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Biosynthesis and Processing of Platelet Glycoproteins in Megakaryocytes

  • Chapter
Megakaryocytes, Platelets, Macrophages, and Eosinophils

Part of the book series: Blood Cell Biochemistry ((BLBI,volume 2))

Abstract

Megakaryocytopoiesis is a differentiation process that may be defined as the sequential or coordinate expression of platelet proteins from a committed hemopoietic progenitor to platelets. The synthesis of these different proteins leads to the acquisition of functional properties related to platelet physiology. Some platelet glycoproteins function as membrane receptors; others are packaged in the platelet α-granules and are surface exposed or released after platelet activation. Since the platelet has a weak biosynthetic capacity (Kieffer et al., 1987), platelet glycoproteins originate from plasma or are synthesized by the platelet precursor, the megakaryocyte (MK). Thus, to understand the biochemistry of the platelet and the processes of MK maturation leading to platelet production, it is important to study the biosynthesis of platelet proteins in the MK. Only partial information is available on the synthesis of these glycoproteins, and little is known about how they are packaged into subcellular fractions.

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References

  • Avanzi, G. C., Lista, P., Giovinazzo, B., Miniero, R., Saglio, G., Benetton, G., Coda, R., Cattoretti, G., and Pegoraro, L., 1988, Selective growth response to IL-3 of a human leukaemic cell line with megakaryoblastic features, Br. J. Haematol. 69: 359–366.

    PubMed  CAS  Google Scholar 

  • Behnke, O., 1968, An electron microscope study of the megakaryocyte of the rat bone marrow, J. Ultrastruct. Res. 24: 412–433.

    PubMed  CAS  Google Scholar 

  • Belloc, F., Hourdillé, P., Fialon, P., Boisseau, M. R., and Soria, J., 1985, Fibrinogen synthesis by megakaryocytes rich human marrow cell concentrates, Thromb. Res. 38: 341–351.

    PubMed  CAS  Google Scholar 

  • Belloc, F., Heilmann, E., Combrie, R., Boisseau, M. R., and Nurden, A. T., 1987, Protein synthesis and storage in human platelets: A defective storage of fibrinogen in platelets in Glanzmann’s thrombasthenia, Biochim. Biophys. Acta 925: 218–225.

    PubMed  CAS  Google Scholar 

  • Bennett, J. S., and Vilaire, G., 1979, Exposure of platelet fibrinogen receptors by ADP and epinephrine, J. Clin. Invest. 64: 1393–1400.

    PubMed  CAS  Google Scholar 

  • Berkow, R. L., Straneva, J. E., Bruno, E. D., Beyer, G. S., Burgess, J. S., and Hoffman, R., 1984, Isolation of human megakaryocytes by density centrifugation and counterflow centrifugal elutriation, J. Lab. Clin. Med. 103: 811–818.

    PubMed  CAS  Google Scholar 

  • Berndt, M. C., Gregory, C., Kabral, A., Zola, H., Fournier, D., and Castaldi, P. A., 1985, Purification and preliminary characterization of the glycoprotein Ib complex in the human platelet membrane, Eur. J. Biochem. 151: 637–649.

    PubMed  CAS  Google Scholar 

  • Berridge, M. V., Fraser, J. K., Carter, J. M., and Lin, F. K., 1988, Effects of recombinant human erythropoietin on megakaryocytes and on platelet production in the rat, Blood 72: 970–977.

    PubMed  CAS  Google Scholar 

  • Berthier, R., Douady, F., Kaufmann, A., Arvieux, J., Michallet, M., Sotto, J. J., Lajmanovich, A., Schweitzer, A., Chagnon, E., Thevenon, D., and Hollard, D., 1982, Purification and cryopreservation of granulomonocytic colony forming cells from the blood of patients with chronic granulocytic leukemia for autologous transplantation, Exp. Hematol. 10: 578–586.

    PubMed  CAS  Google Scholar 

  • Berthier, R., Marin, M., Duperray, A., Prenant, M., Newton, I., Schweitzer, A., Hollard, D., and Marguerie, G., 1987, Megakaryocytic development in liquid cultures of cryopreserved leukocyte stem cell concentrates from chronic myelogenous leukemia patients, Exp. Hematol. 15: 750–758.

    PubMed  CAS  Google Scholar 

  • Berthier, R., Valiron, O., Troesch, A., Clemancey-Marcille, G., Schweitzer, A., and Hollard, D., 1989, Cryopreservation of human megakaryocytic progenitor cells (CFU-MK): Influence of culture conditions, Cryobiology 26: 265–272.

    PubMed  CAS  Google Scholar 

  • Bonfanti, R., Furie, B. C., Furie, B., and Wagner, D., 1989, PADGEM (GMP140) is a component of WeibelPalade bodies of human endothelial cells, Blood 73: 1109–1112.

    PubMed  CAS  Google Scholar 

  • Bonthron, D., On, E. C., Mitsock, L. M., Ginsburg, D., Handin, R. I., and Orkin, S. H., 1986, Nucleotide sequence of pre-pro-von Willebrand factor cDNA, Nucleic Acids Res. 14:.7125–7127.

    Google Scholar 

  • Bray, P. F., Rosa, J. P., Lingappa, V. R., Kan, Y. W., McEver, R. P., and Shuman, M. A., 1986, Biogenesis of the platelet receptor for fibrinogen: Evidence for separate precursors for glycoproteins IIb and IIIa, Proc. Natl. Acad. Sci. USA 83: 1480–1484.

    PubMed  CAS  Google Scholar 

  • Bray, P. F., Rosa, J. P., Johnston, G., Shiu, D. T., Cook, R. G., Lau, C., Kan, Y. W., McEver, R. P., and Shuman, M. A., 1987, Platelet glycoprotein IIb. Chromosomal localization and tissue expression, J. Clin. Invest. 80: 1812–1817.

