M., Bollard, D., and Berthier, R., 1988, Immunophenotype of blast cells in chronic myeloid leukemia, Leuk. Res. 12: 861–872.PubMedGoogle Scholar
  139. Verweij, C. L., Diergaarde, P. J., Hart, M., and Pannekoek, H., 1986, Full-length von Willebrand factor (vWF) cDNA encodes a highly repetitive protein considerably larger than the mature vWF subunit, EMBO J. 5: 1839–1847.PubMedGoogle Scholar
  140. Vicic, W., Lages, B., and Weiss, H., 1980, Release of human platelet factor V activity is induced by both collagen and ADP and is inhibited by aspirin, Blood 56: 448–455.PubMedGoogle Scholar
  141. Vinci, G., Tabilio, A., Deschamps, J. F., Van Haeke, D., Henri, A., Guichard, J., Tetteroo, P., and Landsorp, P. M., Hercend, T., Vainchenker, W., and Breton-Gorius, J., 1984, Immunological study of in vitro maturation of human megakaryocytes, Br. J. Haematol. 58: 589–605.Google Scholar
  142. Wagner, D. D., and Marder, V. J., 1984, Biosynthesis of von Willebrand protein by human endothelial cells: Processing steps and their intracellular localization, J. Cell Biol. 99: 2123–2130.PubMedGoogle Scholar
  143. Wagner, D. D., Olmsted, J. B., and Marder, V. J., 1982, Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells, J. Cell Biol. 95: 355–360.PubMedGoogle Scholar
  144. Walker, F. J., 1980, Regulation of activated protein C by a new protein. A possible function for protein S, J. Biol. Chem. 255: 5521–5524.PubMedGoogle Scholar
  145. Weinstein, R., Stemerman, M. B., Maclntyre, D. E., Steinberg, H. N., and Maciag, T., 1981, The morphological and biochemical characterization of a line of rat promegakaryoblasts, Blood 58: 110–121.PubMedGoogle Scholar
  146. Wilson, D. B., Salem, H. H., Mruk, J. S., Maruyama, I., and Majerus, P. W., 1983, Biosynthesis of coagulation factor V by a human hepatocellular carcinoma cell line, J. Clin. Invest. 73: 654–658.Google Scholar
  147. Worthington, R. E., and Nakeff, A., 1981, Thromboxane synthesis in megakaryocytes isolated by centrifugal elutriation, Blood 58: 175–178.PubMedGoogle Scholar
  148. Wyler, B., Bienz, D., Clemetson, K. J., and Luscher, E. F., 1986, Glycoprotein Ibß is the only phosphorylated major membrane glycoprotein in human platelets, Biochem. J. 234: 373–379.PubMedGoogle Scholar
  149. Yeo, E., Furie, B., and Furie, B., 1989, PADGEM protein in human erythroleukemia cells, Blood 73:722–728. Zimmerman, T. S., Ruggeri, Z. M., and Fulcher, C. A., 1983, Factor VIII/von Willebrand factor, in Progress in Hematology ( E. B. Brown, ed.), Vol. 3, pp. 279–309, Grune and Stratton, New York.Google Scholar
  150. Zimrin, A. B., Eisman, R., Vilaire, G., Schwartz, E., Bennet, J. S., and Poncz, M., 1988, Structure of platelet glycoprotein IIIa. A common subunit for two different membrane receptors, J. Clin. Invest. 81: 1470–1475.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Alain Duperray
    • 1
  • Rolande Berthier
    • 1
  • Gérard Marguerie
    • 1
  1. 1.DRF/Laboratory of HematologyUnité 217 INSERM, Center for Nuclear StudiesGrenoble CedexFrance

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