Expression of Human Erythrocyte Membrane Protein Antigens during Erythroid Differentiation

  • Marilyn J. Telen
Part of the Blood Cell Biochemistry book series (BLBI, volume 1)


Recent advances in hybridoma technology, fluorescence-activated cell sorting, and bone marrow culture techniques have led to investigations of the manner in which blood group antigens and other cell surface molecules are expressed during hematopoietic differentiation. These studies have demonstrated expression by erythroid progenitors and precursors of molecules or epitopes unique to the erythroid lineage as well as of other molecules whose expression is shared by precursor cells of other lineages. Some antigens are expressed by progenitor cells but lost during differentiation, while other antigens appear first during intermediate and late stages of erythropoiesis.


Paroxysmal Nocturnal Hemoglobinuria Erythroid Cell Erythroid Progenitor Erythroid Differentiation Blood Group Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allen, R. W., and Hoover, B. A., 1983, Reorientation of membrane polypeptides during erythrocyte maturation, Blood 61: 803–806.PubMedGoogle Scholar
  2. Andreasen, R. B., and Olsson, L., 1987, Identification and characterization of an antigen specific for normal erythroid precursor cells and its application in diagnosis of erythroleukemia, Leuk. Res. 11: 1045–1053.PubMedCrossRefGoogle Scholar
  3. Anstee, D. J. 1986, Red cell membrane antigens, in: Red Cell Antigens and Antibodies (G. Garratty, ed.), pp. 1–15, American Association of Blood Banks, Arlington, Va.Google Scholar
  4. Anstee, D. J., Parsons, S. F., Ridgwell, K., Tanner, M. J. A., Merry, A. H., Thomson, E. E., Judson, P. A., Johnson, P., Bates, S., and Fraser, I. D., 1984, Two individuals with elliptocytic red cells apparently lack three minor erythrocyte membrane sialoglycoproteins, Biochem. J. 218: 615–619.Google Scholar
  5. Bettaieb, A., Villeval, J. L., Kieffer, N., Farace, F., Edelman, L., Cartron, J. P., and Vainchenker, W., 1987, Early erythroid markers as probes for normal and leukaemic erythroid differentiation, Ann. Inst. Pa.steurlImmunol. 138: 877–883.CrossRefGoogle Scholar
  6. Bodger, M. P., Izaguirre, C. A., Blacklock, H. A., and Hoffbrand, A. V., 1983, Surface antigenic determinants on human pluripotent and unipotent hematopoietic progenitor cells, Blood 61: 1006–1010.PubMedGoogle Scholar
  7. Chow, F. L., Hall, S. E., Rosse, W. F., and Telen, M. J., 1986, Separation of the acetylcholinesterase-deficient red cells in paroxysmal nocturnal hemoglobinuria, Blood 67: 893–897.Google Scholar
  8. Civin, C. I., and Loken, M. R., 1987, Cell surface antigens on human marrow cells: Dissection of hematopoietic development using monoclonal antibodies and multiparameter flow cytometry, Int. J. Cell Cloning 5: 267–288.Google Scholar
  9. Colin, Y., Rahuel, C., London, J., Romeo, P. H., d’Auriol, L., Galibert, F., and Cartron, J. P., 1986, Isolation of cDNA clones and complete amino acid sequence of human erythrocyte glycophorin C, J. Biol. Chem. 261: 229–233.PubMedGoogle Scholar
  10. Colin, Y., Kim, C. L. V., Tsapis, A., Cierge, M., d’Auriol, L., London, J., Galibert, F., and Cartron, J. P., 1989, Human erythrocyte glycophorin C, J. Biol. Chem. 264: 3773–3780.PubMedGoogle Scholar