    PubMed  CAS  Google Scholar 

  • Burgess, T. L., and Kelly, R. B., 1987, Constitutive and regulated secretion of proteins, Annu. Rev. Cell Biol. 3: 243–293.

    PubMed  CAS  Google Scholar 

  • Cerveny, T. J., Fass, D. N., and Mann, K. G., 1984, Synthesis of coagulation factor V by cultured aortic endothelium, Blood 63: 1467–1474.

    PubMed  CAS  Google Scholar 

  • Chesney, C. M., Pifer, D., and Colman, R. W., 1981, Subcellular localization and secretion of factor V from human platelets, Proc. Natl. Acad. Sci. USA 78: 5180–5184.

    PubMed  CAS  Google Scholar 

  • Chesterman, C. N., and Berndt, M. C., 1986, Platelet and vessel wall interaction and the genesis of atherosclerosis, Clin. Haematol. 15: 323–353.

    PubMed  CAS  Google Scholar 

  • Chiu, H. C., Schick, P. K., and Colman, R. W., 1985, Biosynthesis of factor V in isolated guinea pig megakaryocytes, J. Clin. Invest. 75: 339–346.

    PubMed  CAS  Google Scholar 

  • Coller, B. S., Peerschke, E. I., and Sullivan, C. A., 1983, Studies with a murine monoclonal antibody that abolishes risocetin-induced binding of von Willebrand factor to platelets: Additional evidence in support of GPIb as a platelet receptor for von Willebrand factor, Blood 61: 99–110.

    PubMed  CAS  Google Scholar 

  • Comp, P. C., Nixon, R. R., Cooper, M. R., and Esmon, C. T., 1984, Familial protein S deficiency is associated with recurrent thrombosis, J. Clin. Invest. 74: 2082–2088.

    PubMed  CAS  Google Scholar 

  • Crabtree, G. R., and Kant, J. A., 1982, Organization of the rat T-fibrinogen gene: Alternative mRNA splice patterns produce the TA and TB(T’) chains of fibrinogen, Cell 31: 159–166.

    PubMed  CAS  Google Scholar 

  • Cramer, E. M., Vainchenker, W., Vinci, G., Guichard, J., and Breton-Gorius, J., 1985, Gray platelet syndrome: Immunoelectron microscopic localization of fibrinogen and von Willebrand factor in platelets and megakaryocytes, Blood 66: 1309–1316.

    PubMed  CAS  Google Scholar 

  • Cramer, E. M., Debili, N., Martin, J. F., Gladwin, A. M., Breton-Gorius, J., Harrison, P., Savidge, G. F., and Vainchenker, W., 1989, Uncoordinated expression of fibrinogen compared to thrombospondin and von Willebrand factor in maturing human megakaryocytes, Blood 73: 1123–1129.

    PubMed  CAS  Google Scholar 

  • Damiani, G., Zocchi, E., Fabbi, M., Bargellesi, A., and Patrone, F., 1983, A monoclonal antibody to platelet glycoproteins IIb and IIIa complex: Its use in purifying human megakaryocytes from sternal bone marrow aspirates for immunofluorescence studies of la-like antigens, Exp. Hematol. 11: 169–177.

    PubMed  CAS  Google Scholar 

  • De Alarcon, P. A., 1989, Megakaryocyte colony-stimulating factor (Mk-CSF): Its physiologic significance, Blood Cells 15: 173–184.

    PubMed  Google Scholar 

  • Dessypris, E. N., Gleaton, J. H., and Armstrong, O. L., 1987, Effect of human recombinant erythropoietin on human marrow megakaryocyte colony formation in vitro. Br. J. Haematol. 65: 265–269.

    PubMed  CAS  Google Scholar 

  • Duperray, A., Berthier, R., Chagnon, E., Ryckewaert, J. J., Ginsberg, M., Plow, E., and Marguerie, G., 1987, Biosynthesis and processing of platelet GPIIb-IIIa in human megakaryocytes, J. Cell Biol. 104: 1665 1673.

    Google Scholar 

  • Duperray, A., Troesch, A., Berthier, R., Chagnon, E., Frachet, P., Uzan, G., and Marguerie, G., 1989, Biosynthesis and assembly of platelet GPIIb-IIIa in human megakaryocytes: Evidence that assembly between pro-GPIIb and GPIIIa is a prerequisite for expression of the complex on the cell surface, Blood 74: 1603–1611.

    PubMed  CAS  Google Scholar 

  • Fair, D. S., and Marlar, R. A., 1986, Biosynthesis and secretion of factor VII, protein C, protein S, and the protein C inhibitor from a human hepatoma cell line, Blood 67: 64–70.

    PubMed  CAS  Google Scholar 

  • Fair, D. S., Marlar, R. A., and Levin, E. G., 1986, Human endothelial cells synthesize protein S, Blood 67: 1168–1171.

    PubMed  CAS  Google Scholar 

  • Fitzgerald, L. A., Steiner, B., Rall, S. C., Lo, S., and Phillips, D. R., 1987, Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to “Integrin,” J. Biol. Chem. 262: 3936–3939.

    PubMed  CAS  Google Scholar 

  • Fox, J. E. B., 1985, Identification of actin-binding protein as the protein linking the membrane skeleton to glycoproteins on platelet plasma membranes, J. Biol. Chem. 260: 11970–11977.

    PubMed  CAS  Google Scholar 

  • Fraser, J. K., Tan, A. S., Lin, F. K., and Berridge, M. V., 1989, Expression of high-affinity binding sites for erythropoietin on rat and mouse megakaryocytes, Exp. Hematol. 17: 10–16.

