Rat Mast Cells
The correlation of histamine content and mast cell counts in various tissues by Riley and West (1953) implicated mast cells in the biology of histamine long after the effect of histamine on vascular permeability and smooth muscle contraction were known. The histamine content, 5-hydroxytryptamine (5-HT) content, and mast cell numbers were shown to vary in parallel from various regions of the skin in rats (West, 1959). Histamine releasers given to rats produced edema in characteristic skin locations corresponding to those containing mast cells and known to be rich in histamine (Benditt et al., 1955a; West, 1959). Research on rat mast cells was facilitated by the ability to obtain large numbers of pure cells from the peritoneal cavity for these studies (Padawer and Gordan, 1955). Only recently have readily available sources of purified populations of normal mast cells and basophils from other species been developed for this purpose. Rat peritoneal mast cells provided a key source of mast cells that allowed a number of important investigations to proceed before other sources of normal mast cells and basophils were developed.
KeywordsMast Cell Peritoneal Mast Cell Granule Membrane Intramembranous Particle Mast Cell Granule
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