  11. Connor, J., and Schroit, A. J., 1988, Transbilayer movement of phosphatidylserine in erythrocytes: Inhibition of transport and preferential labeling of a 31,000-dalton protein by sulthydryl reactive reagents, Biochemistry 27: 848–851.PubMedCrossRefGoogle Scholar
  12. Darling, S. M., Banting, G. S., Pym, B., Wolfe, J., and Goodfellow, P. N., 1986, Cloning an expressed gene shared by the human sex chromosomes, Proc. Natl. Acad. Sci. USA 83:135–139.Google Scholar
  13. Dessypris, E. N., Clark, D. A., McKee, L. C., Jr., and Krantz, S. B., 1983, Increased sensitivity to complement of erythroid and myeloid progenitors in paroxysmal nocturnal hemoglobinuria, N. Engl. J. Med. 309: 690–693.PubMedCrossRefGoogle Scholar
  14. Edelman, P., Vinci, G., Villeval, J. L., Vainchenker, W., Henri, A., Miglierina, R., Rouger, P., Reviron, J., Breton-Gorius, J., Sureau, C., and Edelman, L., 1986, A monoclonal antibody against an erythrocyte ontogenic antigen identifies fetal and adult erythroid progenitors, Blood 67: 56–63.PubMedGoogle Scholar
  15. Ekblom, M., Gahmberg, C. G., and Andersson, L. C., 1985, Late expression of M and N antigens on glycophorin A during erythroid differentiation, Blood 66: 233–236.PubMedGoogle Scholar
  16. Falkenburg, J. H., Fibbe, W. E., van der Vaart-Duinkerken, N., Nichols, M. E., Rubinstein, P., and Jansen, J., 1985, Human erythroid progenitor cells express rhesus antigens, Blood 66: 660–663.PubMedGoogle Scholar
  17. Fauser, A. A., and Messner, H. A., 1978, Granuloerythropoietic colonies in human bone marrow, peripheral blood, and cord blood, Blood 52: 1243–1248.PubMedGoogle Scholar
  18. Frydecka, I. Brodzka, W., Lawinska, B., and Sciborski, R., 1984, Clinical course and results of treatment of erythroleukemia, Folia Haematol. (Leipzig) 111:282–289.Google Scholar
  19. Fukuda, M., and Fukuda, M. N., 1984, Cell surface glycoproteins and carbohydrate antigens in development and differentiation of human erythroid cells in The Biology of Glycoproteins (R. J. Ivatt, ed.), pp. 183–234, Plenum Press, New York.Google Scholar
  20. Fukuda, M., Fukuda, M. N., and Hakomori, S. I., 1979, Developmental change and genetic defect in the carbohydrate structure of band 3 glycoprotein of human erythrocyte membrane, J. Biol. Chem. 254: 37003703.Google Scholar
  21. Fukuda, M., Fukuda, M. N., Papayannopoulou, T., and Hakomori, S., 1980, Membrane differentiation in human erythroid cells: Unique profiles of cell surface glycoproteins expressed in erythroblasts in vitro from three ontogenic stages of differentiation and maturation, Proc. Natl. Acad. Sci. USA 77: 3474–3478.Google Scholar
  22. Gahmberg, C. G., 1983, Molecular characterization of the human red cell Rh0(D) antigen, Eur. Mol. Biol. Organ. J. 2: 223–227.Google Scholar
  23. Gahmberg, C. G., Jokinen, M., and Andersson, L. C., 1978, Expression of the major sialoglycoprotein (glycophorin) on erythroid cells in human bone marrow, Blood 52: 379–387.PubMedGoogle Scholar
  24. Goodfellow, P. J., Darling, S., Banting, G., Pym, B., Mondello, C., and Goodfellow, P. N., 1987, Pseudoautosomal genes in man, Development 101 (Suppl.): 119–125.PubMedGoogle Scholar
  25. Goodfellow, P. N., and Tippett, P., 1981, A human quantitative polymorphism related to Xg blood groups, Nature 289: 404–405.PubMedCrossRefGoogle Scholar