    PubMed  CAS  Google Scholar 

  • Gewirtz, A. M., Bruno, E., Elwell, J., and Hoffman, R., 1983, In vitro studies of megakaryocytopoiesis in thrombocytotic disorders of man, Blood 61: 384–389.

    PubMed  CAS  Google Scholar 

  • Gewirtz, A. M., Keefer, M., Doshi, K., Annamalai, A. E., Chiu, H. C., and Colman, R. W., 1986, Biology of human megakaryocyte factor V, Blood 67: 1639–1648.

    PubMed  CAS  Google Scholar 

  • Ginsberg, M. H., Loftus, J. C., Ryckewaert, J. J., Pierschbacher, M., Pytela, R., Ruoslathi, E., and Plow, E. F., 1987, Immunochemical and amino-terminal sequence comparison of two cytoadhesins indicates they contain similar or identical beta subunits and distinct alpha subunits, J. Biol. Chem. 262: 5437–5440.

    PubMed  CAS  Google Scholar 

  • Grant, B. W., Nichols, W. L., Solberg, L. A., Yachimiak, D. J., and Mann, K. G., 1986, A radioimmunoassay useful for quantitating megakaryocyte growth in vitro, in Megakaryocyte Development and Function ( R. F. Levine, N. Williams, J. Levine, and B. L. Evatt, eds.), pp. 117–121, Alan R. Liss, Inc., New York.

    Google Scholar 

  • Greenberg, S. M., Rosenthal, D. S., Greeley, T. A., Tantravahi, R., and Handin, R. I., 1988, Characterization of a new megakaryocytic cell line: The Dami cell, Blood 72: 1968–1977.

    PubMed  CAS  Google Scholar 

  • Handagama, P. J., George, J. N., Shuman, M. A., McEver, R. P., and Bainton, D. F., 1987, Incorporation of a circulating protein into megakaryocyte and platelet granules, Proc. Natl. Acad. Sci. USA 84: 861–865.

    PubMed  CAS  Google Scholar 

  • Harmon, J. T., and Jamieson, G. A., 1986, Activation of platelets by a-thrombin is a receptor-mediated event, J. Biol. Chem. 261: 15928–15933.

    PubMed  CAS  Google Scholar 

  • Henry, I., Uzan, G., Weil, D., Nicolas, H., Kaplan. J. C., Marguerie, G., Kahn, A., and Julien, C., 1984, The genes coding for An- Bß-, and -r-chains of fibrinogen map to 4q2, Am. J. Hum. Genet. 36: 760–768.

    CAS  Google Scholar 

  • Hibbin, J. A., Matutes, E., and Goldman, J. M., 1985, Proliferation in liquid culture of megakaryocytes from the blood of patients with primary myelofibrosis and other myeloproliferative disorders, Leuk. Res. 9: 1171–1179.

    PubMed  CAS  Google Scholar 

  • Hoeprich, P., and Doolittle, R. F., 1983, Dimeric half-molecules of human fibrinogen are joined through disulfide bonds in an antiparallel orientation, Biochemistry 22: 2049–2055.

    PubMed  CAS  Google Scholar 

  • Hourdillé, P., Fialon, P., Belloc, F., Namur, M., Boisseau, M. R., and Nurden, A. T., 1986, Megakaryocytes from the marrow of a patient with Glansmann’s thrombasthenia lacked GP IIb-IIIa complexes, Thromb. Haemostasis 56: 66–70.

    Google Scholar 

  • Hsu-Lin, S. C., Berman, C. L., Furie, B. C., August, D., and Furie, B., 1984, A platelet membrane protein expressed during platelet activation and secretion; studies using a monoclonal antibody specific for thrombin-activated platelets, J. Biol. Chem. 259: 9121–9126.

    PubMed  CAS  Google Scholar 

  • Ishibashi, T., and Burstein, S. A., 1985, Separation of murine megakaryocytes and their progenitors on continuous gradients of percoll, J. Cell. Physiol. 125: 559–566.

    PubMed  CAS  Google Scholar 

  • Ishibashi, T., Ruggeri, Z. M., Harker, L. A., and Burstein, S. A., 1986, Separation of human megakaryocytes by state of differentiation on continuous gradients of percoli: Size and ploidy analysis of cells identified by monoclonal antibody to glycoprotein IIb/IIIa, Blood 67: 1286–1292.

    PubMed  CAS  Google Scholar 

  • Ishibashi, T., Koziol, J. A., and Burstein, S. A., 1987, Human recombinant erythropoietin promotes differentiation of murine megakaryocytes in vitro, J. Clin. Invest. 79: 286–289.

    PubMed  CAS  Google Scholar 

  • Jackson, C. W., Brown, L. K., Somerville, B. C., Lyles, S. A., and Look, A. T., 1984, Two-color flow cytometric measurement of DNA distributions of rat megakarocytes in unfixed, unfractionated marrow cell suspensions, Blood 63: 768–778.

    PubMed  CAS  Google Scholar 

  • Jackson, C. W., Steward, S. A., Brown, L. K., and Look, A. T., 1986, Inverse relationship between megakaryocyte buoyant density and maturity, Br. J. Haematol. 64: 33–43.

    PubMed  CAS  Google Scholar 

  • Jackson, C. W., Steward, S. A., Hutson, N. K., and Ashmun, R. A., 1987, Interaction of ristocetin and bovine plasma with guinea pig megakaryocytes: A means to enrich megakaryocytes based on membrane rather than physical characteristics, Blood 69: 173–179.

    PubMed  CAS  Google Scholar 

  • Jaffe, E. A., Hoyer, L. W., and Nachman, R. L., 1973, Synthesis of antihemophilic factor antigen by cultured human endothelial cells, J. Clin. Invest. 52: 2757–2764.

    PubMed  CAS  Google Scholar 

  • Jandrot-Perrus, M., Mosesson, M. W., Denniger, M. H., and Ménaché, D., 1979, Studies of platelet fibrinogen from a subject with a congenital plasma fibrinogen abnormality (fibrinogen Paris I), Blood 54: 1109–1116.

    PubMed  CAS  Google Scholar 

  • Jenkins, R. B., Nichols, W. L., Mann, K. G., and Solberg, L. A., 1986, CFU-M-derived human mega-karyocytes synthesize glycoproteins IIb and IIIa, Blood 67: 682–688.

    PubMed  CAS  Google Scholar 

  • Jennings, L. K., and Phillips, D. R., 1982, Purification of glycoproteins IIb and III from human platelet plasma membranes and characterization of a calcium-dependent glycoprotein IIh-III complex, J. Biol. Chem. 257: 10458–10466.

    PubMed  CAS  Google Scholar 

  • Jenny, R. J., Pittman, D. D., Toole, J. J., Kriz, R. W., Aldape, R. A., Hewick, R. M., Kaufman, R. J., and Mann, K. G., 1987, Complete cDNA and derived amino acid sequence of human factor V, Proc. Natl. Acad. Sci. USA 84: 4846–4850.

    PubMed  CAS  Google Scholar 

  • Johnston, G. I., Cook, R. G., and McEver, R. P., 1989a, Cloning of GMP-140, a granule membrane protein of platelets and endothelium: Sequence similarity to proteins involved in cell adhesion and inflammation, Cell 56: 1033–1044.

    PubMed  CAS  Google Scholar 

  • Johnston, G. I., Kurosky, A., and McEver, R. P., 1989b, Structural and biosynthetic studies of the granule membrane protein, GMP-140, from human platelets and endothelial cells, J. Biol. Chem. 264: 1816–1823.

    PubMed  CAS  Google Scholar 

  • Judson, P. A., Anstee, D., and Clamp, J. R., 1982, Isolation and characterization of the major oligosaccharide of human platelet membrane glycoprotein GPIb, Biochem. J. 205: 81–90.

    PubMed  CAS  Google Scholar 

  • Juvonen, E., 1988, Megakaryocyte colony formation in chronic myeloid leukemia and myelofibrosis, Leuk. Res. 12: 751–756.

    PubMed  CAS  Google Scholar 

  • Kajigaya, S., Suda, T., Suda, J., Eguchi, M., Moroi, M., Sudo, T., Saito, M., and Miura, Y., 1988, Establishment of megakaryoblastic cell lines by coinfection of Abelson murine leukemia virus and recombinant SV40-retrovirus, Jpn. J. Cancer Res. 79: 726–733.

    PubMed  CAS  Google Scholar 

  • Kane, W., and Davie, E. W., 1986, Cloning of a cDNA coding for human factor V, a blood coagulation factor homologous to factor VII and ceruloplasmin, Proc. Natl. Acad. Sci. USA 83: 6800–6804.

    PubMed  CAS  Google Scholar 

  • Kane, W. H., Lindhout, M. J., Jackson, C. M., and Majerus, P. W., 1980, Factor Va-dependent binding of factor Xa to human platelets, J. Biol. Chem. 255: 1170–1174.

    PubMed  CAS  Google Scholar 

  • Kane, W. H., Ichinose, A., Hagen, F. S., and Davie, E. W., 1987, Cloning of cDNAs coding for the heavy chain region and connecting region of human factor V, a blood coagulation factor with four types of internal repeats, Biochemistry 26: 6508–6514.

    PubMed  CAS  Google Scholar 

  • Kant, J. A., Fornace, A. J., Saxe, D., Simon, M. I., McBride, O. W., and Crabtree, G. R., 1985, Evolution and organization of the fibrinogen locus on chromosome 4: Gene duplication accompanied by transposition and inversion, Proc. Natl. Acad. Sci. USA 82: 2344–2348.

    PubMed  CAS  Google Scholar 

  • Kaplan, K. L., Broekman, M. J., Chernoff, A., Lesznik, G. R., and Drillings, M., 1979, Platelet a-granules proteins: Studies on release and subcellular localization, Blood 53: 604–618.

    PubMed  CAS  Google Scholar 

  • Kieffer, N., Debili, N., Wield, A., Titeux, M., Henri, A., Mishal, Z., Breton-Gorius, J., Vainchenker, W., and Clemetson, K. J., 1986, Expression of platelet glycoprotein Iba in HEL cells, J. Biol. Chem. 261: 15854–15862.

    PubMed  CAS  Google Scholar 

  • Kieffer, N., Guichard, J., Farcet, J. P., Vainchenker, and Breton-Gorius, J., 1987, Biosynthesis of major platelet proteins in human blood platelets, Eur. J. Biochem. 164: 189–195.

    PubMed  CAS  Google Scholar 

  • Kieffer, N., Wautier, J. L., Coulombel, L., Titeux, M., Wautier, M. P., Vainchenker, W., Ruan, C., and BretonGorius, J., 1988, Uncoupling in the expression of platelet GPIIb/IIIa in human endothelial cells and K562 cells: Absence of immunologic crossreactivity between platelet GPIIb and the vitronectin receptor alpha chain, Blood 72: 1209–1215.

    PubMed  CAS  Google Scholar 

  • Korrel, S. A. M., Clemetson, K. J., Van Halbeek, H., Kamerling, J. P., Sixma, J. J., and Vliegenthart, F. G., 1984, Structural studies on the 0-linked carbohydrate chains of human platelet glycocalicin, Eur. J. Biochem. 140: 571–576.