  26. Goodfellow, P. N., Banting, G., Sheer, D., Ropers, H. H., Caine, A., Ferguson-Smith, M. A., Povey, S., and Voss, R., 1983, Genetic evidence that a Y-linked gene in man is homologous to a gene on the X chromosome, Nature 302: 346–349.Google Scholar
  27. Goodfellow, P. N., Pym, B., Mohandas, T., and Shapiro, L. J., 1984, The cell surface antigen locus, MIC2X, escapes X-inactivation, Am. J. Hum. Genet. 36: 777–782.PubMedGoogle Scholar
  28. Gordon, M. Y., Barrett, A. J., and Gordon-Smith, E. C., 1985, Bone Marrow Disorders: The Biological Basis of Clinical Problems,pp. 20–59, Blackwell, Oxford.Google Scholar
  29. Greaves, M. F., Sieff, C., and Edwards, P. A. W., 1983, Monoclonal antiglycophorin as a probe for erythroleukemias, Blood 61: 645–651.PubMedGoogle Scholar
  30. Greaves, M. F., Katz, F. E., Myers, C. D., Davies, L., and Sieff, C., 1985, Selective expression of cell surface antigens on human haematopoietic progenitor cells, in: Hematopoietic Stem Cell Physiology ( E. P. Cronkite, N. Dainiak, R. P. McCaffrey, J. Palek, and P. J. Quesenberry, eds.), pp. 301–315, Liss, New York.Google Scholar
  31. Gupta, A. D., Samoszuk, M. K., Papayannopoulou, T., and Stamatoyannopoulos, G., 1985, SFL 23.6: A monoclonal antibody reactive with CFU-E, erythroblasts, and erythrocytes, Blood 66: 522–526.PubMedGoogle Scholar
  32. Harvey, J., Parsons, S. F., Anstee, D. J., and Bradley, B. A., 1988, Evidence for the occurrence of human erythrocyte membrane sialoglycoproteins in human kidney endothelial cells, Vox Sang. 55: 104–108.PubMedCrossRefGoogle Scholar
  33. Ievleva, E. S., Endelhardt, N. V., and Abelev, G. I., 1976, Specific antigen of murine erythroblasts, Int. J. Cancer 17: 798–805.PubMedCrossRefGoogle Scholar
  34. Issitt, P. D., 1988, Genetics of the Rh blood group system: Some current concepts, Med. Lab. Sci. 395:404. Issitt, P. D., 1989, The Rh blood group system, 1988: Eight new„antigens in nine years and some observations on the biochemistry and genetics of the system, Transf. Med. Rev. 3: 1–12.CrossRefGoogle Scholar
  35. Kanamaru, A., Okuda, K., Ueda, E., Kitani, T., Kinoshita, T., and Nagai, T., 1988, Different distribution of decay-accelerating factor on hematopoietic progenitors from normal individuals and patients with paroxysmal nocturnal hemoglobinuria, Blood 72: 507–511.PubMedGoogle Scholar
  36. Kay, M. M., Bosman, G. J., and Lawrence, C., 1988, Functional topography of band 3: Specific structural alteration linked to functional aberrations in human erythrocytes, Proc. Natl. Acad. Sci. USA 85: 492–496.PubMedCrossRefGoogle Scholar
  37. Latron, F., Blanchard, D., and Cartron, J. P., 1987, Immunochemical characterization of the human blood cell membrane glycoprotein recognized by monoclonal antibody 12E7, Biochem. J. 247: 757–764.PubMedGoogle Scholar
  38. Liszka, K., Majdic, O., Bettelheim, P., and Knapp, W., 1983, Glycophorin A expression in malignant hematopoiesis, Am. J. Hematol. 15: 219–226.PubMedCrossRefGoogle Scholar
  39. Loken, M. R., Civin, C. I., Bigbee, W. L., Langlois, R. G., and Jensen, R. H., 1987, Coordinate glycosylation and cell surface expression of glycophorin during normal human erythropoiesis, Blood 70: 1959–1961.Google Scholar
  40. Mattei, M. G., Colin, Y., Le Van Kim, C., Mattei, J. F., and Cartron, J. P., 1986, Localization of the gene for human erythrocyte glycophorin C to chromosome 2, q14—q21, Hum. Genet. 74: 420–422.PubMedCrossRefGoogle Scholar
  41. Mechetner, E. B., Tonevitsky, A. G., Ievleva, E. S., and Rozinova, E. N., 1987, Identification of a human erythroid cell surface antigen by monoclonal antibody HAE9, Exp. Hematol. 15: 355–359.PubMedGoogle Scholar
  42. Moore, J. G., Frank, M. M., Muller-Eberhard, H. J., and Young, N. S., 1985, Decay-accelerating factor is present on paroxysmal nocturnal hemoglobinuria erythroid progenitors and lost during erythropoiesis in vitro, J. Exp. Med. 162:1182–1192.Google Scholar
  43. Moore, S., and Green, C., 1987, The identification of specific rhesus polypeptide/blood-group-ABH active glycoprotein complexes in the human red cell membrane, Biochem. J. 244: 735–741.PubMedGoogle Scholar
  44. Nakahata, T., and Ogawa, M., 1982, Identification in culture of a class of hemopoietic colony-forming units with extensive capability to self-renew and generate multipotential hemopoietic colonies, Proc. Natl. Acad. Sci. USA 79: 3843–3847.PubMedCrossRefGoogle Scholar
  45. O’Hara, C. J., Shumkak, K. H., and Price, G. B., 1978, The i antigen on hyman myeloid progenitors, Clin. Immunol. Immunopathol. 10: 420–426.PubMedCrossRefGoogle Scholar
  46. Rahuel, C., London, J., d’Auriol, L., Mattei, M. G., Toumamille, C., Skrzynia, C., Levouc, Y., Galibert, F., and Cartron, J. P., 1988, Characterization of cDNA clones for human glycophorin A, Eur. J. Biochem 172:147–153.Google Scholar
  47. Rearden, A., and Chiu, P., 1983, Lack of rhesus antigen expression by human committed erythroid progenitors, Blood 61: 525–529.PubMedGoogle Scholar
  48. Rearden, A., and Masouredis, S. P., 1977, Blood group D antigen content of nucleated red cell precursors, Blood 50: 981–986.PubMedGoogle Scholar
  49. Reid, M. E., Anstee, D. J., Tanner, M. J. A., Ridgwell, K., and Nurse, G. T., 1987, Structural relationships between human erythrocyte sialoglycoproteins (3 and y and abnormal sialoglycoproteins found in certain rare human erythrocyte variants lacking Gerbich blood-group antigen(s), Biochem. J. 244: 123–128.PubMedGoogle Scholar
  50. Ridgwell, K., Roberts, S. J., Tanner, M. J. A., and Anstee, D. J., 1983, Absence of two membrane proteins containing extracellular thiol groups in Rh,,,,ii human erythrocytes, Biochem. J. 213: 267–269.PubMedGoogle Scholar
  51. Robinson, J., Sieff, C., Delia, D., Edwards, P. A. W., and Greaves, M., 1981, Expression of cell-surface HLADR, HLA-ABC and glycophorin during erythroid differentiation, Nature 289: 68–71.PubMedCrossRefGoogle Scholar
  52. Sakurai, T., Hara, H., and Nagai, K., 1988, Anti-K562 cell monoclonal antibody RTF8X recognizes tumor-associated antigen of erythroid lineage, Blood 72: 1487–1491.PubMedGoogle Scholar
  53. Siebert, P. D., and Fukuda, M., 1986, Human glycophorin A and B are encoded by separate, single copy genes coordinately regulated by a tumor-promoting phorbol ester, J. Biol. Chem. 261: 12433–12436.PubMedGoogle Scholar
  54. Sieff, C., 1986, Membrane antigen expression during hematopoietic differentiation, CRC Crit. Rev. Oncol. Hematol. 5: 1–36.CrossRefGoogle Scholar
  55. Sieff, C., and Greaves, M. F., 1983, Cell surface characteristics of hematopoietic cells, in: Red Cell Membrane Glycoconjugates and Related Genetic Markers (J. P. Cartron, P. Rouger, and C. Salmon, eds.), pp. 243ff, Librairie Arnette, Paris.Google Scholar
  56. Sieff, C., Bicknell, D., Caine, G., Robinson, J., Lam, G., and Greaves, M. F., 1982, Changes in cell surface antigen expression during hematopoietic differentiation, Blood 60: 703–713.PubMedGoogle Scholar
  57. Steck, T. L., 1978, The band 3 protein of the human red cell. A review, J. Supramol. 8: 311–423.Google Scholar
  58. Tate, C. G., and Tanner, M. J. A., 1988, Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins a and S, Biochem. J. 254: 743–750.PubMedGoogle Scholar
  59. Telen, M. J., and Borowitz, M. J., 1989, Glycophorin C as a marker of erythroid differentiation in non-lymphoid leukemias, manuscript in preparation.Google Scholar
  60. Telen, M. J., and Chasis, J. A., 1989, Relationship of the human erythrocyte Wrb antigen to an interaction between glycophorin A and band 3, submitted for publication.Google Scholar
  61. Telen, M. J., Scearce, R. M., and Haynes, B. F., 1987, Human erythrocyte antigens. III. Characterization of a panel of murine monoclonal antibodies that react with human erythrocyte and erythroid precursor membranes, Vox Sang. 52: 236–243.PubMedCrossRefGoogle Scholar
  62. Telen, M. J., Hall, S. E., Green, A. M., Moulds, J. J., and Rosse, W. F., 1988, Identification of human erythrocyte blood group antigens on decay-accelerating factor (DAF) and an erythrocyte phenotype negative for DAF, J. Exp. Med. 167: 1993–1998.PubMedCrossRefGoogle Scholar
  63. Vainchenker, W., Testa, U., Rochant, H., Titeux, M., Henri, A., Bouguet, J., and Breton-Gorius, J., 1981, Cellular regulation of i and I antigen expression in human erythroblasts grown in vitro, Stem Cells 1: 97110.Google Scholar
  64. Villeval, J. L., Testa, U., Vinci, G., Tonthat, H., Bettaieb, A., Titeux, M., Cramer, P., Edelman, L., Rochant, H., Breton-Gorius, J., and Vainchenker, W., 1985, Carbonic anhydrase I is an early specific marker of normal human erythroid differentiation, Blood 66: 1162–1170.PubMedGoogle Scholar
  65. Villeval, J. L., Cramer, P., Lemoine, F., Henri, A., Bettaieb, A., Bernaudin, F., Beuzard, Y., Berger, R., Flandrin, G., Breton-Gorius, J., and Vainchenker, W., 1986, Phenotype of early erythroblastic leukemias, Blood 68: 1167–1174.PubMedGoogle Scholar
  66. Walloch, J., Frankel, S., Hrisinko, M. A., and Weil, S. C., 1986, Carbonic anhydrase: A marker for the erythroid phenotype in acute nonlymphocytic leukemia, Blood 68: 304–306.PubMedGoogle Scholar
  67. Williams, B. P., Daniels, G. L., Pym, B., Sheer, D., Povey, S., Okubo, Y., Andrews, P. W., and Goodfellow, P. N., 1988, Biochemical and genetic analysis of the Oka blood group antigen, Immunogenetics 27: 32 2329.Google Scholar
  68. Yokochi, T., Brice, M., Rabinovitch, P. S., Papayannopoulou, T., and Stamatoyannopoulos, G., 1984, Monoclonal antibodies detecting antigenic determinants with restricted expression on erythroid cells: From the erythroid committed progenitor level to the mature erythroblast, Blood 63: 1376–1384.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Marilyn J. Telen
    • 1
  1. 1.Division of Hematology/Oncology, Department of Medicine, and Immunohematology Laboratory, Transfusion ServiceDuke University Medical CenterDurhamUSA

Personalised recommendations