    PubMed  CAS  Google Scholar 

  • Legaz, M. E., Schmer, G., Counts, R. B., and Davie, E. W., 1973, Isolation and characterization of human factor VIII (antihemophilic factor), J. Biol. Chem. 248: 3946–3955.

    PubMed  CAS  Google Scholar 

  • Leven, R. M., Schick, P. K., and Budzynski, A. Z., 1985, Fibrinogen biosynthesis in isolated guinea pig megakaryocytes, Blood 65: 501–504.

    PubMed  CAS  Google Scholar 

  • Levine, R. F., and Fedorko, M. F., 1976, Isolation of intact megakaryocytes from guinea pig femoral marrow, J. Cell Biol. 69: 159–172.

    PubMed  CAS  Google Scholar 

  • Lingappa, V. R., 1989, Intracellular traffic of newly synthesized proteins. Current understanding and future prospects, J. Clin. Invest. 83: 739–751.

    PubMed  CAS  Google Scholar 

  • Lopez, J. A., Chung, D. W., Fujikawa, K., Hagen, F. S., Papayannopoulou, T., and Roth, G. J., 1987, Cloning of the a chain of human platelet glycoprotein Ib: A transmembrane protein with homology to leucine-rich a2-glycoprotein, Proc. Natl. Acad. Sci. USA 84: 5615–5619.

    PubMed  CAS  Google Scholar 

  • Lopez, J. A., Chung, D. W., Fujikawa, K., Hagen, F. S., Davie, E. W., and Roth, G. J., 1988, The a and 13 chains of human platelet glycoprotein Ib are both transmembrane proteins containing a leucine-rich amino acid sequence, Proc. Natl. Acad. Sci. USA 85: 2135–2139.

    PubMed  CAS  Google Scholar 

  • Lozzio, C. B., and Lozzio, B. B., 1975, Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome, Blood 45: 321–334.

    PubMed  CAS  Google Scholar 

  • Lundwall, A., Dackowski, W., Cohen, E., Shaffer, M., Mahr, A., Dahlback, B., Stenflo, J., and Wydro, R., 1986, Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation, Proc. Natl. Acad. Sci. USA 83: 6716–6720.

    PubMed  CAS  Google Scholar 

  • Marguerie, G. A., Plow, E. F., and Edgington, T. S., 1979, Human platelets possess an inducible and saturable receptor specific for fibrinogen, J. Biol. Chem. 254: 5357–5363.

    PubMed  CAS  Google Scholar 

  • Marguerie, G., Ginsberg, M. H., and Plow, E. F., 1987, The platelet fibrinogen receptor, in Platelets in Biology and Pathology Ill ( D. E. Macintyre and J. L. Gordon, eds.), pp. 95–125, Elsevier, New York.

    Google Scholar 

  • Martin, P., and Papayannopoulou, T., 1982, HEL cells: A new human erythroleukemia cell line with spontaneous and induced globin expression, Science 216: 1233–1235.

    PubMed  CAS  Google Scholar 

  • Martin, S. E., Marder, V. J., Francis, C. W., and Barlow, G. H., 1981, Structural studies on the functional heterogeneity of von Willebrand protein polymers, Blood 57: 313–323.

    PubMed  CAS  Google Scholar 

  • McEver, R. P., and Martin, M. N., 1984, A monoclonal antibody to a membrane glycoprotein binds only to activated platelets, J. Biol. Chem. 259: 9799–9804.

    PubMed  CAS  Google Scholar 

  • McLeod, D. L., Shreeve, M. M., and Axelrad, A. A., 1980, Chromosome marker evidence for the bipotentiality of BFU-E, Blood 56: 318–322.

    PubMed  CAS  Google Scholar 

  • Messner, H. A., Jamal, N., and Izaguirre, C., 1982, The growth of large megakaryocyte colonies from human bone marrow, J. Cell. Physiol. Suppl. 1: 45–51.

    PubMed  CAS  Google Scholar 

  • Miletich, J. P., Jackson, C. M., and Majerus, P. W., 1978, Properties of the factor Xa binding site on human platelets, J. Biol. Chem. 253: 6908–6916.

    PubMed  CAS  Google Scholar 

  • Mosesson, M. W., Homandberg, G. A., and Amrani, D. L., 1984, Human platelet fibrinogen gamma chain structure, Blood 63: 990–995.

    PubMed  CAS  Google Scholar 

  • Nachman, R., Levine, R., and Jaffe, E. A., 1977, Synthesis of factor VIII antigen by cultured guinea pig megakaryocytes, J. Clin. Invest. 60: 914–921.

    PubMed  CAS  Google Scholar 

  • Nakeff, A., Valeriote, F., Gray, J. W., and Grabske, R. J., 1979, Application of flow cytometry and cell sorting to megakaryocytopoiesis, Blood 53: 732–745.

    PubMed  CAS  Google Scholar 

  • Nurden, A. T., 1987, Platelet membrane glycoproteins and their clinical aspects, in Thrombosis and Haemostat-sis ( M. Verstraete, J. Vermylen, H. R., Lijnen, and J. Arnout, eds.), pp. 93–125, International Society on Thrombosis and Haemostasis and Leuven Press, Leuven, Belgium.

    Google Scholar 

  • Ogura, M., Morishima, Y., Ohno, R., Kato, Y., Hirabayashi, N., Nagura, H., and Saito, H., 1985, Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome, Blood 66: 1384–1392.

    PubMed  CAS  Google Scholar 

  • Ogura, M., Tanabe, N., Nishioka, J., Suzuki, K., and Saito, H., 1987, Biosynthesis and secretion of functional protein S by a human megakaryoblastic cell line (MEG-01), Blood 70: 301–306.

    PubMed  CAS  Google Scholar 

  • Okumura, T., and Jamieson, G. A., 1976, Platelet glycocalicin. I. Orientation of glycoproteins of the human platelet surface, J. Biol. Chem. 251: 5944–5949.

    PubMed  CAS  Google Scholar 

  • Okumura, T., Lombart, C., and Jamieson, G. A., 1976, Platelet glycocalcin. II. Purification and characterization, J. Biol. Chem. 251: 5950–5955.

    PubMed  CAS  Google Scholar 

  • Okumura, T., Hasitz, M., and Jamieson, G. A., 1978, Platelet glycocalicin. Interaction with thrombin and role as thrombin receptor of the platelet surface, J. Biol. Chem. 253: 3435–3443.

    PubMed  CAS  Google Scholar 

  • Papayannopoulou, T., Raines, E., Collins, S., Nakamoto, B., Tweeddale, M., and Ross, R., 1987, Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers, J. Clin. Invest. 79: 859–866.

    PubMed  CAS  Google Scholar 

  • Phillips, D. R., and Agin, P. P., 1977a, Platelet plasma membrane glycoproteins. Evidence for the presence of nonequivalent disulfide bonds using nonreduced-reduced two-dimensional gel electrophoresis, J. Biol. Chem. 252: 2121–2126.

    PubMed  CAS  Google Scholar 

  • Phillips, D. R., and Agin, P. P., 1977b, Platelet membrane defects in Glanzmann’s thrombasthenia: Evidence for decreased amounts of two major glycoproteins, J. Clin. Invest. 60: 535–545.

    PubMed  CAS  Google Scholar 

  • Phillips, D. R., and Jakabova, M., 1977, Cat+-dependent protease in human platelets. Specific cleavage of platelet polypeptides in the presence of added Cat+, J. Biol. Chem. 252: 5602–5605.

    PubMed  CAS  Google Scholar 

  • Phillips, D. R., Charo, I. F., Panse, L. V., and Fitzgerald, L. A., 1988, The platelet membrane glycoprotein IIb-IIIa complex, Blood 71: 831–843.

    PubMed  CAS  Google Scholar 

  • Plow, E. F., and Ginsberg, M., 1981, Specific and saturable binding of plasma fibronectin to thrombin stimulated human platelet, J. Biol. Chem. 256: 9477–9482.

    PubMed  CAS  Google Scholar 

  • Plow, E. F., Loftus, J. C., Levin, E. G., Fair, D. S., Dixon, S., Forsyth, J., and Ginsberg, M. H., 1986, Immunological relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells, Proc. Natl. Acad. Sci. USA 83: 6002–6006.

    PubMed  CAS  Google Scholar 

  • Poncz, M., Eisman, R., Heidenreich, R., Silver, S. M., Vilaire, G., Surrey, S., Schartz, E., and Bennett, J. S., 1987, Structure of the platelet membrane glycoprotein IIb: Homology to the a subunits of the vitronectin and fibronectin membrane receptors, J. Biol. Chem. 262: 8476–8482.

    PubMed  CAS  Google Scholar 

  • Rabellino, E. M., Nachman, R. L., Williams, N., Winchester, R. J., and Ross, G. D., 1979, Human megakaryocytes. I. Characterization of the membrane and cytoplasmic components of isolated marrow megakaryocytes, J. Exp. Med. 149: 1273–1287.

    PubMed  CAS  Google Scholar 

  • Rosa, J. P., George, J. N., Bainton, D. F., Nurden, A. T., Caen, J. P., and McEver, R. P., 1987, Gray platelet syndrome. Demonstration of alpha granule membranes that can fuse with the cell surface, J. Cin. Invest. 80: 1138–1146.

    CAS  Google Scholar 

  • Rosa, J. P., Bray, P. F., Gayet, O., Johnston, G. I., Cook, R. G., Jackson, K. W., Shuman, M. A., and McEver, R. P., 1988, Cloning of glycoprotein IIIa cDNA from human erythroleukemia cells and localization of the gene to chromosome 17, Blood 72: 593–600.

    PubMed  CAS  Google Scholar 

  • Roth, B. J., Sledge, G. W., Straneva, J. E., Brandt, J., Goheen, M., and Hoffman, R., 1988, Analysis of phorbol ester stimulated human megakaryocyte development, Blood 72: 202–207.

    PubMed  CAS  Google Scholar 

  • Ruggeri, Z. M., Bader, R., and De Marco, L., 1982, Glanzmann thrombastenia: Deficient binding of von Willebrand factor to thrombin-stimulated platelets, Proc. Natl. Acad. Sci. USA 79: 6038–6041.

    PubMed  CAS  Google Scholar 

  • Sakariassen, K. S., Bolhuis, P. A., and Sixma, J. J., 1979, Human blood platelet adhesion to artery subendothelium is mediated by factor VII-von Willebrand factor bound to subendothelium, Nature (London) 279: 636–638.

    CAS  Google Scholar 

  • Sanderson, R. J., and Bird, K., 1977, Cell separation by counterflow centrifugation, Methods Cell. Biol. 15: 114.

    Google Scholar 

  • San Miguel, J. F., Tavares de Castro, J., Matutes, E., Rodriguez, B., Polli, N., Zola, H., McMichael, A. J., Bollum, F. J., Thompson, D. S., Goldman, J. M., and Catovsky, D., 1985, Characterization of blast cells in chronic granulocytic leukaemia in transformation, acute myelofibrosis and undifferentiated leukaemia, Br. J. Haematol. 59: 297–309.

    PubMed  CAS  Google Scholar 

  • Schwarz, H. P., Heeb, M. J., Wencel-Drake, J. D., and Griffin, J. H., 1985, Identification and quantification of protein S in human platelets, Blood 66: 1452–1455.

    PubMed  CAS  Google Scholar 

  • Seigneurin, D., Champelovier, P., Mouchiroud, G., Berthier, R., Leroux, D., Prenant, M., McGregor, J., Starck, J., Morle, F., Micouin, C., Pietrantuono, A., and Kolodie, L., 1987, Human chronic myeloid leukemic cell line with positive Philadelphia chromosome exhibits megakaryocytic and erythroid characteristics, Exp. Hematol. 15: 822–832.

    PubMed  CAS  Google Scholar 

  • Shelton-Inloes, B., Titani, K., and Sadler, J. E., 1986, cDNA sequences for human von Willebrand factor reveal five types of repeated domains and five possible protein sequence polymorphisms, Biochemistry 25:3164–3171.

    Google Scholar 

  • Shoff, P. K., and Levine, R. F., 1989, Elutriation for isolation of megakaryocytes, Blood Cells 15: 285–305.

    PubMed  CAS  Google Scholar 

  • Silver, S. M., McDonough, M. M., Vilaire, G., and Bennett, J. S., 1987, The in vitro synthesis of polypeptides for the platelet membrane glycoproteins IIb and IIIa, Blood 69: 1031–1037.

    PubMed  CAS  Google Scholar 

  • Sitar, G., 1984, Isolation of normal human megakaryocytes, Br. J. Haematol. 58: 465–472.

    PubMed  CAS  Google Scholar 

  • Sitar, G., Borroni, R., and Pesce, G., 1986, Synthesis of thrombospondin and glycoprotein IIb-IIIa by a purified megakaryocyte human bone marrow culture, Haematologica 71: 389–393.

    PubMed  CAS  Google Scholar 

  • Sledge, G. W., Giant, M., Jansen, J., Heerema, N. A., Roth, B. J., Goheen, M., and Hoffman, R., 1986, Establishment in long term culture of megakaryocytic leukemia cells (EST-IU) from the marrow of a patient with leukemia and a mediastinal germ cell neoplasm, Cancer Res. 46: 2155–2159.

    PubMed  Google Scholar 

  • Solberg, L. A., Jamal, N., and Messner, H. A., 1985, Characterization of human megakaryocytic colony formation in human plasma, J. Cell. Physiol. 124: 67–74.

    PubMed  Google Scholar 

  • Soria, J., Soria, C., Samama, M., Henschen, A., and Southan, C., 1982, Detection of fibrinogen abnormality in dysfibrinogenemia. Special report on fibrinogen Metz characterized by an amino acid substitution located at the peptide bond cleaved by thrombin, in Fibrinogen. Recent Biomedical Aspects, ( A. Henshen, H. Graeff, and F. Lottspeich, eds.), pp. 126–143, W. de Gruyter and Co., New York.

    Google Scholar 

  • Sporn, L. A., Chavin, S. I., Marder, V. J., and Wagner, D. D., 1985, Biosynthesis of von Willebrand protein by human megakaryocytes, J. Clin. Invest. 76: 1102–1106.

    PubMed  CAS  Google Scholar 

  • Stenberg, P. E., McEver, R. P., Shuman, M. A., Jacques, Y. V., and Bainton, D., 1985, A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation, J. Cell Biol. 101: 880–886.

    PubMed  CAS  Google Scholar 

  • Stern, D., Brett, J., Harris, K., and Nawroth, P., 1986, Participation of endothelial cells in the protein C-protein S anticoagulant pathway: The synthesis and release of protein S, J. Cell Biol. 102: 1971–1978.

    PubMed  CAS  Google Scholar 

  • Suzuki, S., Argraves, W. S., Arai, H., Languino, L. R., Pierschbacher, M. D., and Ruoslathi, E., 1987, Amino-acid sequence of the vitronectin receptor a-subunit and comparative expression of adhesion receptor mRNAs, J. Biol. Chem. 262: 14080–14084.

    PubMed  CAS  Google Scholar 

  • Tange, T., Nakahara, K., Mitani, K., Yamasaki, I., Yasuda, H., Tanaka, F., Mizuguchi, M., Oda, H., Yatomi, Y., Takanashi, R., Fujioka, S., Yamaguchi, K., and Urano, Y., 1988, Establishment of a human megakaryoblastic cell line (T-33) from chronic myelogenous leukemia in megakaryoblastic crisis, Cancer Res. 48: 6137–6144.

    PubMed  CAS  Google Scholar 

  • Tarentino, A. L., and Maley, F., 1974, Purification and properties of an endo-3-N-acetylglucosaminidase from streptomyces griseus, J. Biol. Chem. 249: 811–817.

    PubMed  CAS  Google Scholar 

  • Tartakoff, A. M., 1983, Perturbation of vesicular traffic with carboxylic ionophore monensin, Cell 32: 1026–1028.

    PubMed  CAS  Google Scholar 

  • Titani, K., Kumar, S., Takio, K., Ericsson, L. H., Wade, R. D., Ashida, K., Walsh, K. A., Chopek, M. W., Sadler, J. E., and Fujikawa, K., 1986, Amino acid sequence of human von Willebrand factor, Biochemistry 25: 3171–3184.

    PubMed  CAS  Google Scholar 

  • Titani, K., Takio, K., Banda, M., and Ruggeri, Z. M., 1987, Amino acid sequence of the von Willebrand factor-binding domain of platelet membrane glycoprotein Ib, Proc. Natl. Acad. Sci. USA 84: 5610–5614.

    PubMed  CAS  Google Scholar 

  • Tomer, A., Harker, L. A., and Burstein, S. A., 1987, Purification of human megakaryocytes by fluorescenceactivated cell sorting, Blood 70: 1735–1742.

    PubMed  CAS  Google Scholar 

  • Townsend, R. R., Hilliker, E., Li, Y. T., Laine, R. A., Bell, W. R., and Lee, Y. C., 1982, Carbohydrate structure of human fibrinogen. Use of 300 H-NMR to characterize glycosidase-treated glycopeptides, J. Biol. Chem. 257: 9704–9710.

    PubMed  CAS  Google Scholar 

  • Tsuji, T., Tsunehisa, S., Watanabe, Y., Yamamoto, K., Tohyama, H., and Osawa, T., 1983, The carbohydrate moiety of human platelet glycocalicin. The structure of the major Ser/Thr-linked sugar chain, J. Biol. Chem. 258: 6335–6339.

    PubMed  CAS  Google Scholar 

  • Uzan, G., Courtois, G., Stanckovic, Z., Crabtree, G. R., and Marguerie, G., 1986, Expression of the fibrinogen genes in rat megakaryocytes, Biochem. Biophys. Res. Commun. 140: 543–549.

    PubMed  CAS  Google Scholar 

  • Uzan, G., Frachet, P., Lajmanovich, A., Prandini, M. H., Denarier, E., Duperray, A., Loftus, J., Ginsberg, M., Plow, E., and Marguerie, G., 1988, cDNA clones for human paltelet GPIIIb corresponding to mRNA of megakaryocytes and erythroleukemic cells: Evidence for an extensive homology to other Arg-Gly-Asp adhesion receptors, Eur. J. Biochem. 171: 87–93.

    Google Scholar 

  • Valiron, O., Clémancey-Marcille, G., Troesch, A., Schweitzer, A., Prenant, M., Bollard, D., and Berthier, R., 1988, Immunophenotype of blast cells in chronic myeloid leukemia, Leuk. Res. 12: 861–872.

    PubMed  CAS  Google Scholar 

  • Verweij, C. L., Diergaarde, P. J., Hart, M., and Pannekoek, H., 1986, Full-length von Willebrand factor (vWF) cDNA encodes a highly repetitive protein considerably larger than the mature vWF subunit, EMBO J. 5: 1839–1847.

    PubMed  CAS  Google Scholar 

  • Vicic, W., Lages, B., and Weiss, H., 1980, Release of human platelet factor V activity is induced by both collagen and ADP and is inhibited by aspirin, Blood 56: 448–455.

    PubMed  CAS  Google Scholar 

  • Vinci, G., Tabilio, A., Deschamps, J. F., Van Haeke, D., Henri, A., Guichard, J., Tetteroo, P., and Landsorp, P. M., Hercend, T., Vainchenker, W., and Breton-Gorius, J., 1984, Immunological study of in vitro maturation of human megakaryocytes, Br. J. Haematol. 58: 589–605.

    Google Scholar 

  • Wagner, D. D., and Marder, V. J., 1984, Biosynthesis of von Willebrand protein by human endothelial cells: Processing steps and their intracellular localization, J. Cell Biol. 99: 2123–2130.

    PubMed  CAS  Google Scholar 

  • Wagner, D. D., Olmsted, J. B., and Marder, V. J., 1982, Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells, J. Cell Biol. 95: 355–360.

    PubMed  CAS  Google Scholar 

  • Walker, F. J., 1980, Regulation of activated protein C by a new protein. A possible function for protein S, J. Biol. Chem. 255: 5521–5524.

    PubMed  CAS  Google Scholar 

  • Weinstein, R., Stemerman, M. B., Maclntyre, D. E., Steinberg, H. N., and Maciag, T., 1981, The morphological and biochemical characterization of a line of rat promegakaryoblasts, Blood 58: 110–121.

    PubMed  CAS  Google Scholar 

  • Wilson, D. B., Salem, H. H., Mruk, J. S., Maruyama, I., and Majerus, P. W., 1983, Biosynthesis of coagulation factor V by a human hepatocellular carcinoma cell line, J. Clin. Invest. 73: 654–658.

    Google Scholar 

  • Worthington, R. E., and Nakeff, A., 1981, Thromboxane synthesis in megakaryocytes isolated by centrifugal elutriation, Blood 58: 175–178.

    PubMed  CAS  Google Scholar 

  • Wyler, B., Bienz, D., Clemetson, K. J., and Luscher, E. F., 1986, Glycoprotein Ibß is the only phosphorylated major membrane glycoprotein in human platelets, Biochem. J. 234: 373–379.

    PubMed  CAS  Google Scholar 

  • Yeo, E., Furie, B., and Furie, B., 1989, PADGEM protein in human erythroleukemia cells, Blood 73:722–728. Zimmerman, T. S., Ruggeri, Z. M., and Fulcher, C. A., 1983, Factor VIII/von Willebrand factor, in Progress in Hematology ( E. B. Brown, ed.), Vol. 3, pp. 279–309, Grune and Stratton, New York.

    Google Scholar 

  • Zimrin, A. B., Eisman, R., Vilaire, G., Schwartz, E., Bennet, J. S., and Poncz, M., 1988, Structure of platelet glycoprotein IIIa. A common subunit for two different membrane receptors, J. Clin. Invest. 81: 1470–1475.

    PubMed  CAS  Google Scholar 

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  1. DRF/Laboratory of Hematology, Unité 217 INSERM, Center for Nuclear Studies, F38041, Grenoble Cedex, France

    Alain Duperray, Rolande Berthier & Gérard Marguerie

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  1. Alain Duperray
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  2. Rolande Berthier
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  1. North East Thames Regional Transfusion Centre, Brentwood, Essex, England

    J. R. Harris

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Duperray, A., Berthier, R., Marguerie, G. (1991). Biosynthesis and Processing of Platelet Glycoproteins in Megakaryocytes. In: Harris, J.R. (eds) Megakaryocytes, Platelets, Macrophages, and Eosinophils. Blood Cell Biochemistry, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9531-8_2